Culturing Journal DataSheet
General Species: Brachionus plicatilis
Species description: L type rotifer
Culture source: Reed's Mariculture
www.reed-store.com Culture Establishment Date: Sept 2, 2011 (died out at Christmas due to neglect). Fresh culture started April 5, 2012.
Continuation Date: July 5, 2012.
Culturing Vessel Details Salinity: 1.014
Temperature: Ambient room temp of about 74-76F
pH: Not tested
Vessel description: 1 gallon fish bowls to start. Then (2) 5 gallon salt pails with lids set on top to help with evaporation. Currently using 3.5L grey buckets with clear lids.
Lighting description: Ambient natural and ambient room fluorescent
Lighting cycle: 14 hours light, 10 hours dark
Aeration description: Rigid airline open end. Gentle, mild bubbling.
Methodologies Split methodology: Day 1 - decant top 1/6 clean culture, dump rest of culture, gently clean bucket (no disinfectants), return 1/6 clean culture, add fresh saltwater to make 1/3 bucket volume. Day 2 - add 1/3 bucket volume to make 2/3 total volume. Day 3 - same as Day 2 to make 3/3 total volume. Day 4 - repeat starting at "Day 1".
Culture medium description: Rotifers fed about 2.25 mL RotiGrow Plus per liter of culture water split into 2 daily feedings. Water is still barely green at the next feeding. A ClorAm-X solution is made using 4 tsp ClorAm-X and enough water to make 250 mL of solution. The ClorAm-X solution is used in equal amounts of RG+ at each feeding.
Cell count: Not counted.
Reference links: Additional Information Notes:
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