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Culture Journal, Species: Moina salina
Monday, August 22, 2011 9:45 PM
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Culturing Journal DataSheet This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Moina salina Species description: Saltwater daphnia Culture source (link if possible ): Algagen, Jim Welsh If algae, CCMP # (Optional ): http://ccmp.bigelow.edu/ Culture Establishment Date: June 8, 2011 Continuation Date: 11/21/2011 Culturing Vessel Details Salinity: 20 ppt Temperature: 78F pH: 8 Vessel description: Indoors: gallon jar . Outdoors: 17 gallon black round tub outdoors on concrete patio, shaded from afternoon sun by a large sycamore tree. Bird netting secured over top to keep out birds, squirrels and small children (drowning hazard). Lighting description: Indoors: fluorescent Grow light, 5500 K . Outdoors: Natural sunlight, though mostly shaded Lighting cycle: Indoors: 14 day/ 10 night. Outdoors: as varied as the seasons Aeration description: Indoors:rapid, but not boiling, with open airline to bottom of jar, air pump. ,/ sometimes just a cap with a hole in it, and no pumped air Outdoors: as the wind blows. Methodologies Split methodology: Indoor culture: When sediment accumulates, turn off air for an hour or so, and decant culture into clean jar. Sometimes filtered the daphnia to clear the medium, and backwashed into clean saltwater. Outdoor culture: No care whatsoever. Culture medium description: Indoor: Algal food is 6 parts N-rich, 1 part Roti-Grow+, equal volume ChloramX. Fed this, about 12 drops, twice a day. Also fed daily about 100 ml Oxyrrhis marina, fed with same algal food. Sometimes fed with RGcomplete. Outdoor: Aged saltwater in 17 gallon black round tub outdoors on concrete patio, layer of detritus on bottom from pollen, insects, small leaves, etc. that fell thru the bird netting. I noticed an occasional algal bloom. Once I dumped some wild algae in from my attempts at culturing. Otherwise no intentional feeding. Cell count: not known. Outdoor culture bloomed during the summer with M. salina--very dense. Lasted about 10 days. Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread. If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by KathyL on Monday, November 21, 2011 9:30 AM>
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Re:Culture Journal, Species: Moina salina
Tuesday, August 23, 2011 6:01 AM
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Original culture from Jim Welsh. These did very well for me producing a pretty dense culture for 3 weeks to a month, and then appeared to crash. I had not done much about their water quality, little to no water changes, I don't remember, and didn't write anything down. I suspect their crash was due to water quality. I was able to get a few survivors and restarted the culture. By a few, I mean I counted 4. In a week's time there were many more. Either they are very prolific or I mis-counted the survivors. The literature suggests that they drop their eggs, but are capable of parthenogenesis as well. My current practice is to feed them twice daily with 6pNR/1RG+, and once daily with a pudding cup's worth of my O. marina culture. When the sediment accumulates and the water looks cloudy with detritus, I turn off the air for an hour or so to let the culture settle, and then decant the culture into a clean jar. One should not do this too frequently, however, as one will likely lose a crop of eggs that fall to the sediment. I suppose one could add clean saltwater to the sediment and wait to see if the eggs hatch and nauplii swim to the water column and then decant/harvest the nauplii. I'll try this next time.
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Re:Culture Journal, Species: Moina salina
Tuesday, August 23, 2011 3:57 PM
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Didn't AlgeGen get their culture from Angi?
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Re:Culture Journal, Species: Moina salina
Tuesday, August 23, 2011 3:59 PM
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Who's Angi? I have no idea….
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Re:Culture Journal, Species: Moina salina
Tuesday, August 23, 2011 4:32 PM
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Re:Culture Journal, Species: Moina salina
Tuesday, August 23, 2011 4:40 PM
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 Originally Posted by GreshamH
Didn't AlgeGen get their culture from Angi? Yes. Basically, I kept harassing and harassing Angi until she agreed to ship me some Moina salina. I also told her about the demand here in the US for Moina, so she decided to send Eric some at the same time she sent me mine, "so there will be a permanent source in the US", as she put it. That's how it came to be here in the US, most recently. If there was another source for it in the US before that, nobody on MOFIB or Seahorse.org could locate any. All leads turned out to be just freshwater Daphnia.
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Re:Culture Journal, Species: Moina salina
Tuesday, August 23, 2011 6:07 PM
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 Originally Posted by GreshamH
Hmm, just spent the last half hour looking up Angi and me. You think that I can remember one exchange I had through a translation with a person in a thread about Rhodomonas from two years ago? Really? I have lots of excuses for having a bad memory: - Post-menopausal,
- taking medication that prevents sleep,
- long life of cramming stuff in my brain... There is simply no more room in there!!!!
Gresham, please find it in your heart to give an old woman a break, will ya, pal?
