Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Brachionus rotundiformis (SS)
Species description: 80-120 um
Culture source (link if possible
): (L strain:
https://3kserver7.com/~frank/secure/agora.cgi?cart_id=3951867.19705*Ry60q8&product=LIVE_CULTURES 
(L strain:
http://www.reed-store.com/shop.cfm/Rotifer-Products/Rotifers-Live-and-Concentrate/ (S strain:
http://www.seahorsesource.com/cgi-bin/shop/search.cgi?&category=Foods-Live If algae, CCMP # (ref
http://ccmp.bigelow.edu/ ): Culturing Vessel Details Salinity: Was 1.019 but I increased it to 1.025-6
Temperature: room temp (@72-77f)
Vessel description: Bucket, tank or plastic planter
Lighting description: ambient lighting
Lighting cycle: 14 hrs on/10 hrs off
Aeration description: gentle airstone
Methodologies Split methodology: I try to remove some daily but usually it is only twice per week (@ 25% harvested each time)
Culture medium description: Algae paste twice daily enough to lightly tint the water green.
Cell count: I've never counted but it stays VERY dense
Reference links: Additional Information Notes: After a few initial culture crashes, I've managed to keep a continuous culture going since 2008. I combined B. plicatis and B. rotundiformis at one point but the smaller rotifers have dominated.
**The culture ranges 80-120 um (ss) per an accurate sizing from an aquatic biologist on November 2010**
I had someone size them so I would know for sure!
Photos to come~
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If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by woodstock on Thursday, November 18, 2010 10:20 AM>