Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Tigriopus californicus
Species description: orange harpacticoid that is often free swimming. Culture source (link if possible
): Reed mariculture purchase
If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 11/16/2010
Continuation Date: 8/20/2011 and on from here.
Culturing Vessel Details Salinity: 20 ppt
Temperature: ambient room temp: ranges 62 in winter, to 78 in summer.
pH: 7 to 8 ish
Vessel description: 5 gallon bucket filled to 4 gallons
Lighting description: ambient room compact fluorescents.
Lighting cycle: 14 day, 10 night
Aeration description: rapid but not boiling.
Methodologies Split methodology: casual. Culture is thinned every few weeks, as well as partial water changes. Very low maintainance.
Culture medium description: 20ppt water from water changes on broodstock systems. Food is mostly roti-grow+ with occational N-rich. Food is also occasional rotifer addition, as excess occurs.
Cell count: I've never counted.
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: Can be kept for months in an open bottle in the fridge with periodic additions of food and chloramX. These pods do better in the cooler temperatures. Summer is harder on them but I had a nice outdoor culture until the weather got too hot.
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