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Re:Supplies you need to culture O. marina
Sunday, August 14, 2011 3:32 PM
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Just let me know how it goes and I'll pay for the shipping and whatever else.
RLTW 180 Gallon Mixed Reef Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8
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Re:Supplies you need to culture O. marina
Sunday, August 14, 2011 3:46 PM
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Preliminary shipper temp experiment results: I put two, 1-liter bags of 70F water (one with a thermometer inside) into a standard Seahorse Source styro shipper, placing the bags on a 1" bed of styro peanuts, and then filling the rest of the shipper with more styro peanuts. Taped the cardboard box shut. Put the box in the back of my Volvo wagon that had been sitting in the sun all day, with the windows rolled up. Ambient temp. outside today has ranged from 75-80F in the shade. The temp in the car has ranged from 95F to 115F (!) over the last 3+ hours. It has been over 100F for more than 2 hours, more like 3 hours. I just pulled the package out, and the outside of it was VERY HOT to the touch (115F). The thermometer read 76F. I closed it all back up, and put it back into the hot car, and will check on it again later.
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Re:Supplies you need to culture O. marina
Sunday, August 14, 2011 4:44 PM
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 Originally Posted by
can I just drive over there and pick some up? maybe actually make good on that pizza I owe you..... Sure. When you want to do that? Come up on a Friday then stop back by the salami place on the way home. You won't regret it.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Supplies you need to culture O. marina
Sunday, August 14, 2011 6:17 PM
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Heck, for that matter, Joe, I can just make up a salami sampler for you whenever you'd like to come by. It'll be gooooooddddd......
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Supplies you need to culture O. marina
Sunday, August 14, 2011 6:18 PM
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As the sun moved westward, and more direct sunlight was coming in the passenger side windows (car was facing south), that combined with the reflected heat off of the white garage door to the east brought the temperature, in the shade, inside the car to over 130F! The box had sat, in the shade (no direct sunlight hitting the box in the car), in temperatures over 120F for at least another hour. The thermometer in the water in the bag now reads 80F. I think it's safe to say that shipping overnight this time of year is quite acceptable, IMHO. I subjected the box to conditions MUCH more extreme than any box in an open UPS or FedEx truck would be subjected to. Total exposure was to temperatures well over 100F, reaching 130F, for over 5 hours (more like 6 hours), with a total rise in internal temp from 70F to 80F only.
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Re:Supplies you need to culture O. marina
Sunday, August 14, 2011 8:47 PM
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Re:Supplies you need to culture O. marina
Thursday, August 18, 2011 1:04 PM
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ok. just ordered a couple of the sheets of 27um mesh. I still need to get an O. marina and it's food. Does anyone have a link for what to feed them? Any idea on the cost? Can it be cultured?
RLTW 180 Gallon Mixed Reef Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8
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Re:Supplies you need to culture O. marina
Thursday, August 18, 2011 2:16 PM
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Roti-Grow Plus and N-Rich (together with ClorAm-X) have been used before. They are Reed Mariculture products. You can, of course culture live phyto to feed O. marina, but if you're successful at doing that, it begs the question of whether you need O. marina or not!
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Re:Supplies you need to culture O. marina
Thursday, August 18, 2011 2:18 PM
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 Originally Posted by JimWelsh
You can, of course culture live phyto to feed O. marina, but if you're successful at doing that, it begs the question of whether you need O. marina or not! Eh dont think of it as a phyto replacement but a phyto enrichment... one can take the easier standards such as nanno and tet.... feed the O marina... and bang... something with movement and a different size / flavor
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Re:Supplies you need to culture O. marina
Thursday, August 18, 2011 3:12 PM
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Which is why I said "begs the question". One can probably raise many species of copepods just fine with the right phytoplankton(s), so perhaps it is not necessary to raise O. marina to feed your copepods if you are successful at raising the right microalgae. But then again, perhaps there is something to "trophic upgrading", and even though it is not necessary, it may still be beneficial to raise O. marina, too.
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Re:Supplies you need to culture O. marina
Thursday, August 18, 2011 5:39 PM
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I have a good culture of nanno going right now. I have 4 full 2L bottles and should have 6 by the weekend. I'm getting ready to start up another rotifer culture though. I guess I may need to put off the rotifers and make some more nanno before I get going to strong for my pod culture.
RLTW 180 Gallon Mixed Reef Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8
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Re:Supplies you need to culture O. marina
Thursday, August 18, 2011 6:13 PM
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Jim--Sorry I never got back to this. Thanks for doing the shipping experiment. I was trying to keep people's costs down so I was trying to wait until we could go with less packaging (since these are quite hardy critters). But if people want to do the shipments sooner, let me know. We just had the hottest day of our summer today, but it's supposed to moderate a little. rgrking--I do not know if you can maintain an O. marina or Apocyclops culture on nanno alone. I would tend to doubt it, personally. Then again, the O. marina can survive on RotiGrow+, which is based on nanno as far as I can tell, so it's possible. They don't do nearly as well as I would like on RG+ alone, but it's certainly something to experiment with.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Supplies you need to culture O. marina
Friday, August 19, 2011 8:19 AM
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hmm. What do you feed them then? Would I need to start a T iso culture? I'm not sure I can maintain the temp that it needs.
RLTW 180 Gallon Mixed Reef Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8
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Re:Supplies you need to culture O. marina
Friday, August 19, 2011 8:34 AM
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What do you mean by "them"?
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Supplies you need to culture O. marina
Friday, August 19, 2011 9:38 AM
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RLTW 180 Gallon Mixed Reef Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8
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Re:Supplies you need to culture O. marina
Friday, August 19, 2011 6:52 PM
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We're still consistently around 107 - 108 F here, so I'm waiting a bit longer to ask to ship this way. In the meantime I'm working on my rotifer culture and trying to figure out how to transfer containers without making a mess.
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Re:Supplies you need to culture O. marina
Tuesday, August 23, 2011 9:56 AM
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I got my 27um sheets in yesterday. I can use them for this project and for my shrimp breeding. good times there! lol
RLTW 180 Gallon Mixed Reef Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8
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Re:Supplies you need to culture O. marina
Wednesday, August 24, 2011 10:39 PM
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And 108 in Denton is like 115 anywhere else.  I used to live on Oak Street, 20 years ago. I heard they just tore my old house down over there this summer. Sorry I haven't been around. Work is ugly right now. All the authors are dumping work on me like crazy. It's always like this at the end of the summer.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Supplies you need to culture O. marina
Wednesday, August 24, 2011 10:41 PM
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--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Supplies you need to culture O. marina
Wednesday, August 24, 2011 11:24 PM
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Sure thing. Makes sense that if O marina can make DHA out of lesser alga, that that may be why we have such success when we feed it to copepods.
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