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Culture Journal, Species: Tigriopus californicus
Saturday, August 20, 2011 8:32 PM
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Culturing Journal DataSheet This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Tigriopus californicus Species description: orange harpacticoid that is often free swimming. Culture source (link if possible ): Reed mariculture purchase If algae, CCMP # (Optional ): http://ccmp.bigelow.edu/ Culture Establishment Date: 11/16/2010 Continuation Date: 8/20/2011 and on from here. Culturing Vessel Details Salinity: 20 ppt Temperature: ambient room temp: ranges 62 in winter, to 78 in summer. pH: 7 to 8 ish Vessel description: 5 gallon bucket filled to 4 gallons Lighting description: ambient room compact fluorescents. Lighting cycle: 14 day, 10 night Aeration description: rapid but not boiling. Methodologies Split methodology: casual. Culture is thinned every few weeks, as well as partial water changes. Very low maintainance. Culture medium description: 20ppt water from water changes on broodstock systems. Food is mostly roti-grow+ with occational N-rich. Food is also occasional rotifer addition, as excess occurs. Cell count: I've never counted. Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: Can be kept for months in an open bottle in the fridge with periodic additions of food and chloramX. These pods do better in the cooler temperatures. Summer is harder on them but I had a nice outdoor culture until the weather got too hot. You will be required to provide photographic evidence and as much detail as possible about your project in this thread. If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
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Re:Culture Journal, Species: Tigriopus californicus
Sunday, August 21, 2011 11:45 AM
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Here's some pix: Here's the outdoor culture while it lived:
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Re:Culture Journal, Species: Tigriopus californicus
Sunday, August 21, 2011 12:05 PM
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Here's the indoor culture:
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Re:Culture Journal, Species: Tigriopus californicus
Sunday, August 21, 2011 12:07 PM
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Re:Culture Journal, Species: Tigriopus californicus
Sunday, August 21, 2011 12:11 PM
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Here is a video of the tiggers on a dish: there are other critters in there as well. I grow these as a mixed culture.
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Re:Culture Journal, Species: Tigriopus californicus
Sunday, August 21, 2011 12:25 PM
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Here's a bottle from the fridge:
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Re:Culture Journal, Species: Tigriopus californicus
Sunday, August 21, 2011 12:26 PM
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And the little twitchers that have lived in it for over a month, with small additions of food: CLICK ON THE VIDEO
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Re:Culture Journal, Species: Tigriopus californicus
Wednesday, September 28, 2011 7:17 AM
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i still have the bottles in the fridge. Recently topped them off with fresh water, and I'm feeding them a couple drops of rotigrow+ every 2 weeks or so. The poly-culture in the basement has some in there as well as the outdoor tub. Temperature at the water surface of the outdoor tube was 62, bottom outside of the tub was 64, and the patio concrete was 64. Temperature of the basement was 75. All temps in Farenheit.
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Re:Culture Journal, Species: Tigriopus californicus
Saturday, October 15, 2011 3:47 PM
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I had a tigger population explosion since the cool weather started here. Just gobs of them in the outdoor tub.
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Re:Culture Journal, Species: Tigriopus californicus
Saturday, October 29, 2011 3:00 PM
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Outdoor tubs seem to be all T. californicus now. They adhere to the sides of the tubs in a ring about 2 inches below the water surface, very densely. Then there are lots of them in the water column. Whereas M. salina was the major pod a week or so ago, the weather has gotten quite chilly here, and Tiggers are the only ones visible to the naked eye.
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Re:Culture Journal, Species: Tigriopus californicus
Saturday, October 29, 2011 3:02 PM
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Also, I still have these pods in the refrigerator. They continue living in open bottles with only periodic additions of food and chloramX. So if you are culturing these guys, put some in the fridge in case of a crash, and you will never run out!
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Re:Culture Journal, Species: Tigriopus californicus
Monday, November 14, 2011 6:35 PM
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i cleared out one of the tubs this past weekend. It was full of tigger pods. I got most of them as they were sitting on the sides of the tubs, not so much in the cold water. I left my filter in the bucket with the pods, and the next day the tiggers were grasping at the filter cloth and snuggled into every edge and crevice. I guess they really get benthic when cold.
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Re:Culture Journal, Species: Tigriopus californicus
Monday, November 14, 2011 7:26 PM
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I wish I would have read this when you got me that start. I lost them all. Hopefully I'll be able to start up another culture soon.
RLTW 180 Gallon Mixed Reef Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8
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Re:Culture Journal, Species: Tigriopus californicus
Thursday, November 17, 2011 5:37 PM
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Originally Posted by KathyL
i cleared out one of the tubs this past weekend. It was full of tigger pods. I got most of them as they were sitting on the sides of the tubs, not so much in the cold water. I left my filter in the bucket with the pods, and the next day the tiggers were grasping at the filter cloth and snuggled into every edge and crevice. I guess they really get benthic when cold. The get far less active below 60 F. but more so due to metabolism and less so to "being benthic". Their splash pools are very cold in winter and they do just fine. The only thing that really effects their wild population numbers is the tidal and storm activity. Our tides are greater on the west coast in the winter and when you add storms onto that, the splash pools can get a lot of action. In the summer the pools can go days to weeks seeing no wave action. Amazingly hardy creatures!!! Funny, the urban legend is they need to be cold to breed :lol: We've found to get the greatest culture density, it's best to keep them at reef tank temps!
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Re:Culture Journal, Species: Tigriopus californicus
Thursday, November 17, 2011 9:14 PM
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RGR, I still have gobs of them. Call me.
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Re:Culture Journal, Species: Tigriopus californicus
Friday, November 18, 2011 8:07 PM
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awesome will do! I'm going to try to head up that way soon. I may be able to talk the gf into doing it Sunday lol
RLTW 180 Gallon Mixed Reef Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8
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Re:Culture Journal, Species: Tigriopus californicus
Sunday, December 25, 2011 4:19 PM
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I finally cleared out the fridge cultures, since I have about 5 containers already that are full of T. cali. There were still some twitchers in there, alive for 6 months at 4C. Santa brought me a microscope, so there are some new pix: 40x. This one has eggs: Head portion Tail portion with eggs: RGRKing: did you get some new tigers? Ihave plenty to share.
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Re:Culture Journal, Species: Tigriopus californicus
Sunday, December 25, 2011 4:41 PM
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no, I'll hit you up next time I'm up if it's ok. Everything else is looking great! I can't believe they stayed alive for so long in the fridge. That's great!
RLTW 180 Gallon Mixed Reef Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8
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Re:Culture Journal, Species: Tigriopus californicus
Sunday, December 25, 2011 4:41 PM
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and what kind of microscope did you get?
RLTW 180 Gallon Mixed Reef Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8
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Re:Culture Journal, Species: Tigriopus californicus
Sunday, December 25, 2011 4:49 PM
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The ever popular Celestron!
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