Culture Journal, Species: Brachionus plicatilis

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Hellaenergy
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Culture Journal, Species: Brachionus plicatilis - Thursday, September 30, 2010 11:50 AM
Culturing Journal DataSheet



General
Species:  Brachionus plicatilis
Species description:  "L" type marine species
Culture source (link if possible):  http://www.reedmariculture.com/rotifer/instant.asp

Culturing Vessel Details
Salinity:  1.025
Temperature:  Room Temp ~70F
Vessel description:  5g White Bucket
Lighting description: Ambient Room Lighting 
Lighting cycle:  None
Aeration description:  Small Air Pump -> Valve (set low) -> Suction Cup + Tube on the side of the vessel.

Methodologies
Split methodology:  I harvest 1/4 to 1/3 the culture daily. The water volume is replaced with fresh saltwater and more RotiGrow Plus.

Culture medium description: 

RotiGrow Plus is added until water is a light green color. In the bucket a clear plastic cup is used to harvest and observe the coloration.

Cell count:
Not known

Reference links:   http://www.reedmariculture.com/rotifer/grow.asp

Additional Information

 

Notes: 








<message edited by Hellaenergy on Wednesday, December 1, 2010 2:43 PM>
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Hellaenergy
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Re:Culture Journal, Species: Brachionus plicatilis - Wednesday, December 1, 2010 2:18 PM
I've moved to a 5g white bucket exclusively. I feed 19ml of RotiGrow Plus a day. I've also removed the lighting. Here are some rotifer pics under the microscope:
 

 

 

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Umm_fish?
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Re:Culture Journal, Species: Brachionus plicatilis - Thursday, December 2, 2010 9:10 AM
That's a _lot_ of food, but I run them at full-strength saltwater and probably don't have as high a population as you do.
 
Nice photos!
--Andy, the bucket man.
"Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886