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Culture Journal, Species: Apocyclops panamensis
Saturday, August 20, 2011 6:47 PM
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Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Apocyclops panamensis Species description: egg carrying cyclopoid Culture source (link if possible ): Kind gift of Jim Welsh, arrived 8/12/2011 via Fedex, room temp. If algae, CCMP # (Optional ): http://ccmp.bigelow.edu/ Culture Establishment Date: 8/12/2011 Continuation Date: 11/12/2011 Culturing Vessel Details Salinity: 20 ppt Temperature: 75 pH: 8.2
Vessel description: One gallon square jar with wide mouth lid, hole in center, rigid airline connected to pump Lighting description: 5500K fluorescent bulb, Lighting cycle: 14 day/ 10 night Aeration description: rapid, but not boiling Methodologies Split methodology: Not sure yet, just trying to get a dense culture. Weekly, decant culture to clean jar. Culture medium description: Food is 6 parts N-Rich, 1 part Roti-Grow+ (6NR/1RG+). 6 drops per feeding, 2 feedings per day. Equal volume of CloramX at each feeding. Starting at week 2, 1/2 cup of O. marina culture added per day as well. Cell count: Not known. (if known) Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by KathyL on Monday, November 14, 2011 9:02 PM>
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Re:Culture Journal, Species: Apocyclops panamensis
Saturday, August 20, 2011 7:04 PM
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Today I did the first jar change. I decanted the culture into a clean jar and suspended the sediment and poured that into a couple of tall tubes. I let that settle and took a video of the little twitchers: 
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Re:Culture Journal, Species: Apocyclops panamensis
Saturday, August 20, 2011 7:08 PM
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Re:Culture Journal, Species: Apocyclops panamensis
Saturday, August 20, 2011 7:12 PM
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after the tubes' sediment settled, and after I took video and pictures, I decanted the pods back into the culture, reserving the sediment to the trash. I am really suprised at the density of this culture. What I thought was detritis, was really pods in suspension.  There are significant numbers of ciliates in this culture, but they appear to do no harm.
<message edited by KathyL on Sunday, August 21, 2011 6:13 AM>
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Re:Culture Journal, Species: Apocyclops panamensis
Saturday, August 20, 2011 9:02 PM
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Looking good, Kathy! I see lots of gravid females in the video. Was the video taken today?
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Re:Culture Journal, Species: Apocyclops panamensis
Sunday, August 21, 2011 6:12 AM
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Yes, it was taken the day it was posted, which is yesterday, now. I put some sediment in one of the tubes you've sent me, and then took the video directly from that after it settled. This culture has done remarkably well, so far. Let's see if I can keep from crashing it.
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Re:Culture Journal, Species: Apocyclops panamensis
Sunday, August 21, 2011 6:16 AM
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Jim, do you have ciliates in your culture? Or did I acquire them here?
When will we be able to get Culture points for this species? I am surprised it is not already accepted at the MBI!
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Re:Culture Journal, Species: Apocyclops panamensis
Sunday, August 21, 2011 12:58 PM
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So, the fact that you have nice gravid females means your feeding protocol is working well! I probably do have ciliates in my culture. I've submitted a classification request for this species, but so far, it has not been approved yet.
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Re:Culture Journal, Species: Apocyclops panamensis
Sunday, August 21, 2011 6:00 PM
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I was concerned about the amount of detritus in this culture, so I turned the air off for a couple of hours to get it all to settle. The copepods continue to swim in the water body.
I decatanted the square jar and got a nice clean culture from it. Square jars are easy to decant, because one can just tip it into the new clean jar, until it is sitting on its nice flat side. The detritus stays in, and the majority of the culture is in the new jar.
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Re:Culture Journal, Species: Apocyclops panamensis
Tuesday, August 23, 2011 6:17 PM
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Here's the culture shelf: 
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Re:Culture Journal, Species: Apocyclops panamensis
Friday, August 26, 2011 5:35 AM
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Now there are strands of detritus in the jar. It's Friday, and I'll decant again on the weekend. Culture looks cloudy, and i wonder if there are bacteria in it. Could it be the O marina?
