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•  Breeding Journal, Species: Liopropoma mowbrayi

  Change Page: < 12345 > | Showing page 3 of 5, messages 41 to 60 of 85 - powered by ASPPlayground.NET Forum Trial Version Reports tied to this Journal Author Message mPedersen Total Posts : 3450 Reward points : 1376 Joined: 2/27/2009 Location: Duluth, MN, US Status: offline MBI: 4975 Pnts MBI Site: Lake Superior Marine Aquarium Club Re:Breeding Journal, Species: Liopropoma mowbrayi Monday, July 18, 2011 7:10 PM (permalink) Formalin or Hydrogen Peroxide baths for egg cleaning...?  Tried any of that?   #41 mPedersen Total Posts : 3450 Reward points : 1376 Joined: 2/27/2009 Location: Duluth, MN, US Status: offline MBI: 4975 Pnts MBI Site: Lake Superior Marine Aquarium Club Re: Breeding Journal, Species: Liopropoma mowbrayi Monday, July 18, 2011 7:16 PM (permalink) Originally Posted by Arc Katana Yeah one second MP, let me upload some stuff of the system Ill be putting some swissguard into on saturday. Youtube clip of v1.0 - (On 1.1 now)   So any updates on 1.1?  I'm particularly curious about the design of how you're separating the eggs from the water that's being drawn off...   #42 Arc Katana Total Posts : 929 Reward points : 1293 Joined: 10/20/2009 Status: offline MBI Site: Marine Breeding Initiative (MBI) Re: Breeding Journal, Species: Liopropoma mowbrayi Monday, July 18, 2011 8:37 PM (permalink) After working with the system, its been easier to just turn the pumps off and collect the eggs after they spawn.  A little more time consuming, but less moving parts!   I actually made a mesh "basket" that sat in the egg collector, to get eggs I'd just pull the basket up and put it in the egg holder.  I've got pics somewhere I'll look for em.   Haven't done anything from a chemical standpoint - I've re-read your experiences with the pygmy angels on the old board, seemed like you got better results without any of that stuff.  I think I'll try a drop of Iodide for the next ones.   #43 Umm_fish? Total Posts : 2835 Reward points : 953 Joined: 11/4/2009 Location: Boulder, CO, US Status: offline MBI: 775 Pnts MBI Site: Marine Aquarium Society of Colorado Re: Breeding Journal, Species: Liopropoma mowbrayi Tuesday, July 19, 2011 8:45 AM (permalink) I got _much_ better hatches and more vigorous sixline prolarvae with hydrogen peroxide baths. I still had trouble getting them to feeding stages. I think they might be sensitive to tiny amounts of ammonia, but that's still theory. I'm still cycling the new tank that I want to use for hatching. --Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886   #44 Arc Katana Total Posts : 929 Reward points : 1293 Joined: 10/20/2009 Status: offline MBI Site: Marine Breeding Initiative (MBI) Re: Breeding Journal, Species: Liopropoma mowbrayi Tuesday, July 19, 2011 10:30 AM (permalink) Andy and Matt, what percentages are you using with the peroxide?  I'll give that a shot, can't hurt right?  I'm not worried about first foods, I've got a kazillion parvolacanus pods and I can always collect zooplankton every day.   #45 Arc Katana Total Posts : 929 Reward points : 1293 Joined: 10/20/2009 Status: offline MBI Site: Marine Breeding Initiative (MBI) Re:Breeding Journal, Species: Liopropoma mowbrayi Tuesday, July 19, 2011 3:40 PM (permalink) I found this on the web - do these ratio's look right? http://www.soest.hawaii.e...ANT/Makai/nov98/2.html   *NOTE LINK IS 404 DEAD* Summary: They found that fish infected with the parasite Amyloodinium Ocellatum on their gills, were successfuly treated by peroxide. At the start of the study, the fish averged 37 parasites per gill sample. After being exposed to 75ppm to 100ppm for 24 hours, the parasites were eradicated. 100ppm is equivalent to only about 2 or 3 drops of 35% peroxide in 8oz of water! The untreated fish still had an average of 28 parasites at the end of the study.   