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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Tuesday, April 15, 2014 7:25 PM
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I pulled the nest today around 2 PM and some start hatching in the bowl right away. I wonder if these start hatching out during the day naturally. In any case, I have them in a giant wine glass type thing I got from Walmart that is about 1.5 gallons. I added some gymno but I think there is also some tetraselmis in at least one of the gymno cultures. I hope to add H. triquetra in a couple days as well once those cultures come back up since I just split them.
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Wednesday, May 21, 2014 4:47 PM
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On Monday I pulled a nest thinking they would hatch that night. They started to hatch the following night (5-20) instead. I put the eggs in a BRT with broodstock water and aerated them with an airstone. I added Heterocapsa triquetra (dinoflagellate) as a potential food. This morning I saw a few larvae swimming around, but not as many as I had expected. There are still many unhatched eggs, and I am sure there are lots of larvae I can't see due to how small they are and because they are black--same color as the tank. There is a bunch of gunk on the bottom, likely from whatever settled out of broodstock water. I probably should have sieved it first, but was a bit rushed. I may do a small bottom siphon tomorrow.
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Thursday, May 22, 2014 10:41 PM
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Well, this evening I couldn't spot a single larvae. I don't think it was a great hatch. I will attempt the Heterocapsa for food again since I can't yet rule it out--the problems from this last attempt weren't likely food related. Next time I will go back to hatching them in a smaller container and won't use an airstone. I think I will also use more diffuse light. This last time the lights may have been too bright. Before I would tint the water with phyto, but the Heterocapsa doesn't grow that dense/dark that it would work for brown water. Luckily these guys spawn frequently
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Monday, May 26, 2014 9:09 AM
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I pulled the nest for hatch last night. They had spawned on the half clam shell this time since I hadn't returned their spawning PVC to them in time. I put the clam shell in a 1 gallon fish bowl and put an airstone near it, turned down pretty low. There were just enough bubbles to slightly move the eggs. They started to hatch right away, under bright light. I got a short video of the hatch that I will try to upload/post tomorrow. I let about half of the nest hatch in the fishbowl, then I put the shell back with the parents. There are still A LOT of larvae. Hundreds of them. Before I put the eggs in the bowl, I added Heterocapsa dinoflagellates. I covered the sides with a hand towel and put a sheet of paper towel over top to dim the light a bit. This morning I added some Oxyrrhis marina, a drop of RGOmega and a drop of Prime. I also took the paper towel off for the day. Still plenty of larvae.
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Monday, May 26, 2014 11:32 AM
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Shannon, I'm just pulling at straws here and speculating, so please take this with a grain of salt. If the larvae survive until around day 4 it might indicate that (even though they are eating) dinos and tetraselmis may not be an appropriate diet at this stage and this is just the point that the yolk sack is depleted. The parvo naup (which I believe is around 60 um) looked a little on the big side so 30-40 um ciliates might be an appropriate food. When you write that you sieved several miscellaneous cultures for 27 micron to 53 micron food, do you think you harvested much? Did the batches fed ciliates live as long as the dino batches? While common sense says to add every type of food you have so the larvae can choose, you may also want to culture something like euglena or euplotes to see if that size works on the larvae. I think carolina supply has both of those. Also, this is getting a little farfetched, but you might mix an algae like pavlova with the ciliates for better digestibility. Perhaps none of this will help and will just be a lot more culture work. In that case, sorry if it doesn't help.
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Monday, May 26, 2014 5:19 PM
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I agree tetraelmis is not a good option, but they do actually eat it. The longest survival I got was day 5 or six and that was when I added some nauplii that I ordered (that were already too large, I would imagine). I never confirmed if they were eating or not with that run. There is a paper, by Wittenrich and others, about another species in this genus. They were fed wild plankton, yet the only thing they ate were gymnodinium dinoflagellates. That is why I want to give a few attempts where I try that, at least for the first week or something. The starvation trials also had them lasting until day 5, which makes me think that my deaths may be more environmental than food related at this point. I am working with a different species though, and I can't say that they are going to be the same. So I want to try the dinos for a first food, then try Parvo. The Parvo just isn't ready to use yet. I also want to try Oxyrrhis marina and ciliates.
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Monday, May 26, 2014 5:57 PM
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I just pulled two larvae and got these pics Looks like they are at least eating the Heterocapsa
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Monday, May 26, 2014 7:24 PM
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It looks like you are making good headway. Very interesting that they are taking dinos over ciliates given that ciliates probably move more and would elicit more of a strike response. But you can't argue with results!
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Tuesday, May 27, 2014 7:24 PM
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Well, who knows if it will sustain them, but they seem to be ingesting more than just incidentally. Today I added some Euterpina, 53 to 180 microns. I still don't feel ready to feed Parvo, which would be more ideal. Or perhaps the Colurella rotifers I am trying to get going. But not quite there yet...
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Wednesday, May 28, 2014 5:19 PM
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Sunday, October 5, 2014 8:42 PM
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Any news with these guys? Was considering getting a pair soon.
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Re: Breeding Journal, Species: Priolepis hipoliti (Rusy Goby)
Wednesday, October 8, 2014 7:42 PM
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Nope. I haven't attempted for awhile. I fed less this summer so they didn't lay for awhile, but now I'm feeding more again and hope to give them a go again soon.
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