Culture Journal, Species: Nanochloripsis

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rgrking
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Culture Journal, Species: Nanochloripsis - Thursday, May 5, 2011 5:06 PM
Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system

General
Species:  Nanochloripsis
Species description:  micro algae
Culture source (link if possible):  http://florida-aqua-farms.com/secure/agora.cgi
If algae, CCMP # (Optional): 
http://ccmp.bigelow.edu/
Culture Establishment Date:  04/30/2011
Continuation Date:  08/25/2011

Culturing Vessel Details
Salinity:  1.022
Temperature:  72F 
pH:  8.0

Vessel description:  2 liter bottle
Lighting description:  100W CFL
Lighting cycle:  Continuous
Aeration description: rolling bubble (4-5 per second)

Methodologies
Split methodology: 
I'm only splitting when the color is a dark green and it's a full bottle. I've lost cultures by splitting to soon and adding to much water to them.

Culture medium description: 
Micro Algae Grow from Florida Aqua Farms 0.5mL each day for full 2L bottle

Cell count:
  unknown

Reference links: 

http://reefkeeping.com/issues/2002-07/ds/
https://srac.tamu.edu/ind...et/whichfactsheet/224/
ftp://ftp.fao.org/docrep/..003/w3732e/w3732e00.pdf

Additional Information
(No Pictures or Videos in the Section Please)
Notes:  I sterilize by filling the bottle with a cap full of bleach and the rest is RO water. Rinse till the bleach smell is gone and let it dry for a day. 
 
I'm not sure why, but some of the bottles I have lost have looked great one day and turn light green, then brown, then clear. I'm dosing and adding top off the same in each bottle. Any ideas would be great!



You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos  and information the MBI Council will not be able to approve your reports.

<message edited by rgrking on Friday, October 14, 2011 10:04 PM>
RLTW

180 Gallon Mixed Reef

Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8

rgrking
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Re:Nanochloripsis - Thursday, May 5, 2011 5:13 PM
taken 4/30/11

taken 5/1/11


taken 5/2/11

RLTW

180 Gallon Mixed Reef

Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8

THEJRC
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Re:Nanochloripsis - Thursday, May 5, 2011 7:01 PM
lookin good!!  you may want to drop your salinity a few points as the culture will be easier to work with (1.020 ish is typically good). 
Pelagically yours,
~J      

rgrking
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Re:Nanochloripsis - Thursday, May 5, 2011 7:03 PM
thanks for the advice! when I divide it I'll lower it then
RLTW

180 Gallon Mixed Reef

Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8

rgrking
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Re:Nanochloripsis - Thursday, August 25, 2011 2:48 PM
updated pics of culture. I have 3 2L bottles going that are full
 

 

 

 
 
RLTW

180 Gallon Mixed Reef

Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8

rgrking
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Re:Nanochloripsis - Thursday, August 25, 2011 3:01 PM
Here is what I made to distribute the air.
 

RLTW

180 Gallon Mixed Reef

Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8

KathyL
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Re:Nanochloripsis - Friday, August 26, 2011 6:50 AM
looks great!