Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Nanochloripsis
Species description: Micro Algae Culture source (link if possible
): http://florida-aqua-farms.com If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 05/18/2011
Continuation Date: 09/07/2011
Culturing Vessel Details Salinity: 1.018
Temperature: Room Temperature at 70s
pH: not measured. Roughly 8.0
Vessel description: 2L soda bottle. Right after I finish drinking the soda, I rinse it a few times and soak it with tap water for days until whenever I'm about to use it. When I need it, I rinse it with tap water few more times then fill with RO water and salt. I do not sterilize them.
Lighting description: 4 tubes of Home Depot 3' T-5 light combo. The color looks like 3000K.
Lighting cycle: 18 hours a day
Aeration description: Very fast bubbling like boiling water.
Methodologies Split methodology: Pour out 1/4 of the bottle for feeding. Pour another 500ml into a new bottle for splitting to another culture.
When I split and create a new culture, I only use new bottles for the new culture. For the original dense culture bottle, after I pour out the amount that I use, I just refill it with salt water. The original bottle will be keep re-using until there are stuff or film developing near the bottle neck, then I just trash the bottle (by putting them into the city recycle bin. Not really trashing it). I do not clean or sterilize any them.
Culture medium description: 15-20 drops of F/2 into 2L of 1.018 aged salt water. The F/2 is the MICRO ALGAE GROW™ GUILLARD F/2 FORMULATION from Florida Aqua Farms.
Cell count: unknown
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by chuenwe on Wednesday, September 7, 2011 6:08 PM>