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Re: Breeding Journal, Species: Synchiropus Ruby Red sp.
Saturday, August 15, 2015 5:25 AM
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The later(lots of P. crassirostris). Particularly since I didn't do the former (copepod salad) this time around. There may be some ciliates in there, but all I'm intentionally feeding them is parvo and live phytoplankton.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re: Breeding Journal, Species: Synchiropus Ruby Red sp.
Saturday, August 15, 2015 9:12 PM
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Days 9 and 10, still going well though perhaps some die off. I have to remove adult parvo from the larval jar and add back nauplii, although I am at 12 naups/eggs per ml now. From a 6 liter jar, I am changing 2 liters a day. Soon they should be eating copepodites, right?
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re: Breeding Journal, Species: Synchiropus Ruby Red sp.
Sunday, August 16, 2015 11:02 PM
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9-10 days - keep up the great work Kathy!
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Re: Breeding Journal, Species: Synchiropus Ruby Red sp.
Monday, August 17, 2015 3:15 PM
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Now days 12 and 13. Some die off, way too many parvo nauplii (50 / ml). I can't say they are growing, but I look at them constantly, so growth is hard to judge. Parvo are not rotifers. They don't just multiply, they spawn eggs and nauplii that grow in a day and aren't nauplii any more, so if the larvae eat only nauplii, they are going to need more new ones each day. I now have the happy problem of too many parvo. I started this run not having enough. I should give a MBI workshop talk on Parvo. It would bore everyone to tears.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re: Breeding Journal, Species: Synchiropus Ruby Red sp.
Monday, August 17, 2015 5:07 PM
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Anxious to see a current pic, but I do understand if you don't want to traumatize them with a photo session. How many larvae do you estimate are still alive? And 50 naups per ml sounds awesome - they should not be lacking for food! Does your pretzel jar have any airline or other circulation
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Re: Breeding Journal, Species: Synchiropus Ruby Red sp.
Monday, August 17, 2015 6:12 PM
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Kathy I'm pretty sure a talk dedicated to high density Parvo culture wouldn't bore any of us to tears lol
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Re: Breeding Journal, Species: Synchiropus Ruby Red sp.
Monday, August 17, 2015 9:21 PM
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Originally Posted by JoeDigiorgio
Kathy I'm pretty sure a talk dedicated to high density Parvo culture wouldn't bore any of us to tears lol I'm pretty sure it would. It involves a lot of math and biology: things normal people avoid learning. Clearly, despite near constant surveillance, I've bungled the thing. Major die off today, day 12 and 13. This evening I am doing a 70% water change to remove the amazing bloom of parvo. and leave a more reasonable concentration of food for them. I hope it doesn't kill them all. We are almost at 2 weeks. No measurable ammonia, but these guys may be hyper sensitive, and from the papers I've been reading, parvo hatching into nauplii seems to generate ammonia more so than the adult copepods do by themselves. I don't think we can stand much more in the way of losses, or we will be down to zero larvae.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re: Breeding Journal, Species: Synchiropus Ruby Red sp.
Monday, August 17, 2015 9:30 PM
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Originally Posted by Lrood
How many larvae do you estimate are still alive? Looks like maybe 10. Started with a couple hundred, estimated.
Originally Posted by Lrood
And 50 naups per ml sounds awesome - they should not be lacking for food! They are certainly not lacking for food, and I'm happy that I can culture parvo to get that kind of number, but how would you feel if you were being pelted by 10 times as many donuts as you could possibly eat, constantly? Too much food is likely to be a bad thing too.
Originally Posted by Lrood
Does your pretzel jar have any airline or other circulation Yes, it has a rigid open airline with just enough air to make a vertical circulation, like a kreisel effect. As much as I am tickled to have gotten them to almost 2 weeks in a pretzel jar, if I have the opportunity to do this again, I'll probably use a BRT. I would like to try the pretzel jar with damsels again, though I don't think any survived this time around. I like that I can see what's going on. In a BRT, so often, you add larvae or eggs, and hope. Seeing them is not easily accomplished.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re: Breeding Journal, Species: Synchiropus Ruby Red sp.
