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•  Incubating Centropyge eggs and prolarvae.

  Change Page: < 1234567 > | Showing page 5 of 7, messages 81 to 100 of 131 - powered by ASPPlayground.NET Forum Trial Version Author Message zimajays Total Posts : 203 Scores: 4 Reward points : 351 Joined: 8/31/2010 Location: Lincoln, Lincolnshire, GB Status: offline MBI: 775 Pnts MBI Site: Marine Fish Breeders Club - UK Re:Incubating Centropyge eggs and prolarvae. Tuesday, November 8, 2011 12:13 PM (permalink) 0 I have used that method before when breeding neon tetras, we added a throat lozenger that had a probiotic something in.  Works well.  Its something I will be trying with the mandarin.  I sometimes wonder if our systems are too clean, and while bad bacteria is a problem in our effort to get rid of it we actually end doing more harm then good by killing the good stuff as well.  I think this is why it should be one or the other - sterile clean or very well matured.  Of course thats just me thinking! Proud member of the first dedicated breeders site in the UK Marine Fish Breeders Club - UK H.Zosterae - 60 DPS   #81 GreshamH Total Posts : 757 Scores: 16 Reward points : 600 Joined: 4/27/2011 Location: SF Bay Area, CA Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Incubating Centropyge eggs and prolarvae. Tuesday, November 8, 2011 1:53 PM (permalink) 0 Originally Posted by luis a m And Mindy,if you finished with the above,you should see this: http://www.oceanicinstitute.org/pdfs/Marine_Ornamentals_Research_a31979.pdf and I´m not sure if reading this is encouraging. A well known and well funded Institute,located in the best possible place. Staffed by experienced and well known researchers;Laidley,Shields,Chat. 19 tanks of 1,000 L each Parvo.cultures. Many,many thousand of eggs produced daily. Results of this project based on such a sheer set of means?:Some "dozens" of juvs,and not without some problems. How do we hobbyists stand in front of this? Papers don't always give you a full grasp of ones failure(s), nor do they put everything to print at times.  Do not let their failure get you down.    #82 luis a m Total Posts : 451 Scores: 2 Reward points : 1249 Joined: 3/15/2011 Location: Buenos Aires, Buenos Aires, AR Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Incubating Centropyge eggs and prolarvae. Thursday, November 10, 2011 10:38 AM (permalink) 0 That is the point ,Gresh.Failures are seldom published,and when they are,they are garnished with some convenient make-up.     #83 KathyL Total Posts : 2639 Scores: 13 Reward points : 1504 Joined: 6/6/2010 Location: St. Louis, MO, US Status: offline MBI: 1605 Pnts MBI Site: Marine Aquarium Societies of North America Re:Incubating Centropyge eggs and prolarvae. Friday, November 11, 2011 7:26 AM (permalink) 0 Originally Posted by Arc Katana One thing I totally forgot that WILL make a differnce:  Pro-biotics!  Yep, I mean those you can buy off the shelf.  I've cycled tanks with nothing but 2-3 capsules a day for a week.  Put fish in and everything is groovy.  There are a few papers out there dealing with it, mostly for the foodfish industry.   Basically its put the good stuff in, let it grow and hope it out competes the "bad" stuff.   If anyone feels the need to test it - please do!  Grab a 10G tank and drop 2-3 capsules a day for a week into the tank thats running any kind of filter and try it out.  Heck even if the tank is up and running it can make a difference.  Why?  Mostly because we usually are dealing with small volumes of water.  In a 100+ gallon system obviously you'd use a whole heck of a lot more, and have it take longer before results would show up too. Ill see if I can't find the papers and post em (or at least the abstract of them) after class tonight. Which probiotics, and where do you buy them? Healthfood store? Do you know the name of the bacteria?   #84 KathyL Total Posts : 2639 Scores: 13 Reward points : 1504 Joined: 6/6/2010 Location: St. Louis, MO, US Status: offline MBI: 1605 Pnts MBI Site: Marine Aquarium Societies of North America Re:Incubating Centropyge eggs and prolarvae. Friday, November 11, 2011 7:32 AM (permalink) 0 It would be helpful to know what didn't work.  Then we could try it ourselves and confirm that it didn't work.  Unfortunately, what worked or didn't work in one person's system will have the opposite effect in another person's system.    The queen of clownfish, and the resource and inspiration for many of us, Joyce Wilkerson, published that one should not use too much light on the clownfish larval tanks, and one needs to blacken the sides the larval tanks, techniques that I now completely disregard with great success.   <message edited by KathyL on Friday, November 11, 2011 9:37 AM>   #85 luis a m Total Posts : 451 Scores: 2 Reward points : 1249 Joined: 3/15/2011 Location: Buenos Aires, Buenos Aires, AR Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Incubating Centropyge eggs and prolarvae. Friday, November 11, 2011 8:45 AM (permalink) 0 There are two major factors that are common in all pro breeding projects (academic,public aquaria,commercial hatcheries) that can not be adopted by hobbyists: Very large larval tanks NSW The importance of which still remains unclear for me.   #86 EasterEggs Total Posts : 1946 Scores: 19 Reward points : 735 Joined: 9/22/2011 Location: Saskatoon, SK, CA Status: offline MBI: 590 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Incubating Centropyge eggs and prolarvae. Friday, November 11, 2011 9:14 AM (permalink) 0 I understand that Todd will be releasing some culture information for eggs, prolarvae, and larvae soon now that he has reached settlement, so hopefully he can clear some of this up for us.   #87 KathyL Total Posts : 2639 Scores: 13 Reward points : 1504 Joined: 6/6/2010 Location: St. Louis, MO, US Status: offline MBI: 1605 Pnts MBI Site: Marine Aquarium Societies of North America Re:Incubating Centropyge eggs and prolarvae. Friday, November 11, 2011 9:38 AM (permalink) 0 Wouldn't it be nice if he made reports here? Oh wait, we are on the wrong thread.  Todd raised basslets...   #88 WestOhooligan Total Posts : 82 Scores: 0 Reward points : 161 Joined: 11/9/2011 Location: Omaha, NE, US Status: offline MBI: 105 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Incubating Centropyge eggs and prolarvae. Friday, November 11, 2011 9:56 AM (permalink) 0 Originally Posted by luis a m There are two major factors that are common in all pro breeding projects (academic,public aquaria,commercial hatcheries) that can not be adopted by hobbyists: Very large larval tanks NSW The importance of which still remains unclear for me.   Forgive a noob, for stepping in here!  Luis, I am thinking if it is possible that NSW has some microbial/bacterial benefits that ASW does not?  That combined with the large larvae tanks may be detrimental to the growth of harmful bacteria?  I also wonder what temp they are keeping these tanks at as I know certain temps have different effects on bacterial growth.  Just my 2 cents.   #89 luis a m Total Posts : 451 Scores: 2 Reward points : 1249 Joined: 3/15/2011 Location: Buenos Aires, Buenos Aires, AR Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Incubating Centropyge eggs and prolarvae. Friday, November 11, 2011 10:30 AM (permalink) 0 Originally Posted by KathyL Wouldn't it be nice if he made reports here? Oh wait, we are on the wrong thread.  Todd raised basslets... Arc is spawninng basslets.Andy wrasses.But solving the prolarval mortality issue will be good for all of us,i think we are facing the same problem.   #90 luis a m Total Posts : 451 Scores: 2 Reward points : 1249 Joined: 3/15/2011 Location: Buenos Aires, Buenos Aires, AR Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Incubating Centropyge eggs and prolarvae. Friday, November 11, 2011 10:37 AM (permalink) 0 Originally Posted by WestOhooligan Forgive a noob, for stepping in here!  Luis, I am thinking if it is possible that NSW has some microbial/bacterial benefits that ASW does not?  That combined with the large larvae tanks may be detrimental to the growth of harmful bacteria?  I also wonder what temp they are keeping these tanks at as I know certain temps have different effects on bacterial growth.  Just my 2 cents. Yes,I thought about that,or even some unknown chemical factor.And I said above,I ran some tests with NSW,but no changes were found.   #91 EasterEggs Total Posts : 1946 Scores: 19 Reward points : 735 Joined: 9/22/2011 Location: Saskatoon, SK, CA Status: offline MBI: 590 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Incubating Centropyge eggs and prolarvae. Friday, November 11, 2011 11:12 AM (permalink) 0 Originally Posted by KathyL Wouldn't it be nice if he made reports here? Oh wait, we are on the wrong thread.  Todd raised basslets...   Prolarvae do as prolarvae are...?  Should be pretty close anyway.   #92 Umm_fish? Total Posts : 2835 Scores: 10 Reward points : 953 Joined: 11/4/2009 Location: Boulder, CO, US Status: offline MBI: 775 Pnts MBI Site: Marine Aquarium Society of Colorado Re:Incubating Centropyge eggs and prolarvae. Friday, November 11, 2011 12:36 PM (permalink) 0 From the website, it looks like Todd was facing some of the same problems we've faced. Hopefully he'll release some of his solutions.   