Culturing Journal DataSheet
General Species: Artemia
Species description: Brine shrimp
Culture source (link if possible
): Brine Shrimp Direct, Grade A cysts.
www.brineshrimpdirect.com Culture Establishment Date: Aug 22, 2011
Continuation Date: Not continuous. Artemia hatched daily.
Culturing Vessel Details Salinity: 1.023
Temperature: 70F (ambient)
pH: Unknown
Vessel description: San Francisco Bay pop bottle hatcheries
Lighting description: Ambient, room fluorescent
Lighting cycle: 6:30 am to 11:30 pm
Aeration description: Airline hooked to bottom of hatchery, rough tumble.
Methodologies Split methodology: Not applicable
Culture medium description: Artemia over 6 hrs are enriched with N-Rich PL Plus for 2 hours.
Cell count: Unknown, add 1/8 tsp to each hatchery daily.
Reference links: Additional Information Cysts are decapsulated before hatching. I put the eggs into the hatchery with saltwater (1.018), running air for one hour to hydrate the eggs. Sieve the eggs out, then put the eggs back in with about a cup of full strength bleach. Let them bubble until they turn orange (about 5 mins). Sieve the eggs out, and rinse really well with tap water. They will still have a faint smell of bleach, but this doesn't seem to effect anything. Decapped eggs are stored in the fridge in extra high salinity water. I put about 2 tbsp of marine salt into about 100 mL of water over 2 tbsp of decapped eggs. This makes for no shells in the hatcheries.
Hatcheries are cleaned and dried daily with vinegar solution after BS are harvested.
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