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Re:Breeding Journal, Species: Liopropoma mowbrayi
Sunday, July 31, 2011 8:38 PM
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Luis I had mentioned this to one or two folks at the conference, which seemed to go over fairly well. The dip is done for bacteria once eggs are collected. I plan on running a dosing pump into the larvae tank that will keep a steady amount of hydrogen peroxide into the tank to help with bacteria. What levels I am not sure yet - it will be trial and error! Maybe Todd G. can chime in with some of his secrets to getting them past the 48/72 hour stage!
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Re:Breeding Journal, Species: Liopropoma mowbrayi
Tuesday, August 2, 2011 6:37 PM
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Female is HUGE tonight. I'm hoping for a good spawn. The best I've had is just over 300 (304 if I remember correctly). Funny thing on the spawns - by re-arranging the tank they skip a night. I wonder if this could be used to get them to spawn every other night with bigger spawns? Something I'll have to play around with. Biggest thing is now I'm ready to for a large spawn. I'll be using hydrogen peroxide on the eggs in a bath for about 30 minutes, washing with new asw, then placing them into a 9 gallon tank with a heater at 81-82 degrees F. Hoping this gets me past the 72 hour mark. Tank will be bare except for heater and an extremely slow bubble (one per minute) to keep some water movement and prevent the heater from burning anything that gets to near. Pics later.
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Re:Breeding Journal, Species: Liopropoma mowbrayi
Tuesday, August 2, 2011 6:51 PM
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good luck!
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Re:Breeding Journal, Species: Liopropoma mowbrayi
Tuesday, August 2, 2011 8:37 PM
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Got about 150 or so eggs tonight, put them in H202 for about 30 minutes then into the hatching tank. I'll cover the tank sides tomorrow, and add a little more h202 to the tank to help with bacteria/fungus. Hopefully some pics of prolarvae in 48 hours!
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Re:Breeding Journal, Species: Liopropoma mowbrayi
Tuesday, August 2, 2011 9:37 PM
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Good luck!
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Re:Breeding Journal, Species: Liopropoma mowbrayi
Thursday, August 4, 2011 9:32 PM
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After doing some research, I think I've found the main issue to egg fecundity - broodstock diet. While they love eating pellets it just isn't good enough for consistent quality of spawns. While the can survive on what they are eating its not optimal. The new plan is the following: Culture mysid shrimp that are fed a very high HUFA diet and then feed the fish. In effect I'll be going for "gut loading" of the mysid. I've always had this in the back of my head that the diet is one of the main problems so it is time to address it. I'll also try to culture some of the grass/glass shrimp that are in the sea grass beds as well. If I can find some very large copepods, I'll try those also. Note I'll be switching to all live feeds as soon as possible for all broodstock, with dry / frozen as a fall back if I run out.
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Re:Breeding Journal, Species: Liopropoma mowbrayi
Friday, August 5, 2011 7:06 AM
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I've also seen differences in egg production in the wrasses based on number of times the broodstock was fed per day. They like lots of meals. Good luck!
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Breeding Journal, Species: Liopropoma mowbrayi
Friday, August 5, 2011 11:25 AM
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Simpler answer - PE MYSIS.
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Re:Breeding Journal, Species: Liopropoma mowbrayi
Friday, August 5, 2011 11:41 AM
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I've feed mysis - they'll eat it, but not real excited about it. They get bright pink when they hunt the live mysid/grass shrimp. Maybe the stuff I have is bad -I'll get some new stuff for them. Cheers
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Re:Breeding Journal, Species: Liopropoma mowbrayi
Sunday, August 7, 2011 7:25 AM
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So the first couple of batches of eggs I tried with peroxide had very low fertility - an example was the other night - 50ish eggs, with three being viable. Then last night I collected about 150, with nearly 100 viable. Seems to me that only the clear eggs are fertile, and ones with white dots aren't. That in itself is interesting, as if things are eating your infertile eggs, they hopefully aren't eating your good ones! Also the being clear might make it harder for predators to see your good eggs and thus helping your success. So I've gone down to just a couple of drops of peroxide in the egg holder. My line of thinking is to check the eggs every 24 hours or so, pull the white eggs (and hopefully slowing/stopping fungus for the rest). I'm also going to set up a new system for eggs (which I'll post later) and prolarvae and grow out. Crossing my fingers, I think I've got a decent shot on these guys now.
