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Culture Journal, Species: Oxyrrhis marina
Thursday, September 30, 2010 2:10 AM
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Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Oxyrrhis marina Species description: Heterotrophic dinoflagellate, ~ 15 microns wide x ~ 30 microns long Culture source (link if possible ): Volunteer contaminate in my T-Iso culture If algae, CCMP # (ref http://ccmp.bigelow.edu/ ): CCMP 604, 605, 1739, 1788, 1795 Culturing Vessel Details Salinity: 1.010 - 1.015 Temperature: Ambient room temp; ~ 72 - 74 F Vessel description: 500 ml Erlenmeyer flasks, 1000 ml Erlenmeyer flasks, 2 L media jars Lighting description: Ambient room light Lighting cycle: N/A - this species does not need light to grow. Aeration description: Varies; they do best with moderate aeration Methodologies Split methodology: Harvest 1/4 to 1/2 of the volume of each container daily. Split or feed to copepods as desired. Culture medium description: 8 drops / liter twice daily of varied preserved algal pastes. Typical feeding for 1 liter would be 4 drops of Roti-Grow Plus (primarily Nannochloropsis) plus 4 drops of N-Rich (Primarily T-Iso, Pavlova, and other EPA-rich algae) twice daily. Also add 1 drop of ClorAm-X solution (12 tsp. of ClorAm-X powder dissolved in 1 liter of water) per drop of food added at each feeding. Cell count: Very rough estimate is 50,000 - 100,000 cells / ml. Reference links: http://sabella.mba.ac.uk/...ng_Oxyrrhis_marina.pdf http://www.int-res.com/ar.cles/meps/2/m002p229.pdf http://www.springerlink.c...tent/f204x254318278j0/ http://cat.inist.fr/?aMod...eN&cpsidt=16712370 http://www.int-res.com/ar.cles/ame/10/a010p307.pdf Additional Information Notes: I first discovered this contaminate in my A. panamensis cultures in February, 2010. I ultimately discovered that the O. marina had contaminated my T-Iso cultures. I've been culturing the O. marina ever since.  You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by JimWelsh on Sunday, October 3, 2010 10:14 PM>
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Re:Culture Journal, Species: Oxyrrhis marina
Tuesday, October 5, 2010 8:31 AM
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Thanks for the information Jim  What magnification is that?
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Re:Culture Journal, Species: Oxyrrhis marina
Wednesday, October 6, 2010 3:37 PM
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Can you snap some pics of the cultures in the Erlenmeyer flasks?
Thanks
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Re:Culture Journal, Species: Oxyrrhis marina
Wednesday, October 6, 2010 4:49 PM
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The images were taken at a magnification of 100x, Doni Marie.
@Zooid, pictures to follow. Gonna be boring, though!
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Re:Culture Journal, Species: Oxyrrhis marina
Wednesday, October 6, 2010 5:03 PM
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LOL...that's ok....I just wanted to see the color.
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Re:Culture Journal, Species: Oxyrrhis marina
Wednesday, October 6, 2010 5:05 PM
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When just fed, kinda yellowish. When hungry, kinda milky whitish. I'll post pics of both later.
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Re:Culture Journal, Species: Oxyrrhis marina
Wednesday, October 6, 2010 7:25 PM
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Very nice pictures. Is that with phase contrast?
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Re:Culture Journal, Species: Oxyrrhis marina
Wednesday, October 6, 2010 7:29 PM
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Some phase contrast, one using Nomarski lighting (whatever that is). The microbiology folk at the lab I work at helped me with these.
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Re:Culture Journal, Species: Oxyrrhis marina
Thursday, October 7, 2010 5:12 AM
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 Originally Posted by JimWelsh
The images were taken at a magnification of 100x, Doni Marie.
Thanks Jim. I got my microscope this week and I'll need your assistance with confirming my cultures (both O. marina and the panamensis)  I THINK they are both doing really well. I am using 1.025 salt water for both with no apparent issues. I did have to strain my O. marina with a 52 um a few times due to rotifers. 
