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•  Welcome to the last enrollees!

  Change Page: < 12 | Showing page 2 of 2, messages 21 to 40 of 40 - powered by ASPPlayground.NET Forum Trial Version Author Message THEJRC Total Posts : 1006 Scores: 4 Reward points : 555 Joined: 10/23/2009 Location: Colorado Springs, CO, US Status: offline MBI: 210 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Welcome to the last enrollees! Sunday, July 31, 2011 9:23 AM (permalink) 0 Its not that Io cannot be grown from a FAF dish, it's that Iso is *incredibly* difficult to grow from a FAF dish!!  The algae simply loses upper hand to pretty much everything when stressed and gets outcompeted rather quickly.  I've managed on a few occassions but it's a very slow ramp up process.  Nanno and Tet you can easily scrape out into a 16 ounce bottle or 1000ml erlinmyer and split up to larger containers within a pretty quick pace...  Iso I have to scrape into 50ml tubes and babysit over a long period.  Usually takes about a month to ramp Iso from disc to 1000ml cultures (maintaining multiples of course!)   Carolina biological supply ships liquid cultures of Iso for around $8 a culture and it's been my preferred source for Iso when I feel the need to fight with this painful species...  most times I'm usin reeds Iso1800 line Pelagically yours, ~J         #21 Arc Katana Total Posts : 929 Scores: 19 Reward points : 1293 Joined: 10/20/2009 Status: offline MBI Site: Marine Breeding Initiative (MBI) Re:Welcome to the last enrollees! Sunday, July 31, 2011 9:27 AM (permalink) 0 Would using paste and slightly bubbling it so it stays in suspension work for stuff that feeds on Iso?  I'd rather go all paste/dry is possible, less crap to worry about!   #22 THEJRC Total Posts : 1006 Scores: 4 Reward points : 555 Joined: 10/23/2009 Location: Colorado Springs, CO, US Status: offline MBI: 210 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Welcome to the last enrollees! Sunday, July 31, 2011 9:34 AM (permalink) 0 generally it does Brett, though I must stress to everyone that Iso is not the magic bullet...  Variety is the key!   Regardless of whether your going paste or live, try and use multiple species as it will help round out the nutrition and offer a variety of cell sizes / shapes for the target to choose from.   My favorite mixes typically contain live Rhodomonas, live Nanno, live Gymnodinium, and Iso 1800 paste.  Think of the Gymnodinium as an O. marina substitute, it's another dinoflagellate that is easily obtained and tends to do extremely well in culture (then again it's also the evil red tide lol).   The big key though is in the variety!!  One of the main reasons why products like FAF's roti rich and the new line of products from Reed do so well is that they are not unicultured. Pelagically yours, ~J         #23 Arc Katana Total Posts : 929 Scores: 19 Reward points : 1293 Joined: 10/20/2009 Status: offline MBI Site: Marine Breeding Initiative (MBI) Re:Welcome to the last enrollees! Sunday, July 31, 2011 9:45 AM (permalink) 0 I was thinking of Iso paste and then throwing in the live greenwater (no idea of species) that I can make from the fresh saltwater here.  I figure I'll be hitting enough nutrients that way for 'pods. (well hoping!   #24 chuenwe Total Posts : 97 Scores: 0 Reward points : 113 Joined: 7/9/2011 Location: Huntington Sta, NY, US Status: offline MBI: 75 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Welcome to the last enrollees! Sunday, July 31, 2011 11:38 AM (permalink) 0 Originally Posted by KathyL I've heard from others, and it has been my experience, that Iso cannot be grown from a FAF dish.  Nanno yes, Iso no.   The interesting thing is that I followed their instruction and used the entire dish to start a culture with just 500ml of salt water.  And then I saw the dish still had a tiny little bit of brown stuff of it, so I scraped it real clean and pour in the tiny bit of scraped stuff on to a second 2L bottle and completely filled with 2L of 1.018 water.   The 500ml one that followed their instruction stayed clean for over a week then turned green.  While the 2L bottle with the tiny bit of Iso turned chocolate gradually during the week.  By 2nd week, the chocolate color got quite dark so I splited it.  Then all hell broke loose.  Everything turned green in just a week.   Originally Posted by THEJRC   generally it does Brett, though I must stress to everyone that Iso is not the magic bullet...  Variety is the key!      I already have nano and tet going pretty well.  just want to add iso into the mix to give it enough variety. Originally Posted by Arc Katana     I'd rather go all paste/dry is possible, less crap to worry about!      I'll be on the complete opposite of the spectrum.  Since my wife really doesn't want me to order any more "stuff", I have to grow everything my own.  Once I have a culture going, hopefully salt is the only thing that I'll have to order.     #25 THEJRC Total Posts : 1006 Scores: 4 Reward points : 555 Joined: 10/23/2009 Location: Colorado Springs, CO, US Status: offline MBI: 210 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Welcome to the last enrollees! Sunday, July 31, 2011 9:33 PM (permalink) 0 Originally Posted by chuenwe The 500ml one that followed their instruction stayed clean for over a week then turned green.  While the 2L bottle with the tiny bit of Iso turned chocolate gradually during the week.  