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So, some of you should have cultures. How's it going?
Wednesday, September 28, 2011 6:38 PM
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Experiences. Questions. Comments. How are things going?
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:So, some of you should have cultures. How's it going?
Wednesday, September 28, 2011 6:59 PM
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So far, so good. I have several cultures of each going but haven't checked for density numbers yet.
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Re:So, some of you should have cultures. How's it going?
Wednesday, September 28, 2011 7:02 PM
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My O.marina is doing great. (I think... I ordered a scope TODAY so I'll know soon what I have.) My pods started off in a culture of too much water (my fault) and they are off to a really slow start. I think they are starting to come around but only time will tell.
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Re:So, some of you should have cultures. How's it going?
Thursday, September 29, 2011 7:15 AM
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Good, good. I'm glad hear it. (Mua-ha-ha. The evil laugh just seems appropriate for some reason.) Adam, they should ramp up really quickly. I've gone from maybe one or two individuals to them completely taking over a parvocalanus culture in a month. Don't give up on them.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:So, some of you should have cultures. How's it going?
Thursday, September 29, 2011 3:13 PM
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My O.marina is hard to tell what's going. I need to reread the thread to identify what is it I'm looking for. Pods are still alive. What is the reason I can't feed then Rotigrow or Rotigreen?
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Re:So, some of you should have cultures. How's it going?
Thursday, September 29, 2011 3:20 PM
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They are really small, so you really need a back light to see them. Is your culture taking on a brown color, even when you are just about to feed again? Pods: You can feed them anything you want to. They just may not survive on it. (See here: http://www.mbisite.org/Forums/tm.aspx?m=60961&high=.) I'm just giving you the method that works for me. Whether you follow it is up to you. Cultures fed pastes tend to ultimately be very unstable cultures. If you do that, I would keep a _lot_ of extra cultures running.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:So, some of you should have cultures. How's it going?
Thursday, September 29, 2011 3:54 PM
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 Originally Posted by Umm_fish?
Good, good. I'm glad hear it. (Mua-ha-ha. The evil laugh just seems appropriate for some reason.) Adam, they should ramp up really quickly. I've gone from maybe one or two individuals to them completely taking over a parvocalanus culture in a month. Don't give up on them. I see a bunch of little guys swimming around and then every time I check (daily) I see more and more adults. I dont have a scope yet (comes in a couple of days) to see how many gravid females I have but even if I did, I wouldn't disturb the culture right now. I'll keep you updated.
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Re:So, some of you should have cultures. How's it going?
Monday, October 3, 2011 1:47 PM
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Everyday I look twice and it seems there are more and more large adults along with more little guys. I would say I started with ~20-40 adults and I think I have well over 100 now. I get my scope today in the mail so I'll be taking some shots if I have some free time.
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Dinos!
Thursday, October 6, 2011 6:35 PM
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Re:Dinos!
Thursday, October 6, 2011 7:12 PM
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Good looking culture, Adam!
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Re:Dinos!
Thursday, October 6, 2011 7:21 PM
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Yay! You got your 'scope working!
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Copepod molt??
Thursday, October 6, 2011 7:37 PM
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Re:Copepod molt??
Thursday, October 6, 2011 7:41 PM
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Molt. That's also what my avatar image is, a molt of an Apocyclops copepodite.
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Re:Copepod molt??
Friday, October 7, 2011 9:04 AM
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Yes, very nice! Congratulations on what certainly look like some healthy dino cultures.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Copepod molt??
Tuesday, October 18, 2011 11:26 PM
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My cultures were taking a dive as it is getting cold in my basement. I just fixed that with a tub of water and a heater with my cultures in that and then it is covered. Hoping it will get my through the winter. Ciliate?
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Re:Copepod molt??
Wednesday, October 19, 2011 8:07 AM
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That looks a lot like O. marina to me. Without seeing it move it's hard to tell for sure. But I'd say flagellate.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Copepod molt??
Wednesday, October 19, 2011 8:28 AM
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Re:Copepod molt??
Wednesday, October 19, 2011 8:35 AM
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Thanks. Seeing the movement and size differential makes it much easier. Yeah, that's a ciliate, and quite a large one. Maybe Euplotes? I'm not very good at protist ID.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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Re:Copepod molt??
Wednesday, October 19, 2011 9:17 AM
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I don't know what it is, but it has neither the right shape nor the right movement for what I understand Euplotes to look like.
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Re:Copepod molt??
Wednesday, October 19, 2011 10:18 AM
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Well, you have a lot more experience with Euplotes than I do, Jim.  Yeah, whatever it is can be easily filtered out of the culture. But maybe let it multiply a bit first, then filter it and wash it into its own culture and see what you come up with? You might have the next big thing there.
--Andy, the bucket man. "Not to know the mandolin is to argue oneself unknown...." --Clara Lanza, 1886
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