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Re:Culture Journal, Species: C- Isochrysis galbana
Tuesday, December 20, 2011 9:07 PM
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Perhaps temperature is not the problem, but I don't know that since both temperature and freshness of fertilizer are changed in my setting. Iso seems darker every day. We'll call 90 days at 1/28/2012, since that will be a continuous culture with no crashes. Then we'll have to see if I can keep the culture going over the summer of 2012.
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Re:Culture Journal, Species: C- Isochrysis galbana
Friday, December 23, 2011 4:01 PM
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Here's my beautiful golden brown:
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Re:Culture Journal, Species: C- Isochrysis galbana
Friday, December 23, 2011 4:12 PM
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Nice color. Is that before or after a split?
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Re:Culture Journal, Species: C- Isochrysis galbana
Friday, December 23, 2011 7:17 PM
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I know, right? It's a much prettier color than I remember T -Isochrysis being, but this is C-Iso...It's before the split. I know it's a little thin, but I am trying to split while the culture is in log phase. So I wait for a dramatic color intensifying change, and then I split.
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Re:Culture Journal, Species: C- Isochrysis galbana
Friday, December 23, 2011 8:00 PM
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Just FYI, that's slightly darker than my Iso cultures look *after* I split them.
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Re:Culture Journal, Species: C- Isochrysis galbana
Friday, December 23, 2011 9:24 PM
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Yeah, I have the feeling I could let them go longer, but I'm anxious to keep this batch growing, and I don't want to chance that I'll hit plateau growth phase. I'm sure my natural laziness will override this concern within a short while, however, and I'll have darker cultures to work with, soon.
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Re:Culture Journal, Species: C- Isochrysis galbana
Sunday, December 25, 2011 4:43 PM
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Actually, I've been looking at so many things with my new scope that I am not sure, but I think this is C. Iso. Not all cells are swimming, though… Click on video:
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Re:Culture Journal, Species: C- Isochrysis galbana
Monday, December 26, 2011 12:26 AM
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Looks like my Isochrysis does with my new scope, but mine is a dark brown T-Iso culture, and more dense with more cells moving, even though it was about 3 weeks old! Still, this looks like a viable Iso culture to me. Keep up the good work with the Iso! Try to see if you can get it to a darker color before splitting. Mine usually takes a good 10 days or so before it's ready to split (at least). Although Iso is not supposed to require silicates, I found that it would get dark and ready to split in fewer days -- more like 6 days or so -- if I added silicate to the medium (like I do for diatoms). If you have any sodium metasilicate, you might try that and see how it works for you.
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Re:Culture Journal, Species: C- Isochrysis galbana
Monday, December 26, 2011 7:17 AM
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Thanks Jim, I don't have any of that. Where do you get it?
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Re:Culture Journal, Species: C- Isochrysis galbana
Monday, December 26, 2011 9:48 AM
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FAF: http://florida-aqua-farms...=MICRO_MACRO_NUTRIENTS One pound is a lifetime supply for a hobbyist. Be very careful not to breathe the dust! I mix up 6.2 grams in 250 ml of RO/DI water at a time, and then put 2 ml of that solution into each 2 liter media jar when I prepare the media for diatoms, and like I said, Iso seems to like it, too. If it gets thick towards the end of the 250 ml supply, I just discard and mix up a fresh solution.
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Re:Culture Journal, Species: C- Isochrysis galbana
Friday, January 6, 2012 7:12 AM
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I split the pictured bottle yesterday, the fifth of January, 13 days after the split. It was the color of weak coffee, or strong tea. Here's videos: 40 x 100x 400x I'm not happy with the resolution at 400x. Can't seem to get any kind of focus….
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Re:Culture Journal, Species: C- Isochrysis galbana
Friday, January 6, 2012 10:21 AM
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In my experience, 400x is just about useless for what we do. I'm looking to replace my 40x objective with a 1x objective so I would have 10x, 40x and 100x magnifications. That would be much more usefull, in my opinion. By the way -- I'm backing away from adding silicate to Iso as being a good idea. Twice now, after I started adding silicate to my Iso media, I started growing green Iso. I know correlation does not prove causation, but just sayin'.....
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Re:Culture Journal, Species: C- Isochrysis galbana
Friday, January 6, 2012 1:29 PM
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Sometimes it does, though. My Iso seems to be doing better just with the new fertilizer, so I am thinking that was the problem all along.
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Re:Culture Journal, Species: C- Isochrysis galbana
Wednesday, January 11, 2012 8:51 PM
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It's dark tea again. I think I'll split tomorrow. That's 7 days this time.
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Re:Culture Journal, Species: C- Isochrysis galbana
Friday, January 13, 2012 6:50 PM
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i didn't get to it. Perhaps tomorrow. Gotta find some bottles.
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Re:Culture Journal, Species: C- Isochrysis galbana
Saturday, January 28, 2012 8:08 PM
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I did split it from the above post. Here's the 90 day culture. I just took the picture: Finally….
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Re:Culture Journal, Species: C- Isochrysis galbana
Thursday, April 5, 2012 7:16 AM
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I got lazy and didn't split the culture until it was quite far gone. Still, it looked like it was growning fine, a lovely golden brown, and then one morning, it was green! I'll check under the microscope later.
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Re:Culture Journal, Species: C- Isochrysis galbana
Thursday, February 21, 2013 9:09 PM
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Well, that old culture crashed long ago, and I'm at it again! Today I risked my life in extreme winter weather to pick up this culture from Fedex. I always ask them to hold at the FEDEX when I order live things. It's a good idea when the weather is bad, so the box isn't driven around all day in the back of an unheated truck. This time, inspired by the Hawaii video presentation, I hope to be culturing at 30ppt. My Algagen culture (Seahorse Source) came in at 40ppt, so I'm starting them out at that salinity. I need to get a new air pump. My ancient ones are so weak, I need 2 of them to power two 2-liter bottles.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: C- Isochrysis galbana
Sunday, June 9, 2013 1:34 PM
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I've been back culturing Isochrysis again, as I've found it is like magic with copepods. Pods culture very well with a light dosing of live Iso. Recently I expanded my Iso cultures to 8 gallons, so I can use one gallon a day for cultures and propagations. I'm using 1 gallon jars from Wallmart and undercabinet T5 light strips, 2 per shelf.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: C- Isochrysis galbana
Sunday, June 9, 2013 2:16 PM
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A gallon a day of Isochrysis is a LOT of phytoplankton! And, yes, it is magic, isn't it?
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