Culture Journal, Species: Oxyrrhis marina

General
Species: Oxyrrhis marina          
Species description:  Dinoflagellate
Culture source (link if possible):  Andrew Berry
If algae, CCMP # (Optional): 
http://ccmp.bigelow.edu/
Culture Establishment Date:  09-16-11
Continuation Date: 

Culturing Vessel Details
Salinity: 1.025          
Temperature:  Ambient   
pH:  not tested

Vessel description: Starting in 1000ml  flask
Lighting description: 
Lighting cycle: 
Aeration description:  Rigid airline, slow bubbles

Methodologies
Split methodology:

Culture medium description: 
(this could range, example with algae could be “10ml per l of guillards F formulation” or with A. tonsa it would be more like “300ml per day of algal mixture containing gymnodynium, tetraselmis, isochrysis, and rhodomonas” )

Cell count:
 (if known)

Reference links:  

Additional Information
(No Pictures or Videos in the Section Please)
Notes: 
Feeding Roti Grow Plus as needed.


You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos  and information the MBI Council will not be able to approve your reports.

http://www.fishtalpropagations.com/#!home/mainPage
"Making captive breeding easier."

http://www.fishtalpropagations.com/#!home/mainPage
"Making captive breeding easier."

What's the magnification? If that was a rotifer in the first few frames, I think what you are seeing is ciliates.  O marina is a lot smaller.  Unless I'm wrong, which won't be the first time.

They didn't look like ciliates to me but I can check again.

Once I get the cultures ramped up I'm going to experiment with 3 different containers to see if it makes a difference:
 

http://www.fishtalpropagations.com/#!home/mainPage
"Making captive breeding easier."

What was the magnification on that video?

That's a ciliate in the first few frames, not a rotifer.
 
At least, I think so.

--Andy, the bucket man.
"Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886

I "think" the magnification was 100X. Andy is correct, the big thing was a ciliate. I thought it was a rotifer at first too but I watched it again and looked closer.

http://www.fishtalpropagations.com/#!home/mainPage
"Making captive breeding easier."

That makes sense.  My microscope only goes to 40x, and OM look significantly smaller. At 100x they should be the size we see in your video. False alarm.

Definitely Oxyrrhis.  Shape and movement are correct.

How am I doing?
 



 
 

http://www.fishtalpropagations.com/#!home/mainPage
"Making captive breeding easier."

Woot!  Nice and clean looking; no Euplotes visible.  I've seen denser, but that's pretty good.

Thanks, I just took a tiny sample off the top.

http://www.fishtalpropagations.com/#!home/mainPage
"Making captive breeding easier."

I hardly ever see any ciliates in my cultures. Probably has to do with the whole being a bazillion miles from the coast.

--Andy, the bucket man.
"Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886

Ok, I got a better sample...

http://www.fishtalpropagations.com/#!home/mainPage
"Making captive breeding easier."

I'm also a bazillion miles from the coast, but I get occasional ciliates.  This time, I think, from Jim W., not that I'm complaining.  He's sent much appreciated cultures, and the ciliates are a bonus, in my opinion.
 
 Sometimes I get them from rotifers at the university.

I scrapped the flask and am using the 2 different clear containers and they seem to be working fine.
 
I've been feeding RGC every day, or so, and the cultures are thriving. I strain each container every 3-4 days with a 53m sieve to get the yucky stuff out.

http://www.fishtalpropagations.com/#!home/mainPage
"Making captive breeding easier."