Change Page: < 12 | Showing page 2 of 2, messages 21 to 40 of 40 - powered by ASPPlayground.NET Forum Trial Version
Reports tied to this Journal
Author
|
Message
|
Re:Culture Journal, Species: Parvocalanus crassirostris
Saturday, July 27, 2013 12:39 PM
( permalink)
It's a good thing I kept that hatch. I had a couple of batches of clowns that I thought were going great, have a bad die off, I think from too many rotifers, and now I really need that experimental group. Any sign of die off, and they are going into a regular tank with rotifers and a greater volume of water, and the experiment will have to wait for another time. Meanwhile I've given them another big collection of parvo and panamensis and a few rotifers. More of them are off the wall, and either eating or dead where I can't see them. The ones I can see look robust.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
|
|
|
Re:Culture Journal, Species: Parvocalanus crassirostris
Friday, August 2, 2013 1:12 AM
( permalink)
The experiment ws a complete failure as was the two batches before it. Darn, one was a double hatch of a two large nests… I got cocky and added too much rotifers and green.
Got another batch tonight.
Parvo was staging a good comeback, then I noticed today, rotifers contaminating the culture...
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
|
|
|
Re:Culture Journal, Species: Parvocalanus crassirostris
Friday, August 2, 2013 10:14 AM
( permalink)
Culture? Singular? That sucks.
|
|
|
Re:Culture Journal, Species: Parvocalanus crassirostris
Friday, August 2, 2013 3:57 PM
( permalink)
 Originally Posted by KathyL
Parvo was staging a good comeback, then I noticed today, rotifers contaminating the culture...
Welcome to the club  I had my thriving Parvo cultures contaminated with SM rots.Started all over again only to have them contaminated by Apo!  Trying my 3rd time... 
|
|
|
Re:Culture Journal, Species: Parvocalanus crassirostris
Wednesday, August 14, 2013 10:04 PM
( permalink)
I must have been mistaken. I kept the parvo culture another week, and it bloomed. I can't find any rotifers in it. There were so many copepods of all ages in there, I put 9/10 of the culture into a sterile 10 gallon tank, half filled. with a bit of live ISO. Today they are doing well. I have a lot of pods.
I refilled the gallon jar containing the remainder of my culture with clean sterile water, and some live Iso to feed it. There are still a lot of pods in there.
Meanwhile, the "ciliate" bucket is thriving with parvo and panamensis, and few rotifers if any. I took a gallon of it, sieved at 27 microns, and looked at it under the scope before feeding it to my clownfish. Actually, there are very few ciliates in that bucket. There are copepods of all sizes, panamensis with eggs, … it all looks good, and with no water changes, only occational addition of live Iso. I keep the bucket covered with a very slow air feed.
Meanwhile my panamensis is completely overrun with rotifers. I sieved it at 150 microns and put the few copepods caught into a clean sterile jar with a little ISO . We'll see.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
|
|
|
Re:Culture Journal, Species: Parvocalanus crassirostris
Thursday, August 15, 2013 11:31 AM
( permalink)
Good news on the Parvocalanus! To rid the Apocyclops of rotifers, you can try taking a split of your cleanest (fewest rotifers) co-culture, and simply starving it. Based on my experience, I would expect the Apocyclops to outlive the rotifers in a starvation situation, but beware resting rotifer cysts.
|
|
|
Re:Culture Journal, Species: Parvocalanus crassirostris
Monday, September 2, 2013 10:47 PM
( permalink)
The 5 gallon culture in a 10 gallon tank is doing well. I've put a peristaltic pump on it to give live Iso at 2 ml per hour around the clock, and they seem to have done well over the weekend while I was at MACNA. There does seem to be lot of molts floating, so I skimmed some of them off this morning. Very robust culture. The gallon jars are also doing well, although the Iso seems to be getting darker in there. It is growing faster than the parvo can eat it.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
|
|
|
Re:Culture Journal, Species: Parvocalanus crassirostris
Saturday, September 14, 2013 1:44 PM
( permalink)
And then there is the ciliate bucket. Originally loaded with the flow thru from the parvocalanus culture, (to get rid of ciliates in the main culture), it has since been contaminated whith Apocyclops panamensis and rotifers. They were all coexisting in a 5 gallon bucket that got a little air and 30 ml or so of live Iso when I remembered it was there. Parvocalanus was not evident for a week or so, then began to "come back". Now it is the dominant species, outpacing rotifers and panamensis. I would say that once "established", these copepods are hardy and prolific.
I put an Ammonia Alert badge in the 5 gallon culture( in the 10 gallon) tank before MACNA, and it registered a little ammonia. I squeezed out a sponge filter from another tank into a bucket, let the gunk settle and poured some of the water into the parvocalanus tank. A few days later, the alert showed no ammonia, so perhaps it helped, or maybe the tank was just getting the right bacteria from the air and it matured at that time. But the tank has now "cycled", so I'll be keeping the culture in there indefinitely.
I would guess that the ciliate culture bucket has cycled as well. I've done nothing to clean it except to remove a gallon or so of critters, and replace the water, I'm guessing, every couple of weeks. All in all, I'm very pleased with this culture. Parvocalanus is not hard to culture at all, so far.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
|
|
|
Re:Culture Journal, Species: Parvocalanus crassirostris
Sunday, September 29, 2013 1:58 PM
( permalink)
All my parvocalanus cultures, from the gallon jars that I've ignored except to feed them, and 10 gallon tank that gets 5 ml live iso per hour and no other attention, and the "ciliate" bucket that started with every kind of critter in there, seem to keep on keeping on with populations of Parvocalanus crassirostris that are doing very well. I should take pictures...