By 2nd week, the chocolate color got quite dark so I splited it.  Then all hell broke loose.  Everything turned green in just a week.   yup, as Gresham says anything with crisis in the name is bound to be interesting..  this is typical behavior as the cells stress easily.  I learned long ago to split based on timing regardless of culture color and density (recommendation by Hoff) and it has proven to be a good method for me.  You may try again but focus on splits at 8-10 days regardless of color or density.  Starting small and staying small until confident of the cultures density is always a good bet.  There is nothing wrong with splitting into smaller cultures providing you have space.  Algae are just as alive as any other organism, dont think of it as splitting your cultures... think along the lines of providing a more comfortable environment for 20 million pets. Originally Posted by I'll be on the complete opposite of the spectrum.  Since my wife really doesn't want me to order any more "stuff", I have to grow everything my own.  Once I have a culture going, hopefully salt is the only thing that I'll have to order. Cant wait to see this one!!  Most people, even the advanced hobbyists have thrown out culturing due to the time and efforts involved and have thus gone the routes of the prepared feeds.  I cannot knock the prepared feeds especially lately as the last two years has brought incredible innovation to the market.  Then again I culture live algae because I *love* algae!!  It will be interesting to see what your thoughts are when you begin to delve into different species, especially the dino's and the Oxyrhiis as they are prolific and voracious to boot.   Necessity may be the mother of invention but observation is the mother of creativity!  Just wait till you see what's upstream! Pelagically yours, ~J         #26 JimWelsh Total Posts : 1426 Scores: 14 Reward points : 1486 Joined: 1/22/2010 Location: Angwin, CA, US Status: offline MBI: 1845 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Welcome to the last enrollees! Sunday, July 31, 2011 9:54 PM (permalink) 0 Originally Posted by THEJRC Its not that Io cannot be grown from a FAF dish, it's that Iso is *incredibly* difficult to grow from a FAF dish!!  The algae simply loses upper hand to pretty much everything when stressed and gets outcompeted rather quickly.  I've managed on a few occassions but it's a very slow ramp up process.  Nanno and Tet you can easily scrape out into a 16 ounce bottle or 1000ml erlinmyer and split up to larger containers within a pretty quick pace...  Iso I have to scrape into 50ml tubes and babysit over a long period.  Usually takes about a month to ramp Iso from disc to 1000ml cultures (maintaining multiples of course!) Carolina biological supply ships liquid cultures of Iso for around $8 a culture and it's been my preferred source for Iso when I feel the need to fight with this painful species...  most times I'm usin reeds Iso1800 line Ditto that.  I've grown Nanno and Tetraselmis from FAF disks, but I've NEVER grown ISO from an FAF disk.  My preferred source is SeahorseSource.com (via Algagen) for ISO starters.   #27 Fishtal Total Posts : 5467 Scores: 37 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Welcome to the last enrollees! Tuesday, September 13, 2011 10:11 PM (permalink) 0 Originally Posted by Umm_fish? Tal: List is here. It doesn't look like it.   For some reason I'm unable to find the list of participants... http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #28 chuenwe Total Posts : 97 Scores: 0 Reward points : 113 Joined: 7/9/2011 Location: Huntington Sta, NY, US Status: offline MBI: 75 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Welcome to the last enrollees! Tuesday, September 13, 2011 10:47 PM (permalink) 0 Maybe from the forum's group member list?     #29 Fishtal Total Posts : 5467 Scores: 37 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Welcome to the last enrollees! Tuesday, September 13, 2011 10:52 PM (permalink) 0 Originally Posted by chuenwe Maybe from the forum's group member list? Thanks, I'm a bit slow sometimes. http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #30 GreshamH Total Posts : 757 Scores: 16 Reward points : 600 Joined: 4/27/2011 Location: SF Bay Area, CA Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Welcome to the last enrollees! Wednesday, September 14, 2011 12:09 AM (permalink) 0 Originally Posted by chuenwe   Once I have a culture going, hopefully salt is the only thing that I'll have to order.   Don't forget you'll need an F/2 to fuel the algal growth, I doubt you'll find that locally and Miracle Grow is no substitute despite what people say.       #31 JimWelsh Total Posts : 1426 Scores: 14 Reward points : 1486 Joined: 1/22/2010 Location: Angwin, CA, US Status: offline MBI: 1845 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Welcome to the last enrollees! Wednesday, September 14, 2011 12:34 AM (permalink) 0 Originally Posted by GreshamH Originally Posted by chuenwe Once I have a culture going, hopefully salt is the only thing that I'll have to order. Don't forget you'll need an F/2 to fuel the algal growth, I doubt you'll find that locally and Miracle Grow is no substitute despite what people say.   But this is Oxyrrhis marina, a hetertrophic dinoflagellate.  It is not, as far as I am aware, photosynthetic.  Neither Andy nor I have been using F/2 to grow this organism.  