Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Brachionus plicatilis
Species description: Rotifers, L strain
Culture source (link if possible
): Reed Mariculture
If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 1/3/2013
Continuation Date: Culturing Vessel Details Salinity: 1.020
Temperature: ambient room temp. ~79 F
pH: 7.7
Vessel description: 5 gallon orange plastic buckets (from Home Depot)
Lighting description: ambient room light, but culture buckets are covered with a lid to keep out cats
Lighting cycle: mostly natural day cycle from ambient room lighting
Aeration description: slow boil
Methodologies Split methodology: Culture medium description: new saltwater, mixed at least 24 hours prior to 1.025, and then diluted down to 1.020
Cell count: At culture start, it was ~1 million rotifers.
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: This is the third culture journal attempt. The first culture never really got started. The second ran successfully from 9/11 - 12/11 and crashed from neglect while I had the flu.
You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by gramalkin on Monday, January 28, 2013 12:07 PM>