Culture Journal, Species: Euterpina acutifrons

Re:Culture Journal, Species: Euterpina acutifrons (road8514)

Sun, 27 Sep 2015 22:40:30 GMT

These crashed. Will need to get another filter going.

Re:Culture Journal, Species: Euterpina acutifrons (clayton447)

Thu, 13 Aug 2015 11:21:53 GMT

Originally Posted by road8514

I am not sure. But I will keep them in salt so I don't have issues using them for salt water fish. I don't think they would handle quick change in salinity from culture vessels to fry rearing vessel.

You could always perform some simple salinity stress tests and see how they respond. Some copepods are more durable than others. I work with Tigriopus and the can easily tolerate going from 30ppt to 0ppt instantly, and stay in 0ppt for 30 minutes without mortalities. It takes about 15 minutes for them to snap out of the stress after they are put back into 30ppt; it's remarkable either way. I am also working with Apocyclops and I will be testing them with salinity shock treatments. That actually sounds barbaric! LOL

Chad

Re:Culture Journal, Species: Euterpina acutifrons (road8514)

Tue, 11 Aug 2015 21:41:31 GMT

I am not sure. But I will keep them in salt so I don't have issues using them for salt water fish. I don't think they would handle quick change in salinity from culture vessels to fry rearing vessel.

Re:Culture Journal, Species: Euterpina acutifrons (dave w)

Tue, 11 Aug 2015 20:30:40 GMT

If I remember correctly, this species can acclimate in salinities almost all the way down to fresh water. That might save you a little on the salt bill if you decide to use a low salinity algae as well.

Re:Culture Journal, Species: Euterpina acutifrons (road8514)

Tue, 11 Aug 2015 15:16:34 GMT

Culture Journal, Species: Euterpina acutifrons (road8514)

Tue, 11 Aug 2015 15:11:18 GMT

Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system

General
Species: Euterpina acutifrons
Species description: Harpacticoid
Culture source (link if possible): Waldend at 2015 MBI Workshop
If algae, CCMP # (Optional):
http://ccmp.bigelow.edu/
Culture Establishment Date: July 25, 2015
Continuation Date:

Culturing Vessel Details
Salinity: 1.021
Temperature: Room Temp
pH: unknown

Vessel description: 1.75 liter used juice container.
Lighting description: light from the phyto cultures, single spiral CFL bulb.
Lighting cycle: 14 on 10 off direct lighting
Aeration description:  Light air 1 bubble per second, just enough to keep water moving.

Methodologies
Split methodology: Culture is poured through a 23micron sieve to remove all copepods. They are then backwashed into a container with fresh ASW.

Culture medium description:
Culture is feed Tetraselmis as needed to tint the water.

Cell count: unknown
(if known)

Reference links:

Additional Information
(No Pictures or Videos in the Section Please)
Notes:
Water is sterilized using bleach and and dechlorinated using sodium thiosulfate.
Stock solution of sodium thiosulfate made following this info.
http://www.mbisite.org/fo...print=true

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