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Culture Journal, Species: Tigriopus californicus
Friday, September 30, 2011 11:57 PM
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Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Tigriopus californicus Species description: Harpacticoid copepod Culture source (link if possible ): Reef Nutrition http://wiki.reefnutrition.com/w/page/14004561/Tigger-Pods%20FAQ If algae, CCMP # (Optional ): http://ccmp.bigelow.edu/ Culture Establishment Date: 9/30/2011, sometime in 2012 that also failed prior to continuation date and now this attempt started on 1/31/13 Continuation Date: ~12/30/2011 (old), new continuation date 4/30/13. I am now at twice the continuation date and still producing and harvesting 100s of T. californicus pods each day from each 5 gallon bucket. Culturing Vessel Details Salinity: 1.020 Temperature: Ambient room temperature 68 - 72 F pH: 8.3
Vessel description: 5 gallon pail (filled with approximately 4 gallons) Lighting description: ambient room light from window at a distance Lighting cycle: based upon natural photoperiod Aeration description: One large airstone at a slow rate of flow Methodologies Split methodology: harvest as needed. Culture medium description: Rotigrow plus sufficient to lightly tint the water green and supplimented with finely ground flake food. Cell count: Started with two bottles. Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
<message edited by reeflover on Thursday, August 15, 2013 2:13 AM>
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Re:Culture Journal, Species: Tigriopus californicus
Tuesday, October 11, 2011 3:57 PM
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Started to get an increase in ammonia noted last week, so a pre-seasoned piece of bioribbon from a CPR bak-pak 2 protein skimmer was added to the pail along with one piece of live rock to increase surface area for the pods and to provide some biological filtration.
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Re:Culture Journal, Species: Tigriopus californicus
Friday, December 9, 2011 4:40 PM
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Re:Culture Journal, Species: Tigriopus californicus
Friday, December 9, 2011 4:42 PM
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Re:Culture Journal, Species: Tigriopus californicus
Friday, December 9, 2011 4:49 PM
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I'd increase your salinity. They rarely see even normal salinity in the wild. Consider them like artemia in this sense.
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Re:Culture Journal, Species: Tigriopus californicus
Friday, December 9, 2011 5:08 PM
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 Originally Posted by GreshamH
I'd increase your salinity. They rarely see even normal salinity in the wild. Consider them like artemia in this sense.
Guess I'm not sure what you mean. Are you saying they would prefer hypersalinity? Artemia usually do best under lower salinity than seawater, which is what I am doing with the pods.
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Re:Culture Journal, Species: Tigriopus californicus
Friday, December 9, 2011 5:28 PM
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I've always done them at 1.025 - 1.030. Our supplier, Artemia International suggests 1.025 - 1.030. LIke anything, source of cysts has to do with how they live... GSL, SFB, Russia, China, etc.
Tigger-Pods do best at or above "normal" salinity. If you want to grow them for use, do that. If you just want to play with them, don't worry about it. Your temps are on the cool side as well so couple with the low salinity, your production will be suppressed.
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Re:Culture Journal, Species: Tigriopus californicus
Saturday, March 16, 2013 1:48 AM
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I've done several trials now with these pods, and have never managed to get a good "crop" of them since my first time when the culture became contaminated with my microalgae culture. Last month (2/1/13) I restarted my culture in plastic shoe boxes fed phytofeast. I put these cultures in the sun so that I get some water evaporation and higher day time temps and lower evening temps. There has been a slow progression of the cultures but I wouldn't call is explosive by any means. Then someone on MBI told me about co-culturing these pods with rotifers. Since I have been having so much luck with my rotifer cultures, I thought I would give it a shot. I can't believe how well the pods are doing in with the rotifers. I have since tried adding a few rotifers to the shoe box cultures to see if that would make a difference, but still nothing impressive. From my experience, polyculture with rotifers is the easiest and best method to grow any kind of numbers of T. californicus indoors.
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Re:Culture Journal, Species: Tigriopus californicus
Saturday, March 16, 2013 1:54 AM
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Originally Posted by GreshamH
I've always done them at 1.025 - 1.030. Our supplier, Artemia International suggests 1.025 - 1.030. LIke anything, source of cysts has to do with how they live... GSL, SFB, Russia, China, etc.
Tigger-Pods do best at or above "normal" salinity. If you want to grow them for use, do that. If you just want to play with them, don't worry about it. Your temps are on the cool side as well so couple with the low salinity, your production will be suppressed.
