Culture Journal, Species: Isochrysis galbana

Re:Culture Journal, Species: Isochrysis galbana (shannpeach)

Tue, 23 Jul 2013 08:40:03 GMT

90+ days later and I still have the Iso going. In the summer it seems to be a bit more difficult to maintain, but it's been hanging in there:

I am working on moving all three bottles to the 3L instead of using 2L, but I hate to just buy the water bottles and then waste the water so I need to drink a lot of water before I will have enough bottles.

The only thing I have changed is that usually now I just use freshly mixed ASW instead of re-using broodstock water. It's more because I have been too lazy to sieve the broodstock water to get rid of the junk and I hate having buckets all over in various stages of phyto water prep. I still sterilize the freshly mixed ASW using the same method I always have (bleach and sodium thiosulfate).

Re:Culture Journal, Species: Isochrysis galbana (shannpeach)

Thu, 18 Apr 2013 12:21:12 GMT

The gold bottle in the center of the picture and the one on the far left are the two T-Iso bottles I currently have.

I just reworked my culture area a bit and now I can expand the number of T-Iso bottles I have to at least three, possibly four. This culture was actually started several weeks ago, but I am just getting around to making this culture journal now. I have some microscope pics from last night, I just need to make sure I put them on my phone and will post them shortly if I have.

Culture Journal, Species: Isochrysis galbana (shannpeach)

Thu, 18 Apr 2013 12:17:22 GMT

Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system

General
Species: Isochrysis galbana
Species description: golden-brown, motile, 1uM wide x 4-8uM long
Culture source (link if possible): Fellow hobbyist
If algae, CCMP # (Optional):
http://ccmp.bigelow.edu/
Culture Establishment Date: 4/18/2013
Continuation Date: 7/24/2013

Culturing Vessel Details
Salinity: 1.020
Temperature: Ambient room temp, currently ~72F
pH: Not measured

Vessel description: 2L soda bottles
Lighting description: Ambient light from southern facing window supplemented with 2-bulb fluorescent strip light
Lighting cycle: Sunrise to sunset, strip light is on from 6PM to 9PM
Aeration description: rolling bubble from rigid airline tubing

Methodologies
Split methodology: Once culture is dark enough (I don't wait until it is "chocolate brown," I split when it is more of a dark "gold" color) I harvest 2/3 of the bottle and replace with sterilized, fertilized broodstock tank water. Then changed to using sterilized freshly mixed ASW since it is easier to prepare.

Culture medium description:
1/2mL of Florida Aqua Farm's Microalgae grow per 1L of sterilized water

Cell count:
(if known)

Reference links:

Additional Information
(No Pictures or Videos in the Section Please)
Notes:
Water is sterilized using bleach and neutralized using sodium thiosulfate

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