Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Tigriopus californicus
Species description: Intertidal Harpacticoid Copepod Tigriopus californicus is one of the most common in the hobby with average size between 200 and 1500 micron
Culture source (link if possible
): www.reefnutrition.com marketed as "Tigger pods"
If algae, CCMP # (ref
http://ccmp.bigelow.edu/ ): Culturing Vessel Details Salinity: 1.022
Temperature: Ambient 78*F
Vessel description: 4 quart Sterilite snapware containers
Lighting description: 3 dual tube normal output flourescent fixtures mounted vertically using 3500K bulbs
Lighting cycle: 14 on 10 off
Aeration description: rigid airline with no airstone suspended just off the bottom of the vessel, aeration rate of approx 20 bubbles per second
Methodologies Split methodology: Split based on water clearing rate, harvest is performed at split. two thirds of culture is harvested with one third being replaced with various algae cultures and one third replaced with ASW mixed to 1.022
Culture medium description: ASW and algae direct from other cultures, typical algae mixture consists of Nannochloropsis, Tetraselmis, Thallasiosira, and Gymnodinium
Cell count: (if known) varies, on average counts are 2-4 specimens per ml sampled
Reference links: http://www.copepodgeek.com/Copepod%20culture%20handbook/Copepod%20Culturing.html http://www.luciopesce.net/copepods/ http://invertebrates.si.edu/copepod/ http://oceanpods.com/ Additional Information Notes: possibly the most common species in the hobby, while a staple with feeding for adult fish it is assumed that even the nauplii of this particular species is too large and lacks the proper activity (rests on surfaces and does not spend much time in water column) to be effectively used for early rearing and first food stages. A good starter species for anyone looking to learn food culturing. While argument is abound regarding whether or not the species is temperate or tropical in nature populations continue to be robust and very tolerant of changing conditions.
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<message edited by cmpenney on Tuesday, May 4, 2010 9:24 AM>