Anderson,the dishes hold 150 ml,which gives an initial density of more than 1PL/ml.
Mindy,the amount of water is irrelevant because it is being exchanged at the rate of 1 drop/sec.,or a complete turnover every 50 min.This kind of container works well for experimental work,as they can be checked with a microscope.
And they are in a rot sieve which sits in the dish!53 mic mesh can hold most naups,something smaller will clog fast.
The purpose of this project is to solve the problem of prolarval mortality.Raising angels is far,very far.
Today in their 4th day,there were less deaths,about 20%.PL are well developed,with refringent eyes,many resting on the bottom,but others swim in midwater.Development seems faster,may be the temp is a bit higher.
Vibrio showed but at very low numbers:3 CFU.