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Re:Breeding Journal, Species: Liopropoma mowbrayi
Sunday, August 7, 2011 9:10 PM
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Another huge batch tonight - must be nearly 300 eggs, about 150-175 were "clear", which bodes well for fertilization. The whiter they are, the less likely they are to be fertile. I've stopped collecting those now to see if thats another weapon to combat fungus. Should have some prolarvae in about 20-24 hours! (From last night)
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Re:Breeding Journal, Species: Liopropoma mowbrayi
Monday, August 8, 2011 1:06 PM
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So iodine didn't do it, hydrogen with iodine didn't do it.... breaking out the 3W UV's. They'll get here tomorrow from amazon. The fungus/bacteria thing is just getting old at this point! Trying some new things for the eggs tonight. Blargh!
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Re:Breeding Journal, Species: Liopropoma mowbrayi
Monday, August 8, 2011 2:03 PM
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Are you bleaching the container that you're incubating in between batches?
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Re:Breeding Journal, Species: Liopropoma mowbrayi
Monday, August 8, 2011 2:49 PM
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I've been using different containers every time Matt. Eggs still get the brown globs on them after 12 hours no matter what. I'm almost thinking that they need to be in kreisel so there is constant soft flow around them. Working on the new hatching system today. Its incredibly frustrating to see at least 3000 eggs now just get attacked by bacteria/fungus and nothing seems to stop it.
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Re:Breeding Journal, Species: Liopropoma mowbrayi
Monday, August 8, 2011 7:58 PM
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Biggest spawn ever of these guys. There was north of 300-350 eggs, and a good 60-70% were fertile! I've got the eggs sitting in MarOxy at an amount for 5gallons - the eggs are in 8 ounces of water! They will sit in here for about 15 minutes then I'll pick them out to wash em off in new ASW, then they go into new ASW with about 5ml of H202. If this doesn't work then... well I dunno
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Re:Breeding Journal, Species: Liopropoma mowbrayi
Tuesday, August 9, 2011 9:43 AM
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So 12 hours later - no fungus or bacteria on the eggs, even the infertile ones! Phew. Now I might have dosed too much MarOxy and thus killed the eggs, but at least I've got a reference to where to go from now on. The most encouraging aspect is that even the infertile eggs showed no signs of bacteria/fungus. Since they take about 50ish hours to hatch to prolarvae, I've still got another day an half or so to go before I know if they will. I have sampled some eggs and have seen growth in them, so here's hoping they will continue. Phew. I was at wits end with the damn fungus/bacteria. One hurdle down, 9,999 more to go (Edited for Spelling)
<message edited by Arc Katana on Tuesday, August 9, 2011 10:55 AM>
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Re:Breeding Journal, Species: Liopropoma mowbrayi
Tuesday, August 9, 2011 10:12 AM
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That's really promising so far. Went looking for active ingredients / etc on Maroxy just to see what it came up with. Got this: "Mardel MarOxyslowly releases small amounts of a stabilized oxygen-chlorine combination which, when used as directed, has an unusual ability to treat a wide variety of tropical fish diseases, especially fungal infections and certain common bacterial infections. MarOxy does not color the water and will not interfere with the biological filter." Sadly, I can't find the active ingredients anywhere. Are there any listed in the bottle?
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Re:Breeding Journal, Species: Liopropoma mowbrayi
Tuesday, August 9, 2011 10:48 AM
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RLTW 180 Gallon Mixed Reef Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8
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Re:Breeding Journal, Species: Liopropoma mowbrayi
Tuesday, August 9, 2011 10:54 AM
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Yeah the only thing I've ever found was "Chlorine oxide" - which is the same stuff they use for swimming pools, just a much lower dosage. I could just get some big pool tablets but no idea as to how much that is actually when broken up. 3W UV sterilizer came in today from Amazon - put one on the tank with with eggs, just stuck sponges over the outlet so it diffuses the water. You can't even see a ripple from the outlet. Pics of eggs before and after in a bit, got to transfer from microscope. Thanks all!
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Re:Breeding Journal, Species: Liopropoma mowbrayi
Tuesday, August 9, 2011 10:58 AM
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I also came across a lot of links to mouth wash lol. I guess that may work too.
RLTW 180 Gallon Mixed Reef Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8
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