Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Tigriopus japonicus
Species description: Intertidal Harpacticoid Copepod similar to Tigriopus californicus but posessing smaller size approx 100-800 micron
Culture source (link if possible): unknown, isolated from cross cultures
If algae, CCMP # (ref http://ccmp.bigelow.edu/ ): Culturing Vessel Details Salinity: 1.022
Temperature: ambient 72*F
Vessel description: 4 quart sterilite snapware container (tall vertical)
Lighting description: 3 dual tube normal output flourescents using 34 watt 3500K bulbs
Lighting cycle: 14 hours on, 10 hours off
Aeration description: rigid airline placed near bottom of vessel, medium bubble rate (approximately 20 bubbles per second)
Methodologies Split methodology: Cultures are split based on water "clearing" rate. cultures are fed a mixture of live algae cultures (typically nannochloropsis, tetraselmis, thallasiosira, and gymnodinium) replacing one third of the culture medium, a second third is replaced with ASW at 1.022 salinity. the remaining third is retained.
Culture medium description: 1.022 ASW and a variety of algae from cultures
Cell count: (if known) N/A typical population count averages 4-12 specimens per ml
Reference links: Additional Information Notes: Fairly common in cultures, Tigriopus is a staple harpacticoid within the marine hobby. These specimens were isolated from my display several years ago and have performed well in monoculture. Culture density can be raised by placing sections of egg crate vertically in the culture vessels to provide for more surface area. Are extremely resiliant to temperature and salinity swings and the smaller size aids in feeding response from smaller live feeders such as pipefish. Due to smaller size and fast regeneration time this may be a species worth studying for a first or second food source.
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<message edited by cmpenney on Tuesday, May 4, 2010 9:23 AM>