Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Apocyclops panamensis Species description: egg carrying cyclopoid
Culture source (link if possible
): Kind gift of Jim Welsh, arrived 8/12/2011 via Fedex, room temp.
If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 8/12/2011
Continuation Date: 11/12/2011
Culturing Vessel Details Salinity: 20 ppt
Temperature: 75
pH: 8.2
Vessel description: One gallon square jar with wide mouth lid, hole in center, rigid airline connected to pump
Lighting description: 5500K fluorescent bulb,
Lighting cycle: 14 day/ 10 night
Aeration description: rapid, but not boiling
Methodologies Split methodology: Not sure yet, just trying to get a dense culture. Weekly, decant culture to clean jar.
Culture medium description: Food is 6 parts N-Rich, 1 part Roti-Grow+ (6NR/1RG+). 6 drops per feeding, 2 feedings per day. Equal volume of CloramX at each feeding.
Starting at week 2, 1/2 cup of O. marina culture added per day as well.
Cell count: Not known.
(if known)
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by KathyL on Monday, November 14, 2011 9:02 PM>