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
|
|
|
Re:Culture Journal, Species: Parvocalanus crassirostris
Sunday, September 29, 2013 4:06 PM
( permalink)
Yeah, once I figured out how to keep from killing them (don't overfeed), they turn out to be very low maintenance, and will easily thrive.
|
|
|
Re:Culture Journal, Species: Parvocalanus crassirostris
Sunday, September 29, 2013 8:05 PM
( permalink)
Funny thing, I decided to take my own suggestion, and get some pictures. The Parvo in the jars got TAKEN OVER by Apocyclops, massively. The "ciliate" culture which used to have parvo, Apo, rotifers and ciliates, is now Parvo and ciliates, and the 10 gallon culture is doing fine as a stand alone Parvo culture. This is so much fun! I am continuously surprised by what happens.
I am having issues with Photobucket, in that videos seem to take forever to upload, and videos are the best way to see the difference between Parvo and Apo. When I figure it out, i'll post up pix.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
|
|
|
Re:Culture Journal, Species: Parvocalanus crassirostris
Sunday, October 6, 2013 4:13 PM
( permalink)
finally, I was able to upload the video that proves I still have the culture going: 
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
|
|
|
Re:Culture Journal, Species: Parvocalanus crassirostris
Thursday, November 14, 2013 3:42 AM
( permalink)
so if ciliates creep up in your parvo, are you sieving them out or just letting them be now?
|
|
|
Re:Culture Journal, Species: Parvocalanus crassirostris
Thursday, November 14, 2013 8:00 AM
( permalink)
I haven't been doing anything to rid the culture of ciliates. The parvo doesn't seem to mind. I suppose you could sieve them out, repeatedly over many weeks. Doesn't seem worth the effort to me.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
|
|
|
Re:Culture Journal, Species: Parvocalanus crassirostris
Thursday, November 14, 2013 9:25 AM
( permalink)
hmmm. ok, thanks.
|
|
|
Re:Culture Journal, Species: Parvocalanus crassirostris
Thursday, November 14, 2013 9:49 PM
( permalink)
Kathy, you could consider a continuous ciliate remover using an airlift tube with a small sized screen on the inlet, and an even smaller sized screen or bag at the outlet. In other words, a 40 um screen on the inlet would allow only ciliates to pass into the airlift, and then a 10 um bag at the outflow would capture ciliates. Every day or two you could replace the capture bag.
This may be too much to set up. When my tanks are ready I will have large parvo cultures and will need to knock back ciliate populations on a continuous basis. This may not be worth setting up on a smaller system. The inlet screen will have a tendency to clog easily because it is so fine.
Just a suggestion.
|
|
|
Re:Culture Journal, Species: Parvocalanus crassirostris
Monday, March 31, 2014 8:59 AM
( permalink)
I actually like having ciliates in the ciliate bucket. I don't know if it was the ciliates or the parvocalanus that allowed me to raise Corythoichthys intestinalis to 6.5 weeks…perhaps both.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
|
|
|
Re:Culture Journal, Species: Parvocalanus crassirostris
Monday, March 31, 2014 9:07 AM
( permalink)
You certainly could be right. Parvo naups are only twice the length of many ciliates, so they are probably only 4 times the food volume. A larvae that hunts well could be growing on ciliates just as easily as on parvo (or in combination).
It just surprised me that your parvo cultures weren't taken over by ciliates given the much faster reproduction rates of the ciliates. You are certainly doing something right.
|
|
|
Re:Culture Journal, Species: Parvocalanus crassirostris
Monday, March 31, 2014 9:31 AM
( permalink)
Perhaps the spare feeding they get keeps the ciliates from taking over in that bucket. I've done very little to keep it going, since I have better cultures of parvo elsewhere.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
|
|
|
Re:Culture Journal, Species: Parvocalanus crassirostris
Saturday, May 10, 2014 3:33 PM
( permalink)
It always amazes me how much I can learn about a thing when I'm trying to learn about something else entirely.
The kreisel I set up to make a stab at raising damsels turns out to be an ideal P. crassirostris culture vessel. Too bad I didn't take better notes. Over a weeks' time I set it up over a mild heat source, added P. c. and live Iso (just a tint) and a bazillion damselfish eggs. The P.c. did very well, the Iso got really almost tea colored, the damsel eggs hatched and lasted until starvation day3, whereupon they all died. I removed some of the dead bodies but mostly left them in there. P.c. continued to bloom and I needed no additions of Iso. At the end of 6 days or so, I added just a few damsel eggs and watched as the Iso got thinner and the water more clear. I added more Iso as needed. The pods are so numerous that I sucked out all the ones I could in 500ml of water, that were crowding the side closest to the light. The concentration in 500 ml was 10 pods per ml. All long antennaes, so they must be P.c. I did not see any Apocyclops at all. But they are zooming around the kreisel like I've never seen Parvo do before. I was sure I had an Apo contamination. Nope.
Even after harvesting these, the concentration looks unchanged. I have to make the water light tea colored EVERY DAY, as it clears that fast.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
|
|
|
|