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Re:Culture Journal, Species: Oxyrrhis marina
Thursday, October 7, 2010 7:26 AM
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Doni: Any time I do anything with those cultures, I strain them through a rot screen. I've not had a lot of rot issues, though. The rots won't eat the O. marina and the O. marina hugely out-compete the rots when you get the food concentrations right. The bigger problem I've had has been with a large ciliate and with bacteria. But both of those also seem to be controllable with culture container changes and food supply control. Jim: Yes, very nice photos! I need one of those scopes.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Culture Journal, Species: Oxyrrhis marina
Thursday, October 7, 2010 8:57 AM
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Andy, with a hand held 10x magnifier, I can see a very dense quantity of uniform specks. 'Specks' is all I can say without looking at them under the microscope but they are much smaller than rots.
I am not familiar with ciliates and what they look like.
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Re:Culture Journal, Species: Oxyrrhis marina
Thursday, October 7, 2010 9:06 AM
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Sounds like them, though we won't know for sure without the scope. I can see the clouds resolve into little individual specks without magnification if I use backlighting. That's how I do my daily culture checks, in fact. The ciliates I had problems with are much larger than O. marina, more on the order of slightly smaller than a rot. But get those slides in. We'll know for sure really quickly.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Culture Journal, Species: Oxyrrhis marina
Tuesday, October 19, 2010 9:14 PM
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Originally Posted by Zooid
Can you snap some pics of the cultures in the Erlenmeyer flasks?
Thanks
Ok, I know the pictures are late, but here they are: Before feeding:  After feeding: 
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Re:Culture Journal, Species: Oxyrrhis marina
Wednesday, October 20, 2010 4:20 PM
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Thanks, Jim. I always wanted to see how you are culturing them.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Culture Journal, Species: Oxyrrhis marina
Friday, October 29, 2010 5:30 AM
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I wish I had the money for a few gallon sized Erlenmeyers hehe. Thanks for the pics. Looks similar to mine. Andy's always seems denser than mine though
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Re:Culture Journal, Species: Oxyrrhis marina
Friday, October 29, 2010 11:41 AM
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Here's a thought, Gale: What kind of lighting are yours under? My cultures that get the least lighting are consistently the best performers. Maybe the light encourages algae and bacterial growth?
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Culture Journal, Species: Oxyrrhis marina
Saturday, August 13, 2011 4:40 PM
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I'm going to resurrect this journal. I first noticed this organism as a contaminate in my copepod cultures in February, 2010. I confirmed the tentative ID in March, 2010. I've not been culturing these deliberately recently, so I'm just now re-"establishing" this culture for the purposes of this journal, and the MBI Culture Continuation report I'll be filing soon (after the 90 days have passed). I know that I could probably file a report based on the success with this species that I've already documented, but want to demonstrate how easy it is to establish and maintain cultures of these little guys. My intention is to act as an inspiration to others who may wish to culture them as live feeds for copepods and/or other creatures.
To "Establish" this culture, I took the water from a crashed Moina salina culture and examined a sample under the microscope. I could see numerous O. marina, and also some Euplotes. I filtered the crashed Moina culture water through a coffee filter into a clean flask. I examined with the scope again, and there were still too many Euplotes for my liking, so I filtered again, this time through three coffee filters stacked. After that, I could see no Euplotes in any of several samples I checked with the scope, but still had numerous O. marina. I'm initially feeding them lightly with live Isochrysis, but will switch to using preserved algae pastes soon.
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Re:Culture Journal, Species: Oxyrrhis marina
Saturday, August 13, 2011 10:03 PM
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keep the info comming =)
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Re:Culture Journal, Species: Oxyrrhis marina
Sunday, August 14, 2011 9:20 AM
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Good luck, Jim! 
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Culture Journal, Species: Oxyrrhis marina
Sunday, March 15, 2015 2:50 AM
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I hate to resurrect old threads but I'm having trouble finding much info on these guys. Culturing seems simple enough, but aside from A panamensis, what other copepods is this ciliate useful for? I'm curious if its been tested as a food source for species with more potential such as P. crassirostris.
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