Re:Culture Journal, Species: Tetraselmis chuii (waldend)

Thu, 12 Feb 2015 09:01:37 GMT

Thanks Kathy. This one does seem pretty bullet proof. I love getting it so dark you cannot see into it.

Now, if only Iso were so easy!

Re:Culture Journal, Species: Tetraselmis chuii (KathyL)

Thu, 12 Feb 2015 08:59:14 GMT

Your culture looks great! I love tetraselmis! Who knew a plant could swim so well?

Re:Culture Journal, Species: Tetraselmis chuii (waldend)

Tue, 10 Feb 2015 22:08:41 GMT

Updated picture of the color.

Re:Culture Journal, Species: Tetraselmis chuii (waldend)

Tue, 10 Feb 2015 20:28:35 GMT

Video of the culture for continuation.

Re:Culture Journal, Species: Tetraselmis chuii (waldend)

Wed, 30 Jul 2014 10:36:36 GMT

Good color comparison to Iso. (A contaminated Iso is in background. I assume it's a Tet contamination since that is the only green I have).

Re:Culture Journal, Species: Tetraselmis chuii (waldend)

Wed, 30 Jul 2014 10:34:14 GMT

Start on 7/24. 3rd from right (light green)

Same pic from 7/28. Darkened nicely.

Culture Journal, Species: Tetraselmis chuii (waldend)

Wed, 30 Jul 2014 10:24:19 GMT

Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system

General
Species: Tetraselmis chuii
Species description: Green microalgae
Culture source (link if possible): MBI Conference (T. Weiss)
If algae, CCMP # (Optional):
[link=http://ccmp.bigelow.edu/]http://ccmp.bigelow.edu/[/link]
Culture Establishment Date:7/24/2014
Continuation Date: 12/31/2014

Culturing Vessel Details
Salinity: 1.024 currently, 1.020 initially.
Temperature: Ambient Room Temp (70°F-78°F in summer, 60°F-70°F in winter)
pH:Not monitored

Vessel description: Current is 2L clear plastic container with screw on lid. Hole drilled in lid to snugly fit a rigid airline. Initial was 1 Quart Wide-mouth canning jars. Plastic wrap cover held with rubberband around perimeter. Rigid airline poked through wrap to feed air and a small puncture for air release.
Lighting description: Single T8 bulb under-cabinet lighting fixture.
Lighting cycle: 18 on, 6 off. Off is in the afternoon to reduce temp during the warmest part of day (summer and winter) to keep a more consistent temperature.
Aeration description: Light boil to keep algae fully in suspension

Methodologies
Split methodology: Plan is to remove 75% every 7-10 days and add the remaining 25% to a new jar as an inoculation. Actual ended up being minimal splits every 3-4 weeks. Would add a second 20 drops of Micro Algae Grow if growth stalled. Color would be extremely dark green at time of split.

Culture medium description: Current is 2l of ASW sterilized with chlorine at .8ml/2l , allowed to sit for 24hrs and then neutralized with equal parts sodium thiosulfate. 20 drops Micro Algae Grow added per 2l. Initial was 1 litre of artificial saltwater newly mixed and aged ~ 24hrs. Sterilized in microwave until the water boils in the jar. Allowed to cool at room temp. 10 drops (~1/2ml) Micro Algae Grow added to the culture.

Cell count: Not known
(if known)

Reference links:

Additional Information
(No Pictures or Videos in the Section Please)
Notes:

You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.