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Re:Culture Journal, Species: Apocyclops panamensis
Friday, August 26, 2011 10:51 AM
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Decant some off into a clear glass container (I would use something like a 250 ml Erlenmeyer flask) and let it sit for a few minutes. Shine a flashlight diagonally from the back (flashlight at 2 or 3 o'clock, with your eyes at 6 o'clock, if that makes sense) and use a magnifying glass to examine it. If your magnifying glass is strong enough, the O. marina will be little specks or "sparkles" swimming around. Bacteria will just be cloudy still. It also could just be tons of nauplii. A dense culture can look cloudy when bubbling.
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Re:Culture Journal, Species: Apocyclops panamensis
Friday, August 26, 2011 9:49 PM
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I took a pudding cup's worth of culture, put it thru a 37 micron, back washed into a petrie dish (6 cm diameter): CLICK on the video 
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Re:Culture Journal, Species: Apocyclops panamensis
Friday, August 26, 2011 9:51 PM
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Thanks, Jim, I'll try it tomorrow.
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Re:Culture Journal, Species: Apocyclops panamensis
Sunday, September 4, 2011 3:50 PM
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Jim, that 2 oclock, 6 oclock light angle is brilliant! It really helps to see the culture.
A. panamensis cultures are doing well, better than anything besides O. marina. They have outcompeted T. californicus in the polyglot bucket, as well as doing pretty well in the simple culture with O. marina. I have O. marina everywhere, and I almost have A. panamensis everywhere. Not sure which culture is more successful, the one that I fuss over, or the bucket on the floor that gets all the leftover pods and rots, with just feeding and little or no water change.
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Re:Culture Journal, Species: Apocyclops panamensis
Tuesday, September 13, 2011 8:18 PM
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After a couple of days of starving the culture during MACNA, I've discovered that precious few A. panamensis are still alive in the single culture, while they are still thriving in the polyglot bucket on the floor, and I haven't looked under the microscope, but there seems to be a healthy population outside as well. I've sieved them out of the water of the single culture, and given them clean saltwater, a dose of O. marina, and some of Andy's recipe phyto. I hope they bounce back.
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Re:Culture Journal, Species: Apocyclops panamensis
Wednesday, September 28, 2011 7:26 AM
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They are not bouncing, though they appear to still be there. I feed them sparsely, and hope.
The outdoor culture, that was just seeded with rotifers and A. panamensis is really doing well. It's 62 degrees this morning and a quick dipper of the culture, filtered thru a 37 micron and back washed into a petrie dish revealed several adult A. panamensis, very few rots, and 20 times as many star shaped nauplii. That was about 1/2 cup from a 17 gallon tub outdoors. Interstingly, last week that same tub had a dense culture of rotifers as well as panamensis. I guess the natural algae population could not keep up with the rotifer explosion. A. panamensis is sturdy, indeed. I may begin feeding this outdoor culture just to ensure that it continues. I may start an indoor culture from it, as my "clean" culture still struggles to proliferate.
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Re:Culture Journal, Species: Apocyclops panamensis
Wednesday, September 28, 2011 8:51 AM
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Here's a vid of a microscope view of the seived dipper ful of outdoor culture: Click on it! 
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Re:Culture Journal, Species: Apocyclops panamensis
Friday, September 30, 2011 5:38 AM
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i took a larger sampling of the outdoor culture, and it has a lot lot lot of M. salina adults in it. I'll have to check the nauplii appearance of the M. salina and the A. panamensis. I may have been mistaken, actually it is likely that I was.
My indoor culture has bloomed, and I can now start increasing the volume and feeding more O. marina. Yay!
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Re:Culture Journal, Species: Apocyclops panamensis
Sunday, October 16, 2011 3:31 PM
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I think there are still A panamensis in the outdoor culture, though there are no more rotifers in it, and there are definately some M. salina in there, splashed over, no doubt, from the neighboring tub.
The A. panamensis indoor "clean culture" are slowly increasing in population. I continue with a few drops of Andy's mix and chloramX twice a day, and about 100 ml per day of O. marina culture. Weekly decant to a clean gallon jar. The culture remains cloudy green. Sometimes I use RGcomplete instead of the Andy's mix and ChloramX.
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