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Re:Culture Journal, Species: Apocyclops panamensis
Tuesday, October 18, 2011 6:11 PM
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Nearly crashed it. It turns out the O. marina had gone south, and I was adding ammonia water and ciliates to the culture of panamensis each day. There were enough to restart, however, and I have a saved jar of panamensis from my outdoor culture when it bloomed a couple of weeks ago, as a backup.
Gotta love the great outdoors. I've noticed that populations bloom in a big way, and then crash out there, making way for the next bloom of something else. It's pretty interesting and fun. Next year, I'll document better what I do and what the results are. This year was just playing around. I am surprised it was so prolific given that i've done next to nothing to keep it going.
I sieved the jar of panamensis through a 37 micron filter, and put the pods in some clean water with RGcomplete. This evening I fed a few drops of Andy's mix.
I've filtered the O. marina as well, keeping the flow through, and hopefully the ciliates contamination did not kill everything.
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Re:Culture Journal, Species: Apocyclops panamensis
Tuesday, November 1, 2011 6:06 PM
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The jar of panamensis recovered from the outdoor bloom are doing well with just a little food and no additional air. The indoor ones with daily food additions, just RotiGrow, NRich and ChloramX , and air and water additions are doing OK, though not as dense as they had previously been. I changed the jar today. Still concerned that my O marina crashed. I haven't been adding any O marina from my culture to the copepod cultures. I kept a jar of something that I got by filtering one of my other cultures (flow thru) and I think its O marina. I added rotifers as a size reference. What do you think? Are the dots O. marina or ciliates? 
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Re:Culture Journal, Species: Apocyclops panamensis
Wednesday, November 2, 2011 6:27 AM
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I have a couple of cultures of O marina. I'd be willing to give you one of them. well I guess more like give you one back lol.
RLTW 180 Gallon Mixed Reef Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8
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Re:Culture Journal, Species: Apocyclops panamensis
Wednesday, November 2, 2011 9:23 AM
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Just a sample would be great. Call me when you're going to be in town.
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Re:Culture Journal, Species: Apocyclops panamensis
Wednesday, November 2, 2011 9:51 AM
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will do.
RLTW 180 Gallon Mixed Reef Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8
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Re:Culture Journal, Species: Apocyclops panamensis
Sunday, November 13, 2011 7:48 AM
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Seems to be doing better lately, seems quite dense. I'll have to split today so they don't crash. Perhaps I'll start a 5 gallon bucket now.
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Re:Culture Journal, Species: Apocyclops panamensis
Monday, November 14, 2011 7:28 AM
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wrong again. they were not too dense, and a lot of rotifers in there. I filtered and changed to a clean jar and clean water.
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Re:Culture Journal, Species: Apocyclops panamensis
Tuesday, November 15, 2011 6:09 AM
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Re:Culture Journal, Species: Apocyclops panamensis
Monday, November 21, 2011 8:19 AM
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I kept the filtrate from one of my cultures, can't remember the filter size, darn it, and set it aside at room temperature. Now have a culture with lots of juvenile A. panamensis!!! I may be able to have a pure culture afterall. Let's hope there's no rotifers in it, as I have been keeping them near the rotifer buckets.
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Re:Culture Journal, Species: Apocyclops panamensis
Monday, November 21, 2011 8:38 AM
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I'd be worried about the Apocyclops contaminating my rotifers!  I find that some, but very few, Apocyclops nauplii will pass through an 80 micron sieve. Many, many of them will pas through 120 microns.
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Re:Culture Journal, Species: Apocyclops panamensis
Sunday, June 24, 2012 8:12 PM
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It's been a while since I logged in here. I've been ignoring my shelf cultures, but I have some panamensis in my S rotifer culture. I'm thinking its a good thing. The rotifer culture is cleaner and the panamensis get fed regularly. The panamensis are thriving, and there seems to be little deliterious effect on the rotifer population. I want panamensis to feed to my larvae, and all the larvae get rotifers, so why not culture them together? I wonder if this would work with parvocalanus and other desirable copepods.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Apocyclops panamensis
Monday, June 25, 2012 9:28 PM
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I've also got panamensis and S rotifers in a tub outdoors, and they are thriving. I first seeded the tub with some green micro algae, the remains of my isochrysis attempt, probably chlorella. After a couple of weeks of consistent green, I added a couple of gallons of the S rotifers mixed with some panamensis. Today, a couple of weeks later, I put 4 ounces thru a 53 micron filter and backwashed into a petrie dish. There was a good culture of rotifers, and hundreds of panamensis nauplii, and lots of egg bearing females. They are doing really well outdoors. High temps for the last week have been in the low 90s F. It's quite possible that the panamensis are eating the rotifers….
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Apocyclops panamensis
Tuesday, June 26, 2012 5:59 AM
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Eating the rotifers? That's interesting... Aren't the panamensis smaller than S rotifers?
Don't let fear and common sense stop you! =]
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Re:Culture Journal, Species: Apocyclops panamensis
Tuesday, June 26, 2012 6:59 AM
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I doubt the copepods are eating the rots. They are filter feeders and only eat really small stuff. It's quite possible to me that you've had a bloom of some small protist and the copepods are eating that.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Culture Journal, Species: Apocyclops panamensis
Tuesday, June 26, 2012 9:21 AM
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I'm with Andy on this one.
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Re:Culture Journal, Species: Apocyclops panamensis
Wednesday, April 17, 2013 12:23 AM
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After the heat of last summer, all my outdoor cultures crashed. And with enough neglect, I got some of the A panamensis away from the rotifers in my indoor culture. Now I have a great culture of A. panamensis, in gallon jars, fed only with live Iso. It's amazing. The cultures are pretty dense. I feed them very lightly with Iso, maybe 100 ml when they turn clear. Every week I change 100% of their water and give them new jars. I keep getting more and more cyclopods! I'll get pix in a short while.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Apocyclops panamensis
Wednesday, April 17, 2013 12:41 AM
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 Originally Posted by KathyL It's amazing. Isn't it, though?
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Re:Culture Journal, Species: Apocyclops panamensis
Wednesday, May 29, 2013 11:48 PM
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Freaky thing: Rotifers crashed just as a small nest of ocellaris hatched. I've started counting the A. panamensis I'm culturing as I almost lost them by trying to concentrate them too much. I had 6000 nauplii leftover after seeding my jars, so I gave them to the 6 gallon ocellaris larval tank along with what few rotifers I had. An hour later the larvae had noticable bulging tummies, and it's been like that for 3 days. I've made no further additions aside from the rotigreen I added on day one for green water. I'm going to get some more rotifers tomorrow, as I am afraid the remaining copepods will grow to be too large for the larvae before the larvae metamorphosize. There's only about 12 larvae in there, but they are not dying, and they look active and healthy.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Apocyclops panamensis
Wednesday, May 29, 2013 11:55 PM
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Nice!
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Re:Culture Journal, Species: Apocyclops panamensis
Sunday, June 9, 2013 1:21 PM
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Well, I was thinking that feeding with A panamensis might be better than rotifers, judging by the success of the small nest of ocellaris mentioned above. I had a larger nest hatch out, and I put them with the 4 day old smaller batch, and thought they were doing well. I added a lot more panamensis nauplii, but did not have the same response as the previous batch. In fact, many of them died over the 3 day period, as well as some of the previous dozen or so. The ones that survived look good. So this is not a tried an true method, and I still have some work to do.
This week, I have 3 nests hatching within a day of each other, and plan to put them all in the same tank, so I will be feeding them rotifers and panamensis nauplii and I'm really hoping that they do well. I kinda need these fish….
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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