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                            Culture Journal, Species: Brachionus plicatilis
                        
                        
                            Thursday, May 5, 2011 5:19 PM
                        
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                      Culturing Journal DataSheet     This first post should be updated regularly to include new information as events take place or changes are made to your system     General     Species:           Brachionus plicatilis        Species description:   small 130-340 µm long         Culture source (link if possible ):           http://florida-aqua-farms.com/secure/agora.cgi    If algae, CCMP # (Optional ):            http://ccmp.bigelow.edu/    Culture Establishment Date:  05/04/2011        Continuation Date:       8/4/2012       Culturing Vessel Details     Salinity:           1.020        Temperature:     72F        pH:  8.0          Vessel description:  5 gallon bucket     Lighting description:  AMBIENT    Lighting cycle:  ON AT 1000 OFF AT 2100    Aeration description:  LARGE SLOW ROLLING BUBBLES        Methodologies     Split methodology:  take half and pour into another bucket        Culture medium description:  RGComplete         Cell count:     (if known)                     Reference links:                  http://en.wikipedia.org/wiki/Brachionus_plicatilis   http://www.advancedaquarist.com/2002/9/breeder   http://www.mariculturetechnology.com/Rotifer.PDF   http://www.scielo.sa.cr/s...amp;script=sci_arttext   Additional Information     (No Pictures or Videos in the Section Please)    Notes:                           You will be required to provide photographic evidence and as much detail as possible about your project in this thread.                  If your thread does not contain detailed enough photos  and information the MBI Council will not be able to approve your reports.   
                    <message edited by rgrking on Tuesday, July 17, 2012 9:00 AM> 
                    
                 
                
                     RLTW          180 Gallon Mixed Reef       Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8   
                  
             
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                            Re:Culture Journal, Species: Brachionus plicatilis
                        
                        
                            Thursday, May 5, 2011 5:56 PM
                        
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                     RLTW          180 Gallon Mixed Reef       Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8   
                  
             
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                            Re:Culture Journal, Species: Brachionus plicatilis
                        
                        
                            Wednesday, May 11, 2011 9:22 PM
                        
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                      Are you having good luck using the 2L bottle for your rotifers? I find that white 5G buckets work really well. Also, rotifers don't need light, you can save a little $ by not lighting them.      
                    
                    
                 
                
             
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                            Re:Culture Journal, Species: Brachionus plicatilis
                        
                        
                            Wednesday, October 19, 2011 2:33 PM
                        
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                              video coming soon. This is the strongest magnification I have.   
                    
                    
                  
                
                     RLTW          180 Gallon Mixed Reef       Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8   
                  
             
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                            Re:Culture Journal, Species: Brachionus plicatilis
                        
                        
                            Wednesday, October 19, 2011 4:09 PM
                        
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                     RLTW          180 Gallon Mixed Reef       Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8   
                  
             
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                            Re:Culture Journal, Species: Brachionus plicatilis
                        
                        
                            Tuesday, July 17, 2012 7:47 AM
                        
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                     RLTW          180 Gallon Mixed Reef       Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8   
                  
             
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                            Re:Culture Journal, Species: Brachionus plicatilis
                        
                        
                            Tuesday, July 17, 2012 11:44 AM
                        
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                     RLTW          180 Gallon Mixed Reef       Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8   
                  
             
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                            Re:Culture Journal, Species: Brachionus plicatilis
                        
                        
                            Tuesday, July 17, 2012 6:37 PM
                        
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                     RLTW          180 Gallon Mixed Reef       Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8   
                  
             
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                            Re:Culture Journal, Species: Brachionus plicatilis
                        
                        
                            Tuesday, July 17, 2012 7:18 PM
                        
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                      I see tons of pods....but where are the rotifers?    
                    
                    
                 
                
                    
                 
             
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                            Re:Culture Journal, Species: Brachionus plicatilis
                        
                        
                            Tuesday, July 17, 2012 7:36 PM
                        
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                      I can't get em to slow down. I need to try to strain some out.    
                    
                    
                 
                
                     RLTW          180 Gallon Mixed Reef       Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8   
                  
             
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                            Re:Culture Journal, Species: Brachionus plicatilis
                        
                        
                            Tuesday, July 17, 2012 7:38 PM
                        
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                      plus I don't have a microscope. only a little play one that plugs into a usb         
                    
                    
                 
                
                     RLTW          180 Gallon Mixed Reef       Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8   
                  
             
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                            Re:Culture Journal, Species: Brachionus plicatilis
                        
                        
                            Sunday, July 22, 2012 7:24 PM
                        
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                      one of them finally loaded. This is sieved through 53um and caught with a 25um screen.           
                    
                    
                 
                
                     RLTW          180 Gallon Mixed Reef       Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8   
                  
             
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                            Re:Culture Journal, Species: Brachionus plicatilis
                        
                        
                            Wednesday, July 25, 2012 8:27 AM
                        
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                     RLTW          180 Gallon Mixed Reef       Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8   
                  
             
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                            Re:Culture Journal, Species: Brachionus plicatilis
                        
                        
                            Wednesday, August 29, 2012 8:05 PM
                        
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                      I'm going to get a new culture started on this. I had a video that I saw some in, then it wouldn't upload. Then I took a shorter one, and couldn't make any out. Here it is. If you see any then let me know. If not, no biggie. Got a new start coming.           
                    
                    
                 
                
                     RLTW          180 Gallon Mixed Reef       Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8   
                  
             
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                            Re:Culture Journal, Species: Brachionus plicatilis
                        
                        
                            Sunday, September 2, 2012 9:57 PM
                        
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                      What you have there is a fantastic culture of probably Apocyclops panamensis, since you got rotifers from me.  They got in my rotifer culture, and are thriving there.  Also, if you got them from me any rotifers are not plicatilis, they are rotundiform.  Sorry.        Rotifers are smaller and move with a wandering, not a darting motion.   
                     <message edited by KathyL on Sunday, September 2, 2012 11:58 PM> 
                    
                 
                
                     check out Kathy's Clowns, llc website:    http://kathysclowns.com   Captive bred clownfish and more    (Wholesale to the trade.)   
                  
             
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                            Re:Culture Journal, Species: Brachionus plicatilis
                        
                        
                            Wednesday, September 5, 2012 5:44 PM
                        
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                      I know. Thanks for the update. I'm going to filter these out and add them to the tanks. I'm ordering a new start this week for the rotifers. If you need anything let me know!    
                    
                    
                 
                
                     RLTW          180 Gallon Mixed Reef       Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8   
                  
             
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                            Re:Culture Journal, Species: Brachionus plicatilis
                        
                        
                            Wednesday, September 5, 2012 7:32 PM
                        
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                      Likewise.    
                    
                    
                 
                
                     check out Kathy's Clowns, llc website:    http://kathysclowns.com   Captive bred clownfish and more    (Wholesale to the trade.)   
                  
             
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                            Re:Culture Journal, Species: Brachionus plicatilis
                        
                        
                            Sunday, September 30, 2012 1:07 PM
                        
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                     RLTW          180 Gallon Mixed Reef       Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8   
                  
             
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