Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Brachionus plicatilis
Species description: Rotifer - L strain
Culture source (link if possible
): Mike Taylor, CORA Frag Swap - his original was from livebrineshrimp.com
If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 9/18/11 Continuation Date: 12/18/11 (is this the date you had the culture for 3 months?) Culturing Vessel Details Salinity: 1.015
Temperature: 74
pH: 8.3
Vessel description: 5 gallon tank initially, moved to 5 gallon buckets
Lighting description: Ambient fluorescent shop lights
Lighting cycle: 8 hours / day
Aeration description: airstone initially, moved to airline tubing in one, airstone in the other bucket
Methodologies Split methodology: Removed 64 oz. of culture on 12/6 into new bucket, added 1.015 saltwater to original culture to bring volume back up and then doubled water volume of 2nd culture daily until up to ~4 gallons
Culture medium description: Saltwater
Cell count: ~50/ml
Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: Following Tal's guidelines for start-up of a rotifer culture, doubling water volume every day. Feeding right now with PhytoFeast until I purchase RotiGrow.
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If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by AmandaCox on Friday, December 23, 2011 7:34 AM>