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•  Rotifer Culture

  Change Page: < 123 > | Showing page 2 of 3, messages 21 to 40 of 47 - powered by ASPPlayground.NET Forum Trial Version Author Message Caesra Total Posts : 317 Scores: 2 Reward points : 195 Joined: 7/4/2011 Location: Cherry Valley, IL, US Status: offline MBI: 80 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Rotifer Culture Monday, July 11, 2011 9:12 PM (permalink) 0 Isn't the idea of keeping the bubble count low, to keep the ph low so that the ammonia is not so toxic?  Can you expand on your belief regarding how much air should but injected?   Thanks   #21 GreshamH Total Posts : 757 Scores: 16 Reward points : 600 Joined: 4/27/2011 Location: SF Bay Area, CA Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Rotifer Culture Monday, July 11, 2011 11:15 PM (permalink) 0 Not how I've heard it play out over the years, it was a long time myth that too much movement could knock eggs of rotifers.   BTW, Gresham Hendee from Reed Mariculture Inc here   I'll be commenting on how we do things.   For our massive rotifer systems we: aerate the heck out of them (read: BOIL) inject O2 continually feed RG Continually dose Cloram-x in a 1:1 ratio with RG (ask us for the formula) daily 50% harvest.. at times 70% harvest system runs ~5K per ml but can be pushed to ~10K per ml when needed.   If you are using paste for rotifers, Cloram-X is a must.  That eliminates ammonia thus giving you no reason to suppress the PH.   #22 Fishtal Total Posts : 5467 Scores: 37 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Rotifer Culture Monday, July 11, 2011 11:18 PM (permalink) 0 Thanks for sharing Gresh! I started this thread a while ago when I was new to things. I need to update it. http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #23 GreshamH Total Posts : 757 Scores: 16 Reward points : 600 Joined: 4/27/2011 Location: SF Bay Area, CA Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Rotifer Culture Monday, July 11, 2011 11:22 PM (permalink) 0   Oh no, was I necroposting ? ? ?  sorry   #24 Fishtal Total Posts : 5467 Scores: 37 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Rotifer Culture Monday, July 11, 2011 11:33 PM (permalink) 0 Originally Posted by GreshamH   Oh no, was I necroposting ? ? ?  sorry Not at all. You just reminded me that I needed to make an update to the original post, which I have done. http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #25 Caesra Total Posts : 317 Scores: 2 Reward points : 195 Joined: 7/4/2011 Location: Cherry Valley, IL, US Status: offline MBI: 80 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Rotifer Culture Monday, July 11, 2011 11:39 PM (permalink) 0 OK, so the suggestion is to use alot of cloram-x..equal doses of paste and coralm-x (I am asking =) )   The idea is the use something like cloram-x to control the ammonia and airiate the heck out of the water to allow densities to rise.  correct?   Now if you wanted to keep a passive culture in place (i.e. maintenance culture(s)) that you do not need currently and are not trying to acheive a high density, but high survival and low maintenance...suggestions?   #26 Umm_fish? Total Posts : 2835 Scores: 10 Reward points : 953 Joined: 11/4/2009 Location: Boulder, CO, US Status: offline MBI: 775 Pnts MBI Site: Marine Aquarium Society of Colorado Re:Rotifer Culture Tuesday, July 12, 2011 10:18 AM (permalink) 0 You have to mix the CloramX (it's a powder). The dosage that Gresham gave me a long time ago (and I can no longer find the original) is 16 teaspoons of CloramX per 1 liter of mix-up water. Then you dose the mix-up water at an equal ratio to algae paste. I just use a 1 liter water bottle with an eyedropper (and in fact, I tend to dose CloramX at a 2:1 ratio).   Good to know about the aeration. I'll turn mine up today. Thanks, Gresh!   I do the same things with my maintenance cultures. I just lower the total water volume of the culture. --Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886   #27 GreshamH Total Posts : 757 Scores: 16 Reward points : 600 Joined: 4/27/2011 Location: SF Bay Area, CA Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Rotifer Culture Tuesday, July 12, 2011 4:04 PM (permalink) 0 IMO regardless of your intentions, the method should remain the same as far as culturing rotifers.... the only thing I would change is the feed rate.  Your culture will only get as dense as the feed source allows.  Cloram-X should be used at all times.   Andy we've changed the ratio I am pretty sure. The RG series needed it to be switched.  I will get the ratio later today, right now I am dealing with getting 3oz Otohime packets stocked up for retail release shortly   FWIW if you really need info from me, email is best:  greshamATreedmariculture.com I try to come check here often but the next few months will be hectic so i doubt I will make it here to respond.    #28 Umm_fish? Total Posts : 2835 Scores: 10 Reward points : 953 Joined: 11/4/2009 Location: Boulder, CO, US Status: offline MBI: 775 Pnts MBI Site: Marine Aquarium Society of Colorado Re:Rotifer Culture Tuesday, July 12, 2011 4:49 PM (permalink) 0 Good luck with marketing the top-dressed oto. Every fish I have show interest, even when they are way too big to take the feed. Good stuff.   Thanks! --Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886   #29 GreshamH Total Posts : 757 Scores: 16 Reward points : 600 Joined: 4/27/2011 Location: SF Bay Area, CA Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Rotifer Culture Tuesday, July 12, 2011 5:18 PM (permalink) 0 That's next.  I'm working on Otohime at the moment.  TDO won't be released till the entire line is released and that date is TBA   FWIW we're doing A,B1,B2,C1,C2,EP1 and EP2 in TDO.  EP1 is 1.7mm and EP2 is 2.3mm so there will be large sizes for large broodstock.    #30 THEJRC Total Posts : 1006 Scores: 4 Reward points : 555 Joined: 10/23/2009 Location: Colorado Springs, CO, US Status: offline MBI: 210 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Rotifer Culture Tuesday, July 12, 2011 7:27 PM (permalink) 0 Nice to hear on the EP1 and EP2 Gresh! Pelagically yours, ~J         #31 Caesra Total Posts : 317 Scores: 2 Reward points : 195 Joined: 7/4/2011 Location: Cherry Valley, IL, US Status: offline MBI: 80 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Rotifer Culture Tuesday, July 12, 2011 9:00 PM (permalink) 0 OK, so wanting to get a good idea of paste to cloram-x...just ordered some.  Don't think it shipped today, so I wont see it until next week...so ultimate will ahve to due, although the last two cultures are going down hill.  I added some extra Ulitmate to the bucket I turned the air up and they look like they are all goners..so down to one now.   Will try it the way you suggest gresh once I have the cloram-x, assuming I have any rotifers left.  just one culture still running and it is way to short on count to try to split, although I suppose I could try a very small container and see if I can get some density in there to build.   In anycase, the question is...so we are trying to match the actual paste to the cloram-x mixture?  So just a hypothetical..if I put 16 tsp of paste in a 1L for dilution and a cloram-x mix of 16 tsp to 1L, then I would dose both in a 1:1. correct?   #32 GreshamH Total Posts : 757 Scores: 16 Reward points : 600 Joined: 4/27/2011 Location: SF Bay Area, CA Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Rotifer Culture Thursday, July 14, 2011 11:18 AM (permalink) 0 email is bet is you have a time senstive question.  I just happen to be on hold so I hit the forum   no not correct.  You do not dilute the IA "paste".  1 drop of paste (its liquid, not paste) to 1 drop of the Cloram-X mixture.  Like I said earlier, I am pretty sure the formula has changed so 16 tsp may not be the number now.   #33 Caesra Total Posts : 317 Scores: 2 Reward points : 195 Joined: 7/4/2011 Location: Cherry Valley, IL, US Status: offline MBI: 80 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Rotifer Culture Saturday, July 16, 2011 10:08 PM (permalink) 0 Well, nicely so my cloram-x showed up today (saturday)..odd but happy.  Now the question is what ration should I use?  I will use the 16 tsp for now.   #34 Umm_fish? Total Posts : 2835 Scores: 10 Reward points : 953 Joined: 11/4/2009 Location: Boulder, CO, US Status: offline MBI: 775 Pnts MBI Site: Marine Aquarium Society of Colorado Re:Rotifer Culture Sunday, July 17, 2011 3:42 PM (permalink) 0 Good luck, Caesra! I hope it works better for you. --Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886   #35 Caesra Total Posts : 317 Scores: 2 Reward points : 195 Joined: 7/4/2011 Location: Cherry Valley, IL, US Status: offline MBI: 80 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Rotifer Culture Wednesday, July 20, 2011 10:15 PM (permalink) 0 So far I have two running that seem to be doing ok.  following all of your guy's advice so far and things seem to be improving.  Still running one at low bps and one at high...the lower one seems to be doing better in reproduction, but they are both going good.   #36 Lance6270 Total Posts : 32 Scores: 0 Reward points : 90 Joined: 9/25/2011 Location: Wylie, TX, US Status: offline MBI: 180 Pnts MBI Site: Dallas/Ft Worth Marine Aquarium Society Re:Rotifer Culture Monday, September 26, 2011 6:15 PM (permalink) 0 OK I thought I had posted this but I cannot seem to find the info on this. I see it is a 2:1 ratio of the Paste to ClorAm-x but that is the powder that you mix. What if you are using the bottle of the Liquid as that is what I am using. Not sure if it would be the same but it seems as it would as it is a 16oz bottle and that seems to be what everyone is mixing up. I an dosing 15 drops of ClorAm-x to 30 drops of  Rotigrow. If this is not right can someone post what I should be mixing as I do this twice a day to feed the rotifers and that is per 5g bucket.   #37 Fishtal Total Posts : 5467 Scores: 37 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Rotifer Culture Monday, September 26, 2011 6:44 PM (permalink) 0 I haven't used the pre-mixed stuff so I'm not sure. http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #38 Macae fernandes Total Posts : 72 Scores: 0 Reward points : 128 Joined: 5/2/2011 Location: Funchal , Madeira, PT Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Rotifer Culture Friday, October 5, 2012 6:44 PM (permalink) 0 Hi all, Sorry to be susing this thread to make some questions but i realy need some advice, cause someone said, " the more i read the more i missunderstand some rules of culturing this guys ". I want to try and breed clownfisehs, my pair is hatching for some months now, and i have already all the stuff i think i need for them: - Ive already bubbling and geting greener a 7x 2Liter Bottles with Nannochloropsis, and im waiting to get a sample of rotifer type L.   - Im thinking on culturing in a 5G Bucket with 4G of culture water at 1.020, with a slow bubbling rigid tube ( 3-6 bps?).   I think i should start using the double of amount of the water in wich the rotifer arrives here, and then doubling each day the amount till i get 4 G, is this right?   My serious questions are: Do i need or is advisable to get rotigro+ and cloramx- or RG complete, to add to the phyto or this is enough for feeding the rot´s?   How much phyto shall i give daily? the same amount of the water i take every day? Shall i toilet the rot´s i sieve every day when replacing 25% of the water or i shall put them back in culture bucket?   If i understood correctly you guys say i shouldnt clean the muck on the bottom of the culture? Some say, just clean all the bucket once a week and put new SW...   Plz guys, Be patient and just try to answer all this questions and if possible give me some advices on how to be sucessfull on culturing this rot´s.   Ty   #39 Fishtal Total Posts : 5467 Scores: 37 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Rotifer Culture Friday, October 5, 2012 8:30 PM (permalink) 0 Originally Posted by Macae fernandes Hi all, Sorry to be susing this thread to make some questions but i realy need some advice, cause someone said, " the more i read the more i missunderstand some rules of culturing this guys ". I want to try and breed clownfisehs, my pair is hatching for some months now, and i have already all the stuff i think i need for them: - Ive already bubbling and geting greener a 7x 2Liter Bottles with Nannochloropsis, and im waiting to get a sample of rotifer type L. - Im thinking on culturing in a 5G Bucket with 4G of culture water at 1.020, with a slow bubbling rigid tube ( 3-6 bps?). I think i should start using the double of amount of the water in wich the rotifer arrives here, and then doubling each day the amount till i get 4 G, is this right? My serious questions are: Do i need or is advisable to get rotigro+ and cloramx- or RG complete, to add to the phyto or this is enough for feeding the rot´s? How much phyto shall i give daily? the same amount of the water i take every day? Shall i toilet the rot´s i sieve every day when replacing 25% of the water or i shall put them back in culture bucket? If i understood correctly you guys say i shouldnt clean the muck on the bottom of the culture? Some say, just clean all the bucket once a week and put new SW... Plz guys, Be patient and just try to answer all this questions and if possible give me some advices on how to be sucessfull on culturing this rot´s. Ty If you're feeding live phyto you don't need to feed RGrow too. I prefer using RGrow instead of live because it's easier and cheaper over the long run.   How much phyto you need to feed will depend on which product you use and how dense your culture is. Ideally, you want to feed enough so the water doesn't go clear between feedings. Drip feeding is the best way to feed but I just feed twice per day, 12 hours apart, and have no problems.   I only harvest rotifers when I need them or when someone else wants some. I know this is not the best thing to do but it works for me. About once a month I will "refresh" my cultures by siphoning out 1/2 of the culture and add it to a new bucket 1/2 full with fresh SW.  http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #40 Online Bookmarks Sharing: Facebook Digg Google del.icio.us StumbleUpon Change Page: < 123 > | Showing page 2 of 3, messages 21 to 40 of 47 - powered by ASPPlayground.NET Forum Trial Version

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