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Breeding Journal, Species: Pseudocheilinus hexataenia (sixline wrasse)
Saturday, January 16, 2010 12:55 AM
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Breeding Journal DataSheet This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Pseudocheilinus hexataenia Social Structure: Haremic (Large male with several females, I keep as a pair) Size of Individuals: M-3", F-2" Age of Individuals: Unknown, my care for 8-9 months Date added to Tank: March or so Broodstock Tank Details Size of Tank: 55 gal. drum Substrate Details: A little sand Filtration Details: Connected to 1,400 gal. system with skimmers, algae tank, DSB tank Water Changes: ~100 gals. every 2 weeks Water Temperature: 78-82 Lighting: Barely, just a FW hood over the 3.5' deep tank. Lighting Cycle: 14 hours Other Tank Inhabitants: They used to have a couple chromis and a peppermint shrimp. Now none (shrimp lives in overflow) Broodstock Feeding Details Food Types: Seafood mash, + everything else I can find Feeding Schedule: 4-5x/day Spawning Details Date of First Spawn: 5-24-09 Spawn Time of Day: 8pm Dates of Consecutive Spawns: pretty much every day CourtShip Details: Here's a video http://www.youtube.com/watch?v=Ry9pB600cEE&feature=player_embedded Egg Size: ~750 microns, have gotten a little bigger with broodstock maturation Egg Color: clear Egg Count: Varies from ~50-150 Hatch Details Hatch Date: June 1, 2009 or so Hatch Time of Day: ~24 hours after spawn # Days after Spawn: ~1 Larve Description: Well, they aren't larvae yet. They are prolarvae. ~2mm long, clear, no eyes, no working mouth, large yolk sac ProLarval Tank Details Temperature: Room Size of Larval Tank: A petri dish, I've also tried yogurt containers (large) and now a quart canning jar Substrate Details: Glass Other Tank Decor: None Filtration Details: None Lighting: Room Lighting Cycle: Room Water Changes: I've started doing tiny, tiny WCs with a pipette. Larval Feeding Details Food Types: I'm trying to slowly introduce a flagellate to their culture to see if they will take it. Feeding Schedule: Metamorphosis/Settlement Date of Settlement Start: Days after Hatch: Date of Settlement End: Description of Fry: Grow-Out Tank Details Temperature: Size of Grow-Out Tank: Substrate Details: Other Tank Decor: Filtration Details: Lighting: Lighting Cycle: Water Changes: Size at Transfer: Age at Transfer Grow-Out Feeding Details Food Types: Feeding Schedule: Additional Information Miscellaneous Information: You will be required to provide photographic evidence in this thread of each event submitted for the MBI Program. If your thread does not contain these photos the MBI Committee will not be able to approve your reports.
<message edited by Umm_fish? on Tuesday, February 2, 2010 10:51 PM>
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Re:Breeding Journal, Species: Pseudocheilinus hexataenia (sixline wrasse)
Saturday, January 16, 2010 12:56 AM
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This is another one that I will be filling in slowly. My efforts with these are ongoing. I'm slowly teaching myself the things I need to learn to have a chance with them.
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Re:Breeding Journal, Species: Pseudocheilinus hexataenia (sixline wrasse)
Saturday, January 16, 2010 1:00 AM
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Wrasses are pelagic spawners, so the broodstock tank is designed to make it easy to collect eggs. Broodstock tank: Water is pumped into the bottom of the barrel and slowly makes its way up to the overflow: The green bucket is an external overflow. Inside there's enough room for an egg collector. Egg collector:
<message edited by Umm_fish? on Saturday, January 16, 2010 1:13 AM>
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Re:Breeding Journal, Species: Pseudocheilinus hexataenia (sixline wrasse)
Saturday, January 16, 2010 1:06 AM
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Egg at 40x (I think): Egg at 100x: Embryo from a later batch of eggs, 15 hours post-collection: And a prolarva (they are around 2mm):
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Re:Breeding Journal, Species: Pseudocheilinus hexataenia (sixline wrasse)
Saturday, January 16, 2010 1:08 AM
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Re:Breeding Journal, Species: Pseudocheilinus hexataenia (sixline wrasse)
Saturday, January 16, 2010 1:15 AM
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Another prolarva, attached to a huge yolk sac with oil droplet:
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Re:Breeding Journal, Species: Pseudocheilinus hexataenia (sixline wrasse)
Saturday, January 16, 2010 1:16 AM
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Composite prolarva, with backlighting: And without:
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Re:Breeding Journal, Species: Pseudocheilinus hexataenia (sixline wrasse)
Saturday, January 16, 2010 1:18 AM
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Re:Breeding Journal, Species: Pseudocheilinus hexataenia (sixline wrasse)
Saturday, January 16, 2010 1:23 AM
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And I love this photo. I pulled out the camera and handheld this photo, eggs floating in a yogurt container, flash below eggs and diffused through side of container.
