Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Brachionus plicatilis
Species description: "L" Type Marine
Culture source (link if possible
): http://www.reedmariculture.com/rotifer/instant.asp If algae, CCMP # (ref http://ccmp.bigelow.edu/ ): Culturing Vessel Details Salinity: Varies (see additional information below) - Update 1/22/11: Salinity is now kept at 1.015
Temperature: ambient room temperature
Vessel description: 5 gallon carboy and/or 5 gallon bucket - Update 1/22/11 omitted the use of 5 gallon buckets
Lighting description: Room lighting (none specifically set up for the rotifers)
Lighting cycle: No specific cycle
Aeration description: Rigid airline (without airstone) placed in bottom middle of inverted carboy or taped to the side of a 5 gallon bucket set to low flow
Methodologies Split methodology: Daily: Remove amount needed for larvae and reef tanks
Once Weekly: Split 50% - restart in clean carboy and/or 5 gallon bucket - Update 1/22/11: 100% water changes once weekly
Twice Weekly: Remove 50% (feed the reef tanks with the extra) and top off with new saltwater
Culture medium description: RotiGrow Plus fed twice daily (morning & night) to keep a lime green color at all times
Cell count: (if known)
Reference links: Additional Information Notes:
Culture started on 7/4/10:
I keep the salinity in each vessel differently. The reason for this, once my larvae hatch, I slowly decrease their salinity therefor, I want the rotifer salinity to match. As the fry get older, I slowly increase their salinity and so I slowly increase the rotifers salinity. At any given time, my rotifer salinity can range from 1.019 to 1.023. As I understand it, rotifers can handle a .002 change in salinity but I prefer to keep them matched up as close as possible.
Update 1/22/11:
Salinity is kept at 1.015 instead of matching larval tanks & added 100% water change once weekly to maintenance schedule.
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<message edited by cmpenney on Saturday, January 22, 2011 9:56 AM>