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•  Culture Journal, Species: Oxyrrhis marina

  Reports tied to this Journal Author Message Fishtal Total Posts : 5467 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Culture Journal, Species: Oxyrrhis marina Friday, September 16, 2011 1:56 PM (permalink) Culturing Journal DataSheet This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Oxyrrhis marina           Species description:  Dinoflagellate Culture source (link if possible):  Andrew Berry If algae, CCMP # (Optional):  http://ccmp.bigelow.edu/ Culture Establishment Date:  09-16-11 Continuation Date:  Culturing Vessel Details Salinity: 1.025           Temperature:  Ambient    pH:  not tested Vessel description: Starting in 1000ml  flask Lighting description:  Lighting cycle:  Aeration description:  Rigid airline, slow bubbles Methodologies Split methodology: Culture medium description:  (this could range, example with algae could be “10ml per l of guillards F formulation” or with A. tonsa it would be more like “300ml per day of algal mixture containing gymnodynium, tetraselmis, isochrysis, and rhodomonas” ) Cell count:  (if known) Reference links:   Additional Information (No Pictures or Videos in the Section Please) Notes:  Feeding Roti Grow Plus as needed. You will be required to provide photographic evidence and as much detail as possible about your project in this thread. If your thread does not contain detailed enough photos  and information the MBI Council will not be able to approve your reports. <message edited by Fishtal on Friday, September 16, 2011 2:27 PM> http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #1 Fishtal Total Posts : 5467 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Culture Journal, Species: Oxyrrhis marina Friday, September 16, 2011 2:05 PM (permalink) http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #2 KathyL Total Posts : 2639 Reward points : 1504 Joined: 6/6/2010 Location: St. Louis, MO, US Status: offline MBI: 1605 Pnts MBI Site: Marine Aquarium Societies of North America Re:Culture Journal, Species: Oxyrrhis marina Friday, September 16, 2011 3:30 PM (permalink) What's the magnification? If that was a rotifer in the first few frames, I think what you are seeing is ciliates.  O marina is a lot smaller.  Unless I'm wrong, which won't be the first time.   #3 Fishtal Total Posts : 5467 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Culture Journal, Species: Oxyrrhis marina Friday, September 16, 2011 4:07 PM (permalink) They didn't look like ciliates to me but I can check again. Once I get the cultures ramped up I'm going to experiment with 3 different containers to see if it makes a difference:   http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #4 KathyL Total Posts : 2639 Reward points : 1504 Joined: 6/6/2010 Location: St. Louis, MO, US Status: offline MBI: 1605 Pnts MBI Site: Marine Aquarium Societies of North America Re:Culture Journal, Species: Oxyrrhis marina Friday, September 16, 2011 7:48 PM (permalink) What was the magnification on that video?   #5 Umm_fish? Total Posts : 2835 Reward points : 953 Joined: 11/4/2009 Location: Boulder, CO, US Status: offline MBI: 775 Pnts MBI Site: Marine Aquarium Society of Colorado Re:Culture Journal, Species: Oxyrrhis marina Friday, September 16, 2011 8:08 PM (permalink) That's a ciliate in the first few frames, not a rotifer.   At least, I think so. --Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886   #6 Fishtal Total Posts : 5467 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Culture Journal, Species: Oxyrrhis marina Friday, September 16, 2011 8:12 PM (permalink) Originally Posted by KathyL What's the magnification? If that was a rotifer in the first few frames, I think what you are seeing is ciliates.  O marina is a lot smaller.  Unless I'm wrong, which won't be the first time. I "think" the magnification was 100X. Andy is correct, the big thing was a ciliate. I thought it was a rotifer at first too but I watched it again and looked closer. http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #7 KathyL Total Posts : 2639 Reward points : 1504 Joined: 6/6/2010 Location: St. Louis, MO, US Status: offline MBI: 1605 Pnts MBI Site: Marine Aquarium Societies of North America Re:Culture Journal, Species: Oxyrrhis marina Saturday, September 17, 2011 5:07 AM (permalink) That makes sense.  My microscope only goes to 40x, and OM look significantly smaller. At 100x they should be the size we see in your video. False alarm.   #8 JimWelsh Total Posts : 1426 Reward points : 1486 Joined: 1/22/2010 Location: Angwin, CA, US Status: offline MBI: 1845 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Oxyrrhis marina Saturday, September 17, 2011 9:22 AM (permalink) Definitely Oxyrrhis.  Shape and movement are correct.   #9 Fishtal Total Posts : 5467 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Culture Journal, Species: Oxyrrhis marina Friday, September 30, 2011 2:16 PM (permalink) How am I doing?       http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #10 JimWelsh Total Posts : 1426 Reward points : 1486 Joined: 1/22/2010 Location: Angwin, CA, US Status: offline MBI: 1845 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Oxyrrhis marina Friday, September 30, 2011 2:23 PM (permalink) Woot!  Nice and clean looking; no Euplotes visible.  I've seen denser, but that's pretty good.   #11 Fishtal Total Posts : 5467 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Culture Journal, Species: Oxyrrhis marina Friday, September 30, 2011 2:30 PM (permalink) Thanks, I just took a tiny sample off the top. http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #12 Umm_fish? Total Posts : 2835 Reward points : 953 Joined: 11/4/2009 Location: Boulder, CO, US Status: offline MBI: 775 Pnts MBI Site: Marine Aquarium Society of Colorado Re:Culture Journal, Species: Oxyrrhis marina Friday, September 30, 2011 4:21 PM (permalink) I hardly ever see any ciliates in my cultures. Probably has to do with the whole being a bazillion miles from the coast. --Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886   #13 Fishtal Total Posts : 5467 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Culture Journal, Species: Oxyrrhis marina Sunday, October 2, 2011 6:16 PM (permalink) Ok, I got a better sample... http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #14 KathyL Total Posts : 2639 Reward points : 1504 Joined: 6/6/2010 Location: St. Louis, MO, US Status: offline MBI: 1605 Pnts MBI Site: Marine Aquarium Societies of North America Re:Culture Journal, Species: Oxyrrhis marina Sunday, October 2, 2011 9:46 PM (permalink) I'm also a bazillion miles from the coast, but I get occasional ciliates.  This time, I think, from Jim W., not that I'm complaining.  He's sent much appreciated cultures, and the ciliates are a bonus, in my opinion.    Sometimes I get them from rotifers at the university.   #15 Fishtal Total Posts : 5467 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Culture Journal, Species: Oxyrrhis marina Thursday, October 20, 2011 6:53 PM (permalink) I scrapped the flask and am using the 2 different clear containers and they seem to be working fine.   I've been feeding RGC every day, or so, and the cultures are thriving. I strain each container every 3-4 days with a 53m sieve to get the yucky stuff out. http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #16 Online Bookmarks Sharing: Facebook Digg Google del.icio.us StumbleUpon

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