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Re:Culture Journal, Species: Moina salina
Tuesday, August 23, 2011 6:12 PM
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Well I never had any exchanges with her I don't think.. I just pick up on facts like that
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Re:Culture Journal, Species: Moina salina
Tuesday, August 23, 2011 6:13 PM
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And here's my culture shelf: I like these square jars. They take up less space than round ones, and decanting is easy. Wide mouth makes cleaning easy. Hand holds make handling easy. WalMart. Cheap. M. salina is on the left. I gotta get some more square jars...
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Re:Culture Journal, Species: Moina salina
Tuesday, August 23, 2011 6:16 PM
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@ Gresham, no worries, bro.
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Re:Culture Journal, Species: Moina salina
Friday, August 26, 2011 5:15 AM
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M. salina is definately more dense this week. I am seeing lots of larger white twitchers in the culture. Feeding twice a day now. Just barely tinting the water. It does look a bit cloudy. I am not sure if there is bacteria, or if the O marina is causing the cloudiness. I'll renew the culture on the weekend.
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Re:Culture Journal, Species: Moina salina
Sunday, September 4, 2011 3:41 PM
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This culture has done really well, considering that I had only about 4 individuals when I restarted the culture. I have one gallon of a pretty dense culture, considering that its a copepod.
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Re:Culture Journal, Species: Moina salina
Sunday, September 4, 2011 5:37 PM
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*AHEM* Moina salina is NOT a copepod. I know Algagen sells them as part of thier "Reef Pods" line, which includes a bunch of different copepods and, then, also Monia salina. It also doesn't help any that Vince Rado misdentified Moina salina as a copepod in the caption of one of the images in his recent article in Reef Hobbyist Magazine (and the image isn't even of Moina!). But still, Moina is a Cladoceran, in a different Class (Cladocera) from copepods, which are in the Class Maxillopoda. They are all crustaceans, but the similarity ends there, taxonomically. Just had to get that off my chest! Aside from that, good work, Kathy, bringing them back to such numbers starting with so few!
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Re:Culture Journal, Species: Moina salina
Sunday, September 4, 2011 6:49 PM
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It's good we have you here to keep us accurate! New word for Kathy: Cladoceran.
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Re:Culture Journal, Species: Moina salina
Sunday, September 4, 2011 6:49 PM
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Good for you, Kathy. Mine never came back.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Culture Journal, Species: Moina salina
Wednesday, September 28, 2011 7:12 AM
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really dense culture now. Water is clear after 8 hours, I am feeding my gallon jar about 100 ml O. marina once a day, and 12 drops of Andy's mix twice a day, with about the same amount of 60g/liter chloramX. Don't need a microscope to see the swimmers. Click the vid:
<message edited by KathyL on Wednesday, September 28, 2011 10:34 AM>
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Re:Culture Journal, Species: Moina salina
Friday, September 30, 2011 5:34 AM
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One of my outdoor tubs that used to be dense with rotifers, and pretty dense with A. panamensis, is now not so dense with rotifers or panamensis, and chock ful of M. salina.
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Re:Culture Journal, Species: Moina salina
Sunday, October 16, 2011 2:33 PM
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My "clean" indoor culture of M. salina crashed very quickly. I think that ammonia may be the culprit, but really it is inattention by me. I did not change the jar on schedule. Interestingly, the jar, which I regularly supply with O. marina, had no O. marina in it, just lots of rotifers and ciliates. They did not seem to care about the ammonia. Fortunately, the outdoor tub is chock full of M. salina, even as the weather has cooled significantly. I'm using some of these daphnia to restart my indoor culture. I found a couple of Tigriopus californicus in there, but I don't mind as long as the M. salina survive.
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Re:Culture Journal, Species: Moina salina
Monday, November 21, 2011 6:30 AM
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I had two jars that I took from my outdoor culture. One when the culture was dominated by M. salina, and then a few weeks later, after a cool spell, one that was dominated by T. californicus. The M. salina did well, with air and food, and I decanted the jar a few times, but the last time, I kept the sediment and water (about an inch deep in the jar), just set it on the shelf. When I collected a jar of the T. californicus, I also set it on the shelf, opposite end. Weeks later, I notice that the T. californicus jar has a nice population of M. salina in it,click on video: and the M. salina jar has a nice population of T. californicus. I'm going to have to find a mesh size that keeps the M. salina and allows T. californicus to pass thru. The sediment jar bloomed with something, so I poured it into a plastic tray with a lid, and a hole in the lid, and that culture continues to do well with sparse feeding, just sitting on the shelf with no further aeration. Thankfully, the A. panamensis culture appears to be uncontaminated.
<message edited by KathyL on Monday, November 21, 2011 9:34 AM>
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Re:Culture Journal, Species: Moina salina
Monday, November 21, 2011 9:39 AM
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Here is a microscope shot of the M. salina in the T. californicus jar: I took a pudding cups' worth, about 90 ml, and filtered and backwashed into a small dish to concentrate them. Click on the video, 40x thru the scope: I have both T. californicus and M. salina in there.
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