I was thinking of adding 3 drops to 8oz of water that the eggs are in for the first 24 hrs, then collect eggs and move them to a non-treated new ASW mix.  Sound about right?   #46 Duck Total Posts : 466 Reward points : 465 Joined: 12/14/2010 Location: Barrow in Furness, Cumberland Status: offline MBI: 760 Pnts MBI Site: Marinefish-uk.co.uk Re: Breeding Journal, Species: Liopropoma mowbrayi Tuesday, July 19, 2011 3:42 PM (permalink) I use 1ml per ltr H2O2 @12% solution. The solution % you can buy vary so always chech that first.   #47 Arc Katana Total Posts : 929 Reward points : 1293 Joined: 10/20/2009 Status: offline MBI Site: Marine Breeding Initiative (MBI) Re:Breeding Journal, Species: Liopropoma mowbrayi Tuesday, July 19, 2011 3:42 PM (permalink) Here is a link that does work: http://www.fws.gov/midwest/ashland/mtan_36.html#Hydrogen   #48 Umm_fish? Total Posts : 2835 Reward points : 953 Joined: 11/4/2009 Location: Boulder, CO, US Status: offline MBI: 775 Pnts MBI Site: Marine Aquarium Society of Colorado Re:Breeding Journal, Species: Liopropoma mowbrayi Tuesday, July 19, 2011 3:45 PM (permalink) Sorry. I meant to get back to you earlier but craziness ensued.   I used roughly 5mL of the generic peroxide from the drug store around the corner in about 750mL of salt water as a dip for about 30 minutes after harvest. Then I wash then into fresh salt water. --Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886   #49 Arc Katana Total Posts : 929 Reward points : 1293 Joined: 10/20/2009 Status: offline MBI Site: Marine Breeding Initiative (MBI) Re:Breeding Journal, Species: Liopropoma mowbrayi Tuesday, July 19, 2011 4:07 PM (permalink) Andy I assume that's the normal 35% stuff?   And thanks Duck!   #50 Umm_fish? Total Posts : 2835 Reward points : 953 Joined: 11/4/2009 Location: Boulder, CO, US Status: offline MBI: 775 Pnts MBI Site: Marine Aquarium Society of Colorado Re:Breeding Journal, Species: Liopropoma mowbrayi Tuesday, July 19, 2011 4:23 PM (permalink) I tracked down the bottle. It says 3% USP on it.   Basically, eggs withstand some pretty harsh environments. After hatch it's a different story, of course. So you want to be harsh off the bat but have it all gone by the time they hatch. --Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886   #51 Fishtal Total Posts : 5467 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Breeding Journal, Species: Liopropoma mowbrayi Tuesday, July 19, 2011 4:31 PM (permalink) Here is some info on H2O2: http://en.wikipedia.org/wiki/Hydrogen_peroxide   I use 20-40 volume H2O2 in my "day" job all the time. Since it oxidizes fairly quickly I don't see the point of a 24hr bath.  http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #52 Duck Total Posts : 466 Reward points : 465 Joined: 12/14/2010 Location: Barrow in Furness, Cumberland Status: offline MBI: 760 Pnts MBI Site: Marinefish-uk.co.uk Re:Breeding Journal, Species: Liopropoma mowbrayi Tuesday, July 19, 2011 4:52 PM (permalink) I would normally use it if i'm not sure of hatch date. I place the pot with eggs in a 2 litre container containg the H2O2 for 1 hour, before placing the pot in the BRT with the H2O2 water. As you sy Tal it oxidises fairly quickly and the only residue is oxygen and water.   #53 Arc Katana Total Posts : 929 Reward points : 1293 Joined: 10/20/2009 Status: offline MBI Site: Marine Breeding Initiative (MBI) Re:Breeding Journal, Species: Liopropoma mowbrayi Tuesday, July 19, 2011 5:49 PM (permalink) So here is a more formal outline of what I'm thinking of:   Collect eggs Eggs are in egg holder - h2o2 is added, sits for about 30 minutes. Eggs are then placed in new asw to "wash" them. Eggs collected via pipette and placed into hatching tank/tub/bucket etc with new ASW and heater.  Slow 1 or two bubbles a second via airstone.   Seems reasonable enough?   #54 Umm_fish? Total Posts : 2835 Reward points : 953 Joined: 11/4/2009 Location: Boulder, CO, US Status: offline MBI: 775 Pnts MBI Site: Marine Aquarium Society of Colorado Re:Breeding Journal, Species: Liopropoma mowbrayi Tuesday, July 19, 2011 6:51 PM (permalink) Pretty close to what I'm doing. I pipette into a small sieve already in the H2O2 bath. After about 30, I move the sieve to a clean salt bath, then I flush them into the hatching container. Witt tells me that you don't want any air at all. --Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886   #55 Arc Katana Total Posts : 929 Reward points : 1293 Joined: 10/20/2009 Status: offline MBI Site: Marine Breeding Initiative (MBI) Re:Breeding Journal, Species: Liopropoma mowbrayi Tuesday, July 19, 2011 6:58 PM (permalink) Now I'm tempted to get stuff set up lol!  Won't do me a whole lot of good if I'm gone at the conference till Monday tho!     Although if the prolarvae stage is as long as some say, it might be just in time to add copepods and iso to the hatch bucket....    #56 Umm_fish? Total Posts : 2835 Reward points : 953 Joined: 11/4/2009 Location: Boulder, CO, US Status: offline MBI: 775 Pnts MBI Site: Marine Aquarium Society of Colorado Re:Breeding Journal, Species: Liopropoma mowbrayi Wednesday, July 20, 2011 8:47 AM (permalink) Yeah, but it's so much fun to watch the process. --Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886   #57 Arc Katana Total Posts : 929 Reward points : 1293 Joined: 10/20/2009 Status: offline MBI Site: Marine Breeding Initiative (MBI) Re:Breeding Journal, Species: Liopropoma mowbrayi Friday, July 29, 2011 11:41 AM (permalink) So after getting back into the "groove" (40 hours of driving in 4 days will do that too you!) I collected some eggs last night.  Not a ton (20ish) as I forgot about it while watching Psych (oops!).  So I was all set to pick up some maroxy today (its for eggs after all!) until I got to reading whats in it - "stabilized oxides" aka its hydrogen peroxide!  So a quick run to Target got me two 32oz bottles for about two bucks (compared to the 9 bucks for 2oz of maroxy!)  I had the eggs in a holder with about 8oz of water from the tank.  This first batch is getting 1ml of peroxide for 30 minutes to see if that makes any difference in mortality.  Once they are done I'll give em a quick swirl in fresh saltwater and then plop em in some new saltwater (just shy of a gallon) that has 10 drops of peroxide in it.  I should have hatching in about 30-36 hours to see if it works.    #58 Arc Katana Total Posts : 929 Reward points : 1293 Joined: 10/20/2009 Status: offline MBI Site: Marine Breeding Initiative (MBI) Re:Breeding Journal, Species: Liopropoma mowbrayi Sunday, July 31, 2011 9:50 AM (permalink) Made the dumb mistake of putting them in a container with too many ridges - can't see anything!  I'm cleaning the tank today and moving the pair of clowns out to another tank.  This will make for some more room in the tank (going to remove some rock so better flow), as well as let me take the 3-4 gallons of water to start a new culture of the pods that are growing in there (I think its just some tisbe but maybe I can get more cyclopoids like I found before).     Once I get the 37 column tank up (20x18x24) I'll move them into that so I can deep clean the biocube.   #59 luis a m Total Posts : 451 Reward points : 1249 Joined: 3/15/2011 Location: Buenos Aires, Buenos Aires, AR Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Breeding Journal, Species: Liopropoma mowbrayi Sunday, July 31, 2011 2:28 PM (permalink) IME,and similarly others,prolarvae die after 2 days or so.This seems to happen with most species tried.Which is the reason of some sort of cooperative efforts. What is the cause and ;Wich is the idea of using peroxide?.Obviously,we suspect bacterial contamination.What you advise as an aproach?A starter dip and then keep an antibacterial agent in the rearing water?   #60 Online Bookmarks Sharing: Facebook Digg Google del.icio.us StumbleUpon Change Page: < 12345 > | Showing page 3 of 5, messages 41 to 60 of 85 - powered by ASPPlayground.NET Forum Trial Version

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