Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Oxyrrhis Marina
Species description: dinoflagellate
Culture source (link if possible
): Jim Welsh, kind donation.
If algae, CCMP # (Optional
):CCAP# 1133/3 Oxyrrhis marina Dujardin
http://www.algaebase.org/...tail/?species_id=52504 http://ccmp.bigelow.edu/ Culture Establishment Date: 6 /8/2011
Continuation Date: Culturing Vessel Details Salinity: 20ppt
Temperature: ambient
pH: 8ish
Vessel description: plastic jars of various sizes, gallon and half gallon, and 5 gallon buckets
Lighting description: fluorescent grow bulb on timer
Lighting cycle: 14 day , 10 night
Aeration description: open airline, few bubbles per second, rapid but not boiling
Methodologies Split methodology: various. I've previously kept them going with just feeding and weekly additions of saltwater. And then they crashed. Now wish to try more dynamic method. Daily removal of part of the culture, about 1/8 initially, replacement with 2 x the volume removed of clean saltwater, 20ppt. Feeding twice daily.
Culture medium description: Bleached saltwater is dechlorinated with excess chloramX and RotiGrow + and or N-Rich added as need to maintain or grow culture. Successful method follows:
Cell count: I am not able to count them. Qualitative observations ? I can see them swimming in the 40x objective of the scope.
Reference links: http://www.int-res.com/ar.cles/ame/10/a010p307.pdf Grazing in the heterotrophic dinoflagellate Oxyrrhis marina: size selectivity and preference for calcified Emiliania huxleyi cells http://www.algaebase.org/...tail/?species_id=52504 Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by KathyL on Tuesday, August 23, 2011 9:48 PM>