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Re:Culture Journal, Species: Brachionus plicatilis
Wednesday, October 5, 2011 6:28 PM
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I am not missing the praise, every letter in every praise post has been passed onto the rest of the company However as we're in the middle of branding this product line, it's imperative that first adapters get the name right when talking about it. The internet is pretty permanent and Google doesn't know if you spelled it wrong, nor will others when looking it up.
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Re:Culture Journal, Species: Brachionus plicatilis
Wednesday, October 5, 2011 8:53 PM
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Just for you, I've changed all my misspellings. I understand the need. Sorry I messed up initially. Spelling has not been my thing lately, nor typing neither.
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Re:Culture Journal, Species: Brachionus plicatilis
Tuesday, October 18, 2011 6:42 PM
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Rotifers continue to do well indoors. RGcomplete is good stuff, but I'm almost out of it, and I want to buy some, as well as some TDO A, B1, and lots of C1, but I don't want to place an order until I can get some RGcomplete! I guess I'll call tomorrow to see if I can get some more. Rotifers also continue to do well, if not better in the mixed critter bucket. I am less attentive to water changes and feeding, yet it seems that there are more rots in the the mixed population than in the single population bucket, that I am actively monitoring.
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Re:Culture Journal, Species: Brachionus plicatilis
Tuesday, October 18, 2011 7:02 PM
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what all is in the mixed bucket?
RLTW 180 Gallon Mixed Reef Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8
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Re:Culture Journal, Species: Brachionus plicatilis
Saturday, October 29, 2011 2:56 PM
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A little of everything. Rotifers, M. salina, T. californicus, A. panamensis, ciliates, and hopefully some O marina. I've continued to feed this bucket, taking out a daily gallon full to feed aiptasia right now. It seems to thrive.
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Re:Culture Journal, Species: Brachionus plicatilis
Friday, November 4, 2011 10:24 PM
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i did a rough count of the rotifers only bucket, and got about 50 rots per ml. Not great, but not bad either.
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Re:Culture Journal, Species: Brachionus plicatilis
Saturday, November 12, 2011 5:40 PM
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And for my continuing culture, here are a picture and a video: click to see video: I seem to be struggling with focus today, so I hope you'll forgive an old woman.
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Re:Culture Journal, Species: Brachionus plicatilis
Monday, November 14, 2011 7:29 AM
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Rotifers seem to be going strong, and contaminating everything!
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Re:Culture Journal, Species: Brachionus plicatilis
Monday, November 14, 2011 7:16 PM
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awesome. So are they doing better since it's all mixed together?
RLTW 180 Gallon Mixed Reef Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8
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Re:Culture Journal, Species: Brachionus plicatilis
Tuesday, November 15, 2011 6:38 AM
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hard to say. I haven't been able to get a good count of either bucket. The mixed culture always has a lot of detritus in it as well as the lots of critters. The rot bucket has a contaminating tigger pod population. Not sure that it improves the rotifers. I am sometimes inconsistent in my husbandry.
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Re:Culture Journal, Species: Brachionus plicatilis
Monday, November 21, 2011 8:11 AM
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Last week I put the "rotifers only" culture through my "coffee" filter which is a plastic mesh, probably 300 microns or so. Also, I've changed my daily routine. At least once a day, instead of dipping out a gallon and replacing that water, I prepare a clean bucket with aproximately 1 gallon of clean culture water, then decant the culture bucket into it, thus removing the detritus that sinks to the bottom on a daily basis. Some detritus pours into the new bucket, but the the majority is removed. From the old culture bucket, I then enrich the rotifers, filter out the gunk, and feed the rotifers to my baby fish and aiptasia. Since I've started doing this, I've noticed a large number of 3 egg females and 2 egg females, however my density remains at about 50 rots /ml. I would like to double that. Yesterday and today, even though I've checked and have lots of 2 and 3 egg females, the water is not clearing as fast as before. Still at 50 rots per ml. Not sure what is happening.
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Re:Culture Journal, Species: Brachionus plicatilis
Monday, November 21, 2011 10:44 AM
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Originally Posted by KathyL
At least once a day, instead of dipping out a gallon and replacing that water, I prepare a clean bucket with aproximately 1 gallon of clean culture water, then decant the culture bucket into it, thus removing the detritus that sinks to the bottom on a daily basis. Some detritus pours into the new bucket, but the the majority is removed. Hmm, that sure sounds easier than bottom siphoning the 5 gallon tanks like I've been doing. One point for bucket vs aquarium. I think they are tied now.
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Re:Culture Journal, Species: Brachionus plicatilis
Monday, November 21, 2011 12:58 PM
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If you still want to do tanks, try running a fine brine shrimp net across the bottom. I replaced one net with polyester organza from the fabric store, and I never siphoned again. The net collects the gunk in one or two swoops, and I can rinse it out in the sink.
