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•  Phytoplankton Culture

  Change Page: < 1234 > | Showing page 2 of 4, messages 21 to 40 of 67 - powered by ASPPlayground.NET Forum Trial Version Author Message EasterEggs Total Posts : 1946 Scores: 19 Reward points : 735 Joined: 9/22/2011 Location: Saskatoon, SK, CA Status: offline MBI: 590 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Phytoplankton Culture Friday, November 4, 2011 8:25 AM (permalink) 0 Originally Posted by JimWelsh Get Algagen starters from SeahorseSource.com:  http://www.seahorsesource...;category=Foods-Algaes You might be able to make adequate media with your chemicals, but Guillard's F/2 (Micro Algae Grow) is a proven medium that includes vitamins and trace minerals, and is very inexpensive.   The trouble is duties and shipping Jim.  I need to order starter from SeahorseSource, fert from FAF, I need some bigger micron sieves (nesting set from BSD?)...ack, one thing from 3 different places.  Argh.  My last order from FAF was $90 in products and $65 in shipping and duties.     I can order AlgaGen and Reed products through my LFS though, so I try to get whatever I can from those two sources.   #21 Fishtal Total Posts : 5467 Scores: 37 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Phytoplankton Culture Friday, November 4, 2011 10:40 AM (permalink) 0 For the sieves, just buy the mesh and make your own. http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #22 luis a m Total Posts : 451 Scores: 2 Reward points : 1249 Joined: 3/15/2011 Location: Buenos Aires, Buenos Aires, AR Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Phytoplankton Culture Friday, November 4, 2011 12:50 PM (permalink) 0 And you can make Guillard with your NO3 and PO4 plus some aquarium trace and vitamins.   #23 EasterEggs Total Posts : 1946 Scores: 19 Reward points : 735 Joined: 9/22/2011 Location: Saskatoon, SK, CA Status: offline MBI: 590 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Phytoplankton Culture Friday, November 4, 2011 7:55 PM (permalink) 0 Originally Posted by luis a m And you can make Guillard with your NO3 and PO4 plus some aquarium trace and vitamins.   Ya?  I will look into this more.  It would be nice to use what I already have.  The Plantex CSM+B is a trace mix:   Total magnesium - water soluble chelated magnesium                1.5% Copper - chelated                                                                    0.1% Iron - chelated                                                                          7.0% Manganese - chelated                                                              2.0% Molybdenum                                                                           0.06% Zinc - chelated                                                                         0.40% Boron                                                                                      0.8% EDTA - minimum content                                                        55%   Originally Posted by Fishtal For the sieves, just buy the mesh and make your own.   I have been considering this.  Order mesh from Amazon maybe...?   #24 THEJRC Total Posts : 1006 Scores: 4 Reward points : 555 Joined: 10/23/2009 Location: Colorado Springs, CO, US Status: offline MBI: 210 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Phytoplankton Culture Friday, November 4, 2011 8:03 PM (permalink) 0 he he...   https://ncma.bigelow.org/node/58   more suitably:   https://ncma.bigelow.org/node/79   I was playing with K medium for a while as the lower trace metal concentrations really lends itself to a cleaner more useable medium in regards to larviculture   https://ncma.bigelow.org/node/81   Pelagically yours, ~J         #25 EasterEggs Total Posts : 1946 Scores: 19 Reward points : 735 Joined: 9/22/2011 Location: Saskatoon, SK, CA Status: offline MBI: 590 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Phytoplankton Culture Saturday, November 5, 2011 11:55 AM (permalink) 0 Awesome, thanks Joe!  Those are not the exact same chemicals I have though dangit.  My chems are KH2PO4 (0-51-34), KNO3 (13.5-0-46.2), K2SO4 (0-0-50).  The chems listed in the f/2 recipe are all sodium based, not potassium based...I bet that makes a big difference.  I also notice they are mixing the recipes with natural seawater, not artificial seawater. Oh, and holy wowzers NCMA sure has a lot of strains available for purchase!  A little pricey though...   #26 luis a m Total Posts : 451 Scores: 2 Reward points : 1249 Joined: 3/15/2011 Location: Buenos Aires, Buenos Aires, AR Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Phytoplankton Culture Sunday, November 6, 2011 12:23 AM (permalink) 0 Mindy,you only need to buy sodium nitrate and phosphate,weigh 75 and 5 grams and make the stock solutions in destiled water.Piece of cake...   #27 THEJRC Total Posts : 1006 Scores: 4 Reward points : 555 Joined: 10/23/2009 Location: Colorado Springs, CO, US Status: offline MBI: 210 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Phytoplankton Culture Sunday, November 6, 2011 3:46 PM (permalink) 0 lol I dont buy the average alga from CCMP... errr now NCMA lol....  just the oddballs I like to play with   Plankton is sorta my thing...  as far as the water mixture I have not had a problem using most of the formulations with artificial seawater, in fact the only medium I've had issue with lately is with those provided by carolina biological supply, they simply hate artificial SW!!   The 75/5 recipe that Louis mentions also works well and it's actually an FAO guideline if your using sodium dihydrogen orthophosphate.  Bear in mind the Guillards formulation is meant to be very flexible and generic and may contain more trace than needed for many species (a lot of formulations are like this).   if formulating your own your going to find a fun experimental course... things like sodium metasilicate, Iron chloride, maganese chloride..... eh the list goes on... always fun!!  Just be ready to experiment and change formulations based on species. Pelagically yours, ~J         #28 EasterEggs Total Posts : 1946 Scores: 19 Reward points : 735 Joined: 9/22/2011 Location: Saskatoon, SK, CA Status: offline MBI: 590 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Phytoplankton Culture Wednesday, November 9, 2011 10:23 AM (permalink) 0 Hmm...maybe I'll just buy the Guillard's.  LOL.  I don't know where to buy these other chems from.  I just figured it would be as simple as mixing up some saltwater and adding some N, P, and K.  Apparently not...   Oh, and sorry I was AFK for so long without warning...puter got a REAL GOOD virus this time!   #29 JimWelsh Total Posts : 1426 Scores: 14 Reward points : 1486 Joined: 1/22/2010 Location: Angwin, CA, US Status: offline MBI: 1845 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Phytoplankton Culture Wednesday, November 9, 2011 10:44 AM (permalink) 0 Sigma-Aldrich is one place to get the chemicals from, and you can get some very pure materials, but they are a bit pricey:  http://www.sigmaaldrich.c...=RS&N25=0&F=PR   I do wonder what quality/purity/grade you might need when making your own media, since having high level of some trace metals seems to matter.    Since there are so many different DIY projects we can throw ourselves into in this hobby, I submit that experimentation with phyto media should be very low on the list!  I prefer to just buy Micro Algae Grow and be done with it.  It works.  Phyto culture is difficult enough for most people that I don't think that uncertainty about whether or not a DIY media is a source of potential problems should be introduced into the mix, IMHO.     #30 KathyL Total Posts : 2639 Scores: 13 Reward points : 1504 Joined: 6/6/2010 Location: St. Louis, MO, US Status: offline MBI: 1605 Pnts MBI Site: Marine Aquarium Societies of North America Re:Phytoplankton Culture Wednesday, November 9, 2011 12:55 PM (permalink) 0 I'm with Jim.  I live in Sigma's home town, and its cheaper to buy stuff from FAF.  Also, a lot less work.   #31 GreshamH Total Posts : 757 Scores: 16 Reward points : 600 Joined: 4/27/2011 Location: SF Bay Area, CA Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Phytoplankton Culture Wednesday, November 9, 2011 5:30 PM (permalink) 0 Chemicals are best bought with major shopping.  Buying all from one supply house is not recommended unless you have bottomless pockets   Don't limit yourself to the major chemical houses either.   #32 THEJRC Total Posts : 1006 Scores: 4 Reward points : 555 Joined: 10/23/2009 Location: Colorado Springs, CO, US Status: offline MBI: 210 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Phytoplankton Culture Thursday, November 10, 2011 7:51 AM (permalink) 0 Originally Posted by GreshamH Chemicals are best bought with major shopping.  Buying all from one supply house is not recommended unless you have bottomless pockets Don't limit yourself to the major chemical houses either.   I second that!  But much like Jim Welsh pointed out, this hobby is full of DIY possibilities we can spend our time and dough on.  As with everything theres a ton of research, trial and error, and just plain time involved.  Buying prepared medium from FAF just makes sense.  That said I would check around locally and see if there are others interested, the Mass Packs are very cost effective  and you can easily split an order with multiple people (not sure how much medium you go through).   