Culture Journal, Species: Heterocapsa triquetra

Re:Culture Journal, Species: Heterocapsa triqueta (shannpeach)

Sat, 17 May 2014 12:07:28 GMT

It was a gift from a university lab. I can pass some along to you if you want

Re:Culture Journal, Species: Heterocapsa triqueta (KathyL)

Sat, 17 May 2014 11:35:17 GMT

So cool! I would love to try some. Where did you get it?

Re:Culture Journal, Species: Heterocapsa triqueta (dave w)

Sat, 17 May 2014 09:55:41 GMT

Keep up the good work. I've always wondered why more people didn't culture dinos.

Re:Culture Journal, Species: Heterocapsa triqueta (shannpeach)

Fri, 16 May 2014 22:43:09 GMT

I am finally getting this to grow is good numbers.

I may finally have enough to use to test as a possible food for Priolepis larvae.

Re:Culture Journal, Species: Heterocapsa triqueta (shannpeach)

Wed, 30 Apr 2014 11:48:43 GMT

This is very nerdy, but I really like looking at these guys under the microscope

I have been toying with different culture conditions and right now I have three cultures going. One of them, I am trying to see if feeding RG complete helps increase numbers, but if I can get it to grow in higher densities by just increasing the amount of light, then I would rather skip the extra feeding.

Re:Culture Journal, Species: Heterocapsa triqueta (shannpeach)

Thu, 17 Apr 2014 20:26:17 GMT

Took some more pics today that allowed me to get some measurements in these guys. I like the size and hope to try them as a food for rusty goby larvae once the cultures are going strong.

Re:Culture Journal, Species: Heterocapsa triqueta (shannpeach)

Tue, 15 Apr 2014 19:13:24 GMT

I split this and now have one culture that is not aerated and one that is.

Re:Culture Journal, Species: Heterocapsa triqueta (shannpeach)

Thu, 10 Apr 2014 08:58:24 GMT

Some pics of this nice, large dino
4x objective:

10x objective:

40x objective:

Culture Journal, Species: Heterocapsa triquetra (shannpeach)

Wed, 09 Apr 2014 08:37:45 GMT

Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system

General
Species: Heterocapsa triquetra
Species description: Mixotrophic dinoflagellate, golden in color
Culture source (link if possible):
If algae, CCMP # (Optional):
http://ccmp.bigelow.edu/
Culture Establishment Date: 4-8-2014
Continuation Date:

Culturing Vessel Details
Salinity: ~1.021
Temperature: ~64F
pH: Not measured

Vessel description: 500 mL bottle
Lighting description: Fluorescent strip light
Lighting cycle: 14 on, 10 off
Aeration description: None at the moment

Methodologies
Split methodology:

Culture medium description:
1 mL of FAF microalgae grow per 2L of ASW (sterilized with 0.5 mL of bleach per L for at least an hour, neutralized with 1.5 mL of 1M sodium thiosulfate per L)

Cell count:
(if known)

Reference links:
http://www.eos.ubc.ca/res...capsa/h_triquetra.html
Additional Information
(No Pictures or Videos in the Section Please)
Notes:
Taken from the Phytoplankton Encyclopedia:
-Mixotrophic, can reproduce sexually and asexually
-Cells are 16-30 μm long and 9-18 μm wide. The epitheca is conical with straight sides and a rounded end. The hypotheca has an asymmetrical horn and a unique protrusion. Cells do not have spines.
-non-toxic and is considered to be a high quality food for heterotrophic dinoflagellates, ciliates, and copepods

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