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Re:Culture Journal, Species: Brachionus plicatilis
Monday, February 20, 2012 12:56 PM
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Well, despite daily bucket exchanges, and frequent feedings, I still ran out of rotifers for my clownfish, and I'm expanding right now, so there are a lot of babies to feed. I tried the T device that Gresham is working on, comparing it to just an open bucket with an airline, and there was no great improvement. I think it is still being tweaked. I think that air is more difficult to deliver to this device, although it improves if the bucket is nearly full. I'm trying something else right now with some success. I'm using a 17 gallon storage container, right now filled to only 8 gallons. I acquired a supplement of rots from my friend at the university, and have been able to gradually feed more, and get a better concentration of rotifers. I haven't counted yet, but I can tell when I harvest them that there are more on the filter. 3/4 gallon of the new culture is giving me more than 4 gallons did of the old culture, and there is no gunk in it. My friend at the U gave me a rather large sheet of the polyfilter that Reed recommends as an aggregated algae attractant. I've attached it to a needlepoint sheet, and this lives in the container, submerged, until I rinse it under the full force of the tap at the sink. I let it drain, and scrub the sides and bottom of the container with a toilet brush reserved for this purpose. Back it goes in after the brushing. The container has stayed relatively clean, compared to the bucket method. The filter material does take out some rotifers, but one must do that daily anyway. The daily spraying doesn't completely clean it, so I wonder how long it will be before it will need to be replaced. I am using more air than before, with an open ended rigid tube. The T device had an airstone, but the larger container sees a lot of water movement with the central airline and a lot of flow. I am probably using 10-12 ml of RGcomplete daily, and since i am home, I feed every few hours if I notice that it is clearing a bit. I should count them.
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Re:Culture Journal, Species: Brachionus plicatilis
Monday, February 20, 2012 1:25 PM
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WELL THAT WAS INTERESTING! I counted a ml of the culture and got 35 rots per ml. Hmm, seems low. Counted again, same thing. Counted a third time, but took the sample from the floor of the container. There were too many rotifers to count, perhaps 1000 per ml. !!!!! Took a sample from the filter, and there were, again, too many to count. These were dirtier, whereas the floor sample was clean. This makes me think that the rotifers appreciate a little substrate to hang around in. Perhaps we should try regular filter floss in addition to the filtering rotifer floss.
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Re:Culture Journal, Species: Brachionus plicatilis
Monday, February 20, 2012 1:28 PM
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Also, in my old method of decanting the old bucket into a clean bucket each day, I was perhaps removing more rotifers than I thought, and that may be why I would see the numbers dwindle. If they are more concentrated at the bottom of the containers we are growing them in, we should brush and stir BEFORE we take out the 25-33% of the culture each day.
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Re:Culture Journal, Species: Brachionus plicatilis
Tuesday, February 28, 2012 6:43 AM
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This new method with the larger container and the rotifer floss and the frequent feeding is working well. I have lots of rotifers, and the container stays pretty clean. I just broke down and ordered a peristaltic pump from Bulk Reef Supply, because I am now spending more time with the rotifers than I am with the fish, and this constant monitering and feeding will have to stop. Someday, I hope to be employed again….
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Re:Culture Journal, Species: Brachionus plicatilis
Sunday, March 4, 2012 11:46 PM
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After several days of fiddling with the pump and the timer to regulate the pump, I am reasonably well trained to keep this thing going, and hopefully grow even more rotifers. Due to fiddling and failing to get the pump to do my bidding, I think I lost a bunch of rots, but we live and learn, and all is well right now. Once I finish the final touches, I'll post a picture or two. I may be rebuilding the table I've set the container on...
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Re:Culture Journal, Species: Brachionus plicatilis
Wednesday, March 7, 2012 8:05 PM
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Well, when one is measuring, one cannot help but notice that one is going through 40 ml of RGcomplete each day, and still the rotifers look red instead of green, a sure sign that they are not being fed enough. I had a blooming blooming culture of O. marina in with them. They were eating the algae before the rotifers could get to it. I had to filter out all my culture, and completely change the water, and I'll probably have to do it again in a few days. In the course of changing everything, and remaking my rotifer stand, as it was getting wobbly, I alternately left the air out, the heater off, and other mess ups. Many rotifers died. However, this morning, they seem active and multiplying again! They will recover. I hope it's in time for the next big nest, tomorrow. Also, while I was changing everything, I installed a valve at the bottom of the tub. Now the procedure to remove rotifers is to brush and stir the contents of the tub, and drain off the rots into a plankton filter over the bucket. Then replace the volume with clean water. Currently I am keeping 8 gallons going. Now that I'm not feeding 8 gallons of O. marina, I expect that the density of rotifers will increase, and I won't go broke feeding them.
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Re:Culture Journal, Species: Brachionus plicatilis
Wednesday, March 7, 2012 9:06 PM
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You're feeding 40ml of RGC? What size cultures? I'm doing fine feeding ~3ml, twice a day, in each 4G culture.
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