CCMP lists seawater + CCMP1320 (Dunaliella tertiolecta) as a food  Am I missing something here?   #32 GreshamH Total Posts : 757 Scores: 16 Reward points : 600 Joined: 4/27/2011 Location: SF Bay Area, CA Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Welcome to the last enrollees! Wednesday, September 14, 2011 1:40 AM (permalink) 0 Originally Posted by JimWelsh Originally Posted by GreshamH Originally Posted by chuenwe Once I have a culture going, hopefully salt is the only thing that I'll have to order. Don't forget you'll need an F/2 to fuel the algal growth, I doubt you'll find that locally and Miracle Grow is no substitute despite what people say. But this is Oxyrrhis marina, a hetertrophic dinoflagellate.  It is not, as far as I am aware, photosynthetic.  Neither Andy nor I have been using F/2 to grow this organism.  CCMP lists seawater + CCMP1320 (Dunaliella tertiolecta) as a food  Am I missing something here?   Yes you are missing something as there is source for oxy paste is there?  Read up above my post, you will see they were talking about growing ISO, pastes, etc     #33 JimWelsh Total Posts : 1426 Scores: 14 Reward points : 1486 Joined: 1/22/2010 Location: Angwin, CA, US Status: offline MBI: 1845 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Welcome to the last enrollees! Wednesday, September 14, 2011 2:14 AM (permalink) 0 OK, Gresham.  My bad.  Wesley was talking about live phyto as a feed, instead of paste.  I did miss something!  Thanks for the clarification.  You are right, he will need F/2, if he wants to use live phyto to feed things.   #34 THEJRC Total Posts : 1006 Scores: 4 Reward points : 555 Joined: 10/23/2009 Location: Colorado Springs, CO, US Status: offline MBI: 210 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Welcome to the last enrollees! Wednesday, September 14, 2011 7:10 AM (permalink) 0 Originally Posted by GreshamH Don't forget you'll need an F/2 to fuel the algal growth, I doubt you'll find that locally and Miracle Grow is no substitute despite what people say. +1  And yes I am bitter about the Miracle Grow.... lol   There are also other popular sources out there that recommend pre-mixing up batches of culture medium (saltwater and the F/2) so that you have it ready in a pinch.  This is also a bad idea as culture medium standing unused becomes a very nice target for contaminates, always best to mix up medium at the time of split keeps things cleaner! Pelagically yours, ~J         #35 chuenwe Total Posts : 97 Scores: 0 Reward points : 113 Joined: 7/9/2011 Location: Huntington Sta, NY, US Status: offline MBI: 75 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Welcome to the last enrollees! Wednesday, September 14, 2011 7:40 PM (permalink) 0 I just ordered 1kg of dry form of MICRO ALGAE GROW MASS PACK™ from FAF and it should be good for 10,000L in f/2 level.  Should last me awhile.       #36 THEJRC Total Posts : 1006 Scores: 4 Reward points : 555 Joined: 10/23/2009 Location: Colorado Springs, CO, US Status: offline MBI: 210 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Welcome to the last enrollees! Wednesday, September 14, 2011 9:28 PM (permalink) 0 Originally Posted by chuenwe I just ordered 1kg of dry form of MICRO ALGAE GROW MASS PACK™ from FAF and it should be good for 10,000L in f/2 level.  Should last me awhile.   LOL... you'd think.....   and then you become a card carrying member of AA... Algae Anonymous...  the cultures start to change... different species... different levels... more risks... more splits...   Next thing you know your on the phone begging and pleading for another mass pack....  maybe two.......   *sigh* Pelagically yours, ~J         #37 chuenwe Total Posts : 97 Scores: 0 Reward points : 113 Joined: 7/9/2011 Location: Huntington Sta, NY, US Status: offline MBI: 75 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Welcome to the last enrollees! Wednesday, September 14, 2011 9:36 PM (permalink) 0 LOL!!  Sounds like from an AA member himself.       #38 CableGuy Total Posts : 591 Scores: 7 Reward points : 731 Joined: 8/26/2010 Location: Saint Clair Shores, MI, US Status: offline MBI: 635 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Welcome to the last enrollees! Wednesday, September 21, 2011 5:16 PM (permalink) 0 I was able to squeeze in here as someone was too busy so thanks to those who helped me get in and I'll be doing a lot of reading and catching up so Im sorry if I am quiet. If I have questions I'll ask, thats for sure. (Sorry Tal   .... as you are the closest to me you might get the blunt end of those questions... as usual.) -Adam   #39 Fishtal Total Posts : 5467 Scores: 37 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Welcome to the last enrollees! Wednesday, September 21, 2011 5:43 PM (permalink) 0 Originally Posted by CableGuy I was able to squeeze in here as someone was too busy so thanks to those who helped me get in and I'll be doing a lot of reading and catching up so Im sorry if I am quiet. If I have questions I'll ask, thats for sure. (Sorry Tal   .... as you are the closest to me you might get the blunt end of those questions... as usual.) LOL. Welcome to 'pod land. http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #40 Online Bookmarks Sharing: Facebook Digg Google del.icio.us StumbleUpon Change Page: < 12 | Showing page 2 of 2, messages 21 to 40 of 40 - powered by ASPPlayground.NET Forum Trial Version

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