http://www.reefs.com/blog/2011/11/10/another-milestone-in-aquaculture-first-rearing-of-the-reef-basslets-at-the-long-island-aquarium/ --Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886   #93 WestOhooligan Total Posts : 82 Scores: 0 Reward points : 161 Joined: 11/9/2011 Location: Omaha, NE, US Status: offline MBI: 105 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Incubating Centropyge eggs and prolarvae. Friday, November 11, 2011 3:44 PM (permalink) 0 Originally Posted by luis a m Yes,I thought about that,or even some unknown chemical factor...   It's interesting that you bring this up; we at the LFS were just having a conversation about this and the owner of the shop is convinced that some sort of chemical or hormone comes into play.   #94 luis a m Total Posts : 451 Scores: 2 Reward points : 1249 Joined: 3/15/2011 Location: Buenos Aires, Buenos Aires, AR Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Incubating Centropyge eggs and prolarvae. Thursday, November 17, 2011 10:47 AM (permalink) 0 Culturing in UV treated water now. On day 3,I removed about 100 dead PL.But other 100 are hanging in midwater and looking fine.Culture water remains Vibrio free.   #95 aomont Total Posts : 774 Scores: 0 Reward points : 1003 Joined: 2/12/2011 Location: Rio de Janeiro, RJ, BR Status: offline MBI: 5 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Incubating Centropyge eggs and prolarvae. Thursday, November 17, 2011 11:05 AM (permalink) 0 How big is the rearing tub Luis ? How many liters ? Anderson.   #96 luis a m Total Posts : 451 Scores: 2 Reward points : 1249 Joined: 3/15/2011 Location: Buenos Aires, Buenos Aires, AR Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Incubating Centropyge eggs and prolarvae. Thursday, November 17, 2011 11:40 AM (permalink) 0 Originally Posted by aomont How big is the rearing tub Luis ? How many liters ? Not rearing tubs so far,Anderson Eggs are hatched in trays and PL are moved to culture dishes,the size of a Petri dish,but taller.   #97 aomont Total Posts : 774 Scores: 0 Reward points : 1003 Joined: 2/12/2011 Location: Rio de Janeiro, RJ, BR Status: offline MBI: 5 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Incubating Centropyge eggs and prolarvae. Thursday, November 17, 2011 7:09 PM (permalink) 0 Huumm, so you are adding ~200 eggs to which amount of water ? And what density of PL you have after you move them ? Anderson.   #98 EasterEggs Total Posts : 1946 Scores: 19 Reward points : 735 Joined: 9/22/2011 Location: Saskatoon, SK, CA Status: offline MBI: 590 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Incubating Centropyge eggs and prolarvae. Thursday, November 17, 2011 7:36 PM (permalink) 0 That sure is a small amount of water Luis.  I'm thinking (in raw newbie terms btw) is to put the eggs in something like a rotifer sieve from FAF, the one in a 4" pipe?  Then clip that to the side of a 10 or 20 gallon tank with a heater and a MiniJet pump...?  Then don't transfer them, they can stay as PL in the rotifer sieve...albeit I think you would want a smaller sieve so the copepods can't escape.   #99 luis a m Total Posts : 451 Scores: 2 Reward points : 1249 Joined: 3/15/2011 Location: Buenos Aires, Buenos Aires, AR Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Incubating Centropyge eggs and prolarvae. Thursday, November 17, 2011 10:30 PM (permalink) 0 Anderson,the dishes hold 150 ml,which gives an initial density of more than 1PL/ml. Mindy,the amount of water is irrelevant because it is being exchanged at the rate of 1 drop/sec.,or a complete turnover every 50 min.This kind of container works well for experimental work,as they can be checked with a microscope. And they are in a rot sieve which sits in the dish!53 mic mesh can hold most naups,something smaller will clog fast. The purpose of this project is to solve the problem of prolarval mortality.Raising angels is far,very far. Today in their 4th day,there were less deaths,about 20%.PL are well developed,with refringent eyes,many resting on the bottom,but others swim in midwater.Development seems faster,may be the temp is a bit higher. Vibrio showed but at very low numbers:3 CFU.   #100 Online Bookmarks Sharing: Facebook Digg Google del.icio.us StumbleUpon Change Page: < 1234567 > | Showing page 5 of 7, messages 81 to 100 of 131 - powered by ASPPlayground.NET Forum Trial Version

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