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Culture Journal, Species: Isochrysis galbana (C-ISO)
Thursday, August 18, 2011 1:32 PM
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Culturing Journal DataSheet This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Isochrysis galbana (C.iso) Species description: Brown Phytoplankton Culture source (link if possible ): http://www.seahorsesource.com/cgi-bin/shop/search.cgi?&category=Foods-Algaes If algae, CCMP # (Optional ): http://ccmp.bigelow.edu/ Culture Establishment Date: 8/18/2011 Continuation Date: on going Culturing Vessel Details Salinity: 1.018 Temperature: Unregulated Basement Room Temperature (70-80s) pH: Untested. Newly mixed Instant Ocean Salt. Vessel description: 2L Soda bottle. Right after I finish drinking the soda, I rinse it a few times and soak it with tap water for days until whenever I'm about to use it. When I need it, I rinse it with tap water few more times then fill with RO water and salt. I do not sterilize them. Lighting description: 4 tubes of Home Depot 3' T-5 light combo. The color looks like 3000K. Lighting cycle: 18 hours a day Aeration description: 1 so fast like boiling and another 1 bubbling a bit slower at a rate that is is possible to count. Methodologies Split methodology: Pour out 1/2 of the bottle for feeding. Pour another 500ml into a new bottle for splitting to another culture. When I split and create a new culture, I only use new bottles for the new culture. For the original dense culture bottle, after I pour out the amount that I use, I just refill it with salt water. The original bottle will be keep re-using until there are stuff or film developing near the bottle neck, then I just trash the bottle (by putting them into the city recycle bin. Not really trashing it). I do not clean or sterilize any them. Culture medium description: Just poured the source culture into the salt water (already mixed with fertilizer) in the soda bottle. Put on the drill cap and air tube. Cell count: unknown Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread. If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by chuenwe on Friday, September 9, 2011 1:42 PM>
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Re:Culture Journal, Species: Isochrysis galbana (C-ISO)
Thursday, August 18, 2011 8:09 PM
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My bubbling rate for the nano and tetra are like boiling. Should I use much lower bubbling rate for ISO? The light color ones are the new C-ISO culture.
<message edited by chuenwe on Thursday, August 18, 2011 10:07 PM>
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Re:Culture Journal, Species: Isochrysis galbana (C-ISO)
Thursday, August 18, 2011 9:39 PM
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I use a low boil for my Isochrysis. Not a full roiling boil, but not a simmer either. If in doubt, use more air rather than less, IMHO.
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Re:Culture Journal, Species: Isochrysis galbana (C-ISO)
Thursday, August 18, 2011 10:06 PM
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Thank you! Let me turn it back up a bit.
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Re:Culture Journal, Species: Isochrysis galbana (C-ISO)
Saturday, August 27, 2011 3:06 PM
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It's been 7 days. I'm going to split the bottom one either today or tomorrow.
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Re:Culture Journal, Species: Isochrysis galbana (C-ISO)
Saturday, August 27, 2011 3:51 PM
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I would wait a little longer. For me, after about 10 days it usually gets a dark chocolate brown. That's when I usually split.
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Re:Culture Journal, Species: Isochrysis galbana (C-ISO)
Saturday, August 27, 2011 5:06 PM
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ah, ok. Thank you. Let me do that as well. When you split, do you usually split it into 2 bottle or 3?
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Re:Culture Journal, Species: Isochrysis galbana (C-ISO)
Saturday, August 27, 2011 5:12 PM
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Each split is 500 ml of dense culture added to 1500 ml of fresh media. I usually make 2 splits (1000 ml of dense culture used to make 2 x 500 ml splits) each time, but sometimes only 1. The remaining 1000 - 1500 ml of dense culture gets fed to things.
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Re:Culture Journal, Species: Isochrysis galbana (C-ISO)
Saturday, August 27, 2011 5:38 PM
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Ah, I see. I'll do the same then.