Tuesday, August 18, 2015 8:55 AM
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day 13 and 14 and only 4 or 5 left, and they are not looking too good. 20 nauplii per ml.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re: Breeding Journal, Species: Synchiropus Ruby Red sp.
Tuesday, August 18, 2015 11:41 PM
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Did another massive water change and got them down to 8 nauplii per ml. Remaining few larvae are actively hunting instead of drifting around their watery ferris wheel. Not holding up too much hope, though.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re: Breeding Journal, Species: Synchiropus Ruby Red sp.
Wednesday, August 19, 2015 7:31 AM
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Originally Posted by Lrood
Anxious to see a current pic, but I do understand if you don't want to traumatize them with a photo session. How many larvae do you estimate are still alive? And 50 naups per ml sounds awesome - they should not be lacking for food! Does your pretzel jar have any airline or other circulation This was day 11 or 12: Day 14 or 15, almost killed this one, member of the last 3 or so larvae.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re: Breeding Journal, Species: Synchiropus Ruby Red sp.
Wednesday, August 19, 2015 7:32 AM
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they don't look a lot different.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re: Breeding Journal, Species: Synchiropus Ruby Red sp.
Wednesday, August 19, 2015 9:44 PM
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Can't find any this evening.Day 14 and 15. Disappointment moderated by first spawn of G. okinawae pair!
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re: Breeding Journal, Species: Synchiropus Ruby Red sp.
Friday, August 21, 2015 12:31 AM
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What if you tried adding a second food? I remember Witt telling me that Pseudodiamptomus was useful for Synchiropus culture.
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Re: Breeding Journal, Species: Synchiropus Ruby Red sp.
Friday, August 21, 2015 9:00 AM
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Not a bad suggestion. I really think they were killed by the pelting donuts of Parvo overpopulation. Working on it….
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re: Breeding Journal, Species: Synchiropus Ruby Red sp.
Friday, August 21, 2015 9:07 AM
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Spoke too soon. Last night I did find one surviving on neglect. I fed some phyto, and keep the heat, air and lights on, but I stopped trying to control copepod population otherwise. I don't expect much, but raising one is not a bad thing.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re: Breeding Journal, Species: Synchiropus Ruby Red sp.
Friday, August 21, 2015 10:35 AM
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These are getting 24hr lighting?
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Re: Breeding Journal, Species: Synchiropus Ruby Red sp.
Friday, August 21, 2015 12:04 PM
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yes.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re: Breeding Journal, Species: Synchiropus Ruby Red sp.
Friday, August 21, 2015 5:51 PM
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What do you think about maybe 4-6 hours of darkness a night? My understanding has always been that 24hr lighting keeps the larvae eating all the time, with the idea of increased intake thereby making up for possibly underwhelming nutritional density. With the prey available to your larvae, maybe periods of rest could help? To run with Matt's comment regarding a second prey item, Isochrysis is tauted as the holy grail of algae and the same for Parvo, but we forget that Iso isn't necessarily nutritionally balanced, and its all we feed parvo. Perhaps the high DHA content and relatively low EPA is holding development back during a crucial time. Days 12-15 should be a time of high growth and development if these model other scooters, settling at days 18-21. Maybe bringing in a second copepod, fed a different algae, is the answer.
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Re: Breeding Journal, Species: Synchiropus Ruby Red sp.
Friday, August 21, 2015 6:23 PM
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You can add a diatom to the diet, like Thalassiosira pseudonana or T. weissflogii, and achieve higher EPA levels when fed to the Parvos. T.p. or T.w. have ample EPA content of roughly 20% of fatty acids. In addition to Iso, I always feed my Parvos, and other pods, a diatom to achieve a more balanced diet. I'm a huge fan of offering a binary, or trinary, diet for any live feed organism I'm working with. Chad
<message edited by clayton447 on Friday, August 21, 2015 7:00 PM>
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