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•  Culture Journal, Species: Parvocalanus crassirostris

  Reports tied to this Journal Author Message JimWelsh Total Posts : 1426 Reward points : 1486 Joined: 1/22/2010 Location: Angwin, CA, US Status: offline MBI: 1845 Pnts MBI Site: Marine Breeding Initiative (MBI) Culture Journal, Species: Parvocalanus crassirostris Tuesday, September 27, 2011 2:41 AM (permalink) Culturing Journal DataSheet This first post should be updated regularly to include new information as events take place or changes are made to your system General Species:  Parvocalanus crassirostris Species description:  Calanoid copepod with very small nauplii Culture source (link if possible):  Reed Mariculture If algae, CCMP # (Optional):  N/A http://ccmp.bigelow.edu/ Culture Establishment Date:  9/21/2011 Continuation Date:  Culturing Vessel Details Salinity:  1.026 (35 PPT) Temperature:    Low 70s F pH:  Not measured Vessel description:  1000 ml Erlenmeyer flasks Lighting description:  In my phytoplankton culture station Lighting cycle:  16 H on / 8 H off Aeration description:  Very gentle air with a rigid ariline. Methodologies Split methodology: Culture medium description:  Artificial salt water Cell count:   Unknowown  (if known) Reference links:   Additional Information (No Pictures or Videos in the Section Please) Notes:  You will be required to provide photographic evidence and as much detail as possible about your project in this thread. If your thread does not contain detailed enough photos  and information the MBI Council will not be able to approve your reports.   #1 JimWelsh Total Posts : 1426 Reward points : 1486 Joined: 1/22/2010 Location: Angwin, CA, US Status: offline MBI: 1845 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Parvocalanus crassirostris Tuesday, September 27, 2011 2:45 AM (permalink) I've only had these little guys for 5 days now, but they are multiplying like nobody's business!  I'm keeping them fed very lightly, but very consistently, with live Isochrysis and live Thalassiosira combined.  The cultures are getting very dense with all life stages.  I have also noticed many, many small, white, spherical eggs being deposited.  More on these copepods as I work with them, but my initial response is: "WOW!"   #2 JimWelsh Total Posts : 1426 Reward points : 1486 Joined: 1/22/2010 Location: Angwin, CA, US Status: offline MBI: 1845 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Parvocalanus crassirostris Tuesday, September 27, 2011 11:42 PM (permalink) I am a very happy camper.  Dense dense dense cultures of Parvo in both 1000 ml flasks I'm running, so I split them into 3 and am feeding more heavily now.   #3 JimWelsh Total Posts : 1426 Reward points : 1486 Joined: 1/22/2010 Location: Angwin, CA, US Status: offline MBI: 1845 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Parvocalanus crassirostris Thursday, September 29, 2011 2:10 AM (permalink) OK, I know I'm sounding redundant and boring, but the density of these copepods is really impressive!  It is interesting watching them, though, compared to other copepod species I've worked with.  They seem to just float motionlessly, for the most part, and then will suddenly take off swimming.  If it turn the air off for a couple of minutes, leave them in the dark, and let the detritus settle out, then observe them with a flashlight behind their container and a magnifying glass, I see a really dense culture.  They are strongly phtottrophic, but not all at once.  Many will just sit motionless for many seconds, and all the while various individuals will take off swimming suddenly towards the light.   Measuring them like I do for rotifers, I get something like 20-30 copepods of various sizes per ml.   #4 Fishtal Total Posts : 5467 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Culture Journal, Species: Parvocalanus crassirostris Wednesday, November 2, 2011 12:51 PM (permalink) Any updates? I just got new starters to play with. Have you tried feeding them RGC? http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #5 JimWelsh Total Posts : 1426 Reward points : 1486 Joined: 1/22/2010 Location: Angwin, CA, US Status: offline MBI: 1845 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Parvocalanus crassirostris Wednesday, November 2, 2011 7:11 PM (permalink) Not yet, Tal but I'm planning on doing so with this new set.  My earlier cultures got contaminated by Apocyclops (stupid, stupid, stupid me!).  It took a while, but the Apo did finally win out.   Now that I have fresh, clear Parvo cultures, I'll do some RGC and also Shellfish Diet tests with them, as soon as I get my stock cultures re-established.  Should be just a few days before I can start.   FWIW, I have had great success raising the Bluestripe pipefish fry with Parvocalanus alone for the first two weeks.  Anecdotally, the survival rates of the pipes seems to be better with Parvo alone than with Apocyclops or even a mixture.  I'm going to try to repeat that success soon.   #6 Fishtal Total Posts : 5467 Reward points : 2908 Joined: 8/31/2006 Location: Waterford, MI, US Status: offline MBI: 585 Pnts MBI Site: MarineLife Aquarium Society of Michigan Re:Culture Journal, Species: Parvocalanus crassirostris Wednesday, November 2, 2011 9:09 PM (permalink) All I have is RGC so I'm trying it. http://www.fishtalpropagations.com/#!home/mainPage "Making captive breeding easier."   #7 Umm_fish? Total Posts : 2835 Reward points : 953 Joined: 11/4/2009 Location: Boulder, CO, US Status: offline MBI: 775 Pnts MBI Site: Marine Aquarium Society of Colorado Re:Culture Journal, Species: Parvocalanus crassirostris Wednesday, November 2, 2011 11:10 PM (permalink) I've managed to keep them alive on O. marina, but not thrive like you are talking about, Jim. Good for you! Mine seem just as lethargic in the water column, though. I'm trying another bunch with a little different protocol to see if I can do any better. --Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886   #8 luis a m Total Posts : 451 Reward points : 1249 Joined: 3/15/2011 Location: Buenos Aires, Buenos Aires, AR Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Parvocalanus crassirostris Wednesday, November 2, 2011 11:47 PM (permalink) My cultures are also doing very well,dense and lively,supporting partial harvest every other day.Naups like to swim showing their comma like profile,good diagnostic feature I´m glad I can post here,most copepod threads are closed for me. BTW Jim,your avatar shows a nauplius molt,not a copepodite´s   #9 JimWelsh Total Posts : 1426 Reward points : 1486 Joined: 1/22/2010 Location: Angwin, CA, US Status: offline MBI: 1845 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Parvocalanus crassirostris Thursday, November 3, 2011 12:15 AM (permalink) Originally Posted by luis a m I´m glad I can post here,most copepod threads are closed for me.   Hunh?  Are you having trouble posting in other threads on MBI? Originally Posted by luis a m BTW Jim,your avatar shows a nauplius molt,not a copepodite´s   I stand corrected!  Thanks, Luis!   #10 aomont Total Posts : 774 Reward points : 1003 Joined: 2/12/2011 Location: Rio de Janeiro, RJ, BR Status: offline MBI: 5 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Parvocalanus crassirostris Thursday, November 3, 2011 6:32 AM (permalink) Originally Posted by JimWelsh Originally Posted by luis a m I´m glad I can post here,most copepod threads are closed for me. Hunh?  Are you having trouble posting in other threads on MBI? The threads in "Intro to Culturing Pelagic Copepods" are closed to "non-members" so if you're not in you can't post. Anderson.   #11 KathyL Total Posts : 2639 Reward points : 1504 Joined: 6/6/2010 Location: St. Louis, MO, US Status: offline MBI: 1605 Pnts MBI Site: Marine Aquarium Societies of North America Re:Culture Journal, Species: Parvocalanus crassirostris Thursday, November 3, 2011 9:26 AM (permalink) I think we should let Luis in as a guest professor. JMO I am finally having some success with Isochrysis, so I'll be ordering some P. crassirostris soon.  Does anyone know the origin of the name? Rostris usually means nose, but I doubt these critters have one.   #12 luis a m Total Posts : 451 Reward points : 1249 Joined: 3/15/2011 Location: Buenos Aires, Buenos Aires, AR Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Parvocalanus crassirostris Thursday, November 3, 2011 10:36 AM (permalink) Nice gag,Kathy! Crassus=fat,thick. Rostrum=face     Originally Posted by KathyL I think we should let Luis in as a guest professor. JMO I am finally having some success with Isochrysis, so I'll be ordering some P. crassirostris soon.  Does anyone know the origin of the name? Rostris usually means nose, but I doubt these critters have one.   #13 Umm_fish? Total Posts : 2835 Reward points : 953 Joined: 11/4/2009 Location: Boulder, CO, US Status: offline MBI: 775 Pnts MBI Site: Marine Aquarium Society of Colorado Re:Culture Journal, Species: Parvocalanus crassirostris Thursday, November 3, 2011 11:44 AM (permalink) The class size limit was not really meant as a way to limit discussion, just as a protection for me so I didn't spend the entire fall organizing and shipping so many free cultures that I didn't have time to do the things I do to actually make money. I can put you in there if you'd like, Luis. --Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886   #14 luis a m Total Posts : 451 Reward points : 1249 Joined: 3/15/2011 Location: Buenos Aires, Buenos Aires, AR Status: offline MBI: 0 Pnts MBI Site: Marine Breeding Initiative (MBI) Re:Culture Journal, Species: Parvocalanus crassirostris Thursday, November 3, 2011 12:15 PM (permalink) I understand this and in no way was I complaining.Just sometimes I could add my experiences on the subject,if this could help   #15 Umm_fish? Total Posts : 2835 Reward points : 953 Joined: 11/4/2009 Location: Boulder, CO, US Status: offline MBI: 775 Pnts MBI Site: Marine Aquarium Society of Colorado Re:Culture Journal, Species: Parvocalanus crassirostris Thursday, November 3, 2011 12:28 PM (permalink) Done. --Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886   #16 Online Bookmarks Sharing: Facebook Digg Google del.icio.us StumbleUpon

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