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Re:Culture Journal, Species: Isochrysis galbana (C-ISO)
Sunday, August 28, 2011 1:09 PM
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Sorry to hijack the thread here but iso is my nemesis... Jim, you noted splitting oa 10 day schedule, is this your typical? I tend to stick with a rigid 8 day for most of my cultures allowing me to easily pipeline daily supply with 9 cultures (1 culture split each day and a 9 th culture maintained as a fallback.) Given a 10 day schedule this means 11 cultures using my methodology. Have you done a shorter split cycle with any luck?
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Re:Culture Journal, Species: Isochrysis galbana (C-ISO)
Sunday, August 28, 2011 1:27 PM
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No problem at all. I like to know as well. I usually don't even do the split method. I use 1/4-1/3 of it and refill it with fresh media. Then I'll use it again when the color looks right, usually within just a few days. This splitting method is totally new to me.
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Re:Culture Journal, Species: Isochrysis galbana (C-ISO)
Sunday, August 28, 2011 1:48 PM
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Yes, I have been able to split sooner with success. My philosophy is to split near, but beforee, the end of the exponential growth phase. Additionally, the cultures are at their peak nutritional value for feeding out. To me, it just makes sense that the denser the culture I'm splitting (within reason), the healthier it is, and it will more likely be able to "win out" over any few contaminate cells that make it in.
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Re:Culture Journal, Species: Isochrysis galbana (C-ISO)
Sunday, August 28, 2011 1:49 PM
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The scheduled splits I took from Hoff (a hero I guess) I remember early on harvesting based on color which worked well for nano but I had a lot of failure with other species. It was not until I started my regimented controls (as Randy Reed stated you have to run it as a factory) that I noticed my failure rate drop and success with other species work. Sadly, with all the species I have done iso has always been the most difficult for me, I suspect temperature but also suspect longer times for the culture to reach log state. Jim cultures iso as second nature so I am of course always curious as to what he is doing different!
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Re:Culture Journal, Species: Isochrysis galbana (C-ISO)
Wednesday, August 31, 2011 1:42 PM
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I don't know how i count the date but few days ago I thought it was the 7th day but it was wrong... lol Today is already the 13th day. The color is a little darker then the above picture. Not dark chocolate brown at all. I just splited the bottom bottle that had more starting culture to begin with. 1L went to feed stuff. The rest of 1L went into 2 bottles and filled the bottle up with 1.018 clean salt water.
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Re:Culture Journal, Species: Isochrysis galbana (C-ISO)
Wednesday, August 31, 2011 1:50 PM
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You were correct to split today then. Be patient. It can take a while for the culture to acclimate to your environment and habits. How are you measuring your F/2?
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Re:Culture Journal, Species: Isochrysis galbana (C-ISO)
Wednesday, August 31, 2011 1:54 PM
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I premake clean salt water in lot of 2L bottles. I put 15-20 drops of F/2 into one bottle that I'm about to use.
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Re:Culture Journal, Species: Isochrysis galbana (C-ISO)
Wednesday, August 31, 2011 2:16 PM
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I took this picture after I split the bottom bottle.
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Re:Culture Journal, Species: Isochrysis galbana (C-ISO)
Thursday, September 1, 2011 7:38 PM
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1 day after the split. Lot of dark brown color algae like stuff sticking on the bottle wall. They are like hair algae waving around by the air bubbles. Never had that before.
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Re:Culture Journal, Species: Isochrysis galbana (C-ISO)
Sunday, September 4, 2011 1:58 PM
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I have seen something like this before. I've had cultures recover from this without issue. I usually turn the air down a bit if I see this. Now that it's been a few more days, how does this culture look?
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Re:Culture Journal, Species: Isochrysis galbana (C-ISO)
Sunday, September 4, 2011 2:28 PM
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It doesn't seem to have any different in the last few days. I thought it got darker, but after I took a picture and compare to the one posted, they are about the same color.
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