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Re:Breeding Journal, Species: Pseudocheilinus hexataenia (sixline wrasse)
Saturday, January 16, 2010 1:24 AM
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And, what the plankton predators see (or not) when trying to eat one of the little guys:
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Re:Breeding Journal, Species: Pseudocheilinus hexataenia (sixline wrasse)
Saturday, January 16, 2010 1:27 AM
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And this last one catches us up:
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Re:Breeding Journal, Species: Pseudocheilinus hexataenia (sixline wrasse)
Saturday, January 16, 2010 1:30 AM
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Goal for soon: I have a flagellate that contaminates my rots to very high numbers (1000s per mL). I want to do another egg collection and see whether the flagellate might make a first food. Ya never know....
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Re:Breeding Journal, Species: Pseudocheilinus hexataenia (sixline wrasse)
Sunday, January 31, 2010 10:47 PM
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Checked the sixlines for for spawning again tonight: 9:18 pm. Here's one of the eggs, 30 minutes old.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Breeding Journal, Species: Pseudocheilinus hexataenia (sixline wrasse)
Monday, February 1, 2010 8:13 PM
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Differences in developmental rates in sixline wrasse embryos. All of these are 19.5 hours old. All photographs are taken in situ in a one quart canning jar.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Breeding Journal, Species: Pseudocheilinus hexataenia (sixline wrasse)
Tuesday, February 2, 2010 10:52 PM
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Differences in developmental rates are still evident in the sixline wrasse prolarvae. All of these are now 43.5 hours post-spawn. The fish have tolerated very minor water changes (just with a pipette, so slllooooowwww) and the addition of small amounts of the flagellate+greenwater that I want to try as first food. Oil globule and yolk are to the left of this larva. Oil globule and yolk are below the larva in this shot. Focus is a little lower in this shot so you can see fin development. A question: We're now at about 49 hours post-spawn and some of the prolarvae have adopted a tail-high stance in the water column before swimming off. None of them are doing it all the time. What does it mean? Has the oil globule been absorbed?
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Breeding Journal, Species: Pseudocheilinus hexataenia (sixline wrasse)
Thursday, February 4, 2010 11:12 AM
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At least one of them is still holding on for me close to 86 hours post-spawn, which is a new record for me. I did a small water change and added more possible prey. Unfortunately I wasn't able to get any photos.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Breeding Journal, Species: Pseudocheilinus hexataenia (sixline wrasse)
Thursday, February 4, 2010 9:55 PM
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I mis-added the hours earlier. My little guy is up over 120 hours now! And I think I saw it strike at _something._ Still no photos, sorry. But we've blown by my past success!
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Breeding Journal, Species: Pseudocheilinus hexataenia (sixline wrasse)
Thursday, February 4, 2010 10:59 PM
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That's Awesome!
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Re:Breeding Journal, Species: Pseudocheilinus hexataenia (sixline wrasse)
Friday, February 5, 2010 9:36 AM
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Looks like I finally lost him, but now I have a new benchmark. Water changes seem like a really good idea, for one.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Breeding Journal, Species: Pseudocheilinus hexataenia (sixline wrasse)
Friday, April 2, 2010 12:05 AM
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Well, it's been a while, but I made another collection of sixline eggs tonight (138). Look like the one on the left here has made the first cleavage.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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