Then again, it's always good to realize that algae is not an american deal   there are algae banks all over the world you can hit up   http://www.phycol.ca/   Pelagically yours, ~J         #33 EasterEggs Total Posts : 1946 Scores: 19 Reward points : 735 Joined: 9/22/2011 Location: Saskatoon, SK, CA Status: offline MBI: 590 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Phytoplankton Culture Thursday, November 10, 2011 9:05 AM (permalink) 0 Thanks for the link Joe.  I didn't realize there were all these "phyco farms" around, nor did I know that this type of place would sell starter cultures to any ole person.  That particular place does have Iso, but not Nanno.  Which is ok because Iso seems to be the tough one to get anyway.  Their prices are the highest I have seen yet, but it would cost me that much in shipping to buy from the US anyway so I guess it doesn't really matter.  Apparently Canadian algae is more lucrative.    Regarding the DIY fertilizer, I think you guys are right.  I didn't realize it would be so in-depth to mix the ferts so I will go ahead and buy the ferts from FAF.  They can be sent USPS so that won't be too bad for shipping.  When things have to be sent via courier that's when shipping gets pricey...brokerage fees are ridiculous (for UPS in particular).   #34 THEJRC Total Posts : 1006 Scores: 4 Reward points : 555 Joined: 10/23/2009 Location: Colorado Springs, CO, US Status: offline MBI: 210 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Phytoplankton Culture Saturday, November 12, 2011 9:15 AM (permalink) 0 google around for "algae banks" the link I posted was just the first thing that popped up under "canada algae bank".  There are tons of sources worldwide for many of the species we hobbyists want (iso, nanno, dunaliela, etc.) you'd be amazed what you can find when you break down the other uses and track along those lines.  i.e. biofuel hobbyists, university biology supplies, and so on.    Algae banks are typically going to be your most expensive sources but also your most isolated and "axenic" cultures (very clean).  the biofuel sources are going to range in accuracy and cleanliness depending on how dedicated the supplier is (much like the traditional aquaculture / reef hobbyist sources).   I tend to only go to the algae banks for odd strains that arent too common, everything else typically comes from FAF or carolina biological supply as both sources are low cost here in the US.   Important to have your ducks in a row though before you order the algae itself, especially if your paying a premium to an algae bank or for customs.  I've gotten impatient and stupid more than once and lost a culture due to it showing up prior to me having my culture rack ready.  Scrambling last minute is always a bad thing! Pelagically yours, ~J         #35 EasterEggs Total Posts : 1946 Scores: 19 Reward points : 735 Joined: 9/22/2011 Location: Saskatoon, SK, CA Status: offline MBI: 590 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Phytoplankton Culture Saturday, November 12, 2011 9:45 AM (permalink) 0 Thanks Joe, I do have to set up my rack first!  Maybe today or tomorrow....?   #36 EasterEggs Total Posts : 1946 Scores: 19 Reward points : 735 Joined: 9/22/2011 Location: Saskatoon, SK, CA Status: offline MBI: 590 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Phytoplankton Culture Thursday, March 22, 2012 8:57 PM (permalink) 0 Could some of you please comment on temperature?  I see "temperature tolerant" referred to sometimes, but what is the preferred temperatures for Nanno, Tet, C-Iso, and T-Iso?   Fwiw, Googling found a fert recipe of 2 liter saltwater, 5 mL liquid Miracle Grow, and 1 mL Kent Essential Elements.  I'm using this as I await my FAF order to arrive.    #37 Fishtal Total Posts : 5467 Scores: 37 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Phytoplankton Culture Thursday, March 22, 2012 9:02 PM (permalink) 0 My Nanno cultures were kept at room temp so it varied a bit. The light was close too so that kept it a little warmer than ambient temp. http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #38 EasterEggs Total Posts : 1946 Scores: 19 Reward points : 735 Joined: 9/22/2011 Location: Saskatoon, SK, CA Status: offline MBI: 590 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Phytoplankton Culture Sunday, March 25, 2012 7:58 AM (permalink) 0 What is room temp in your fish room Tal?  I try to keep mine near 80F so my heaters don't have to work as hard.   #39 Fishtal Total Posts : 5467 Scores: 37 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Phytoplankton Culture Sunday, March 25, 2012 12:18 PM (permalink) 0 It varies by season. 70's and up. http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #40 Online Bookmarks Sharing: Facebook Digg Google del.icio.us StumbleUpon Change Page: < 1234 > | Showing page 2 of 4, messages 21 to 40 of 67 - powered by ASPPlayground.NET Forum Trial Version

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