Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Isochrysis galbana
Species description: Motile phytoflagellate 4-6 microns
Culture source (link if possible
): SeahorseSource.com
If algae, CCMP # (ref
http://ccmp.bigelow.edu/ ): CCMP 462, 1323, 1611
Culturing Vessel Details Salinity: 1.019
Temperature: Ambient room temp; 72 - 74 F NOTE: The culture itself actually gets up to abou 78 - 80 F.
Vessel description: 2 liter borosilicate media culture jars
Lighting description: 1 warm white 40W fluorescent + 1 daylight 40W fluorescent. NOTE: That was the old phyto station. The new one is using 2 x 6500K 21 Watt T5 bulbs in each fixture (one fixture per shelf).
Lighting cycle: 18h on / 6h off
Aeration description: vigorous
Methodologies Split methodology: Split 4:1 (500 ml of dense culture + 1500 ml of fresh sterilized water)
Culture medium description: 1200 ml of recycled tank water + 300 ml of fresh RO water microwaved to 85 C (185 F), plus 0.75 ml of FAF Micro Algae Grow (sufficient to make a Guillard F/2 solution for 2000 ml total volume).
Cell count: At its densest, the cell count is roughly 15,000,000 - 20,000,000 cells/ml, based on Secchi readings and charts.
Reference links: Additional Information Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by JimWelsh on Tuesday, March 27, 2012 10:05 AM>