Certainly Reed Mariculture has the answer to these two questions: 1) What is the ideal salinity for culturing Tigriopus californicus? 2) What is the ideal temperature for culturing Tigriopus californicus? Perhaps these two questions are interrelated, in which case feel free to interrelate the answer! 
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Re:Culture Journal, Species: Tigriopus californicus
Saturday, March 16, 2013 11:40 AM
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I know what you are saying Jim, but I am trying to find an easy method for culturing. With the shoe boxes in the window I am trying to re-create the CA environment with warm days and cool evenings, and I am allowing these to slowing increase in salinity through evaporation. Each box gets bubbled for 24 hours every 72 hours. The little shoe boxes are like mini greenhouses when the sun hits them and the water really gets warm fast, but cools down again over night. However, the rotifer buckets are room time (cool) and hyposalinic (~1.015) yet the T. californicus seem to be thriving under those conditions. So I am beginning to think that it is not the salinity and temperature alone that is necessary for productive cultures. Does that make sense?
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Re:Culture Journal, Species: Tigriopus californicus
Tuesday, March 19, 2013 5:40 AM
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Originally Posted by JimWelsh
Originally Posted by GreshamH
I've always done them at 1.025 - 1.030. Our supplier, Artemia International suggests 1.025 - 1.030. LIke anything, source of cysts has to do with how they live... GSL, SFB, Russia, China, etc.
Tigger-Pods do best at or above "normal" salinity. If you want to grow them for use, do that. If you just want to play with them, don't worry about it. Your temps are on the cool side as well so couple with the low salinity, your production will be suppressed.
Certainly Reed Mariculture has the answer to these two questions: 1) What is the ideal salinity for culturing Tigriopus californicus? 2) What is the ideal temperature for culturing Tigriopus californicus? Perhaps these two questions are interrelated, in which case feel free to interrelate the answer! http://bit.ly/YPSlDt Besides what said, the website has a salinity range and temperature range. Other than what I have said here, and what the website says, I don't have much more to add. You're really asking the wrong person if you want greater detail. Attend http://www.mbiworkshop.com/ and maybe one of the speakers there will have more details 
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Re:Culture Journal, Species: Tigriopus californicus
Tuesday, March 19, 2013 5:52 AM
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Originally Posted by reeflover
I know what you are saying Jim, but I am trying to find an easy method for culturing. With the shoe boxes in the window I am trying to re-create the CA environment with warm days and cool evenings, and I am allowing these to slowing increase in salinity through evaporation. Each box gets bubbled for 24 hours every 72 hours. The little shoe boxes are like mini greenhouses when the sun hits them and the water really gets warm fast, but cools down again over night. However, the rotifer buckets are room time (cool) and hyposalinic (~1.015) yet the T. californicus seem to be thriving under those conditions. So I am beginning to think that it is not the salinity and temperature alone that is necessary for productive cultures. Does that make sense?
Feed and DO are important as well.
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Re:Culture Journal, Species: Tigriopus californicus
Monday, April 8, 2013 8:10 PM
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I have to say I am VERY pleased with my T. californicus culture that being co-cultured with rotifers. The population has exploded and there are just tons of pods (well at least thousands). They are very easy to separate from the rotifers when I need them for feeding to fish fry.
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Re:Culture Journal, Species: Tigriopus californicus
Monday, April 8, 2013 8:32 PM
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I'm getting a starting culture of these soon as well. Why does co-culturing them work like that?
Should I be co-culturing my other pod colonies?
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Re:Culture Journal, Species: Tigriopus californicus
Monday, April 8, 2013 11:21 PM
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Not sure why it works so well, but it sure seems to. Also don't know if it would work with other pods or not. Maybe you could try and let us know the results. Good luck!
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Re:Culture Journal, Species: Tigriopus californicus
Monday, April 8, 2013 11:36 PM
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I've noticed a similar success with Tigriopus co-cultured with rotifers. I'm pretty sure the Tigriopus are eating the rotifers.
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Re:Culture Journal, Species: Tigriopus californicus
Tuesday, April 9, 2013 2:51 AM
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Originally Posted by GreshamH http://bit.ly/YPSlDt Besides what said, the website has a salinity range and temperature range. Other than what I have said here, and what the website says, I don't have much more to add. You're really asking the wrong person if you want greater detail. Attend http://www.mbiworkshop.com/ and maybe one of the speakers there will have more details I'm confused about why you provided a bit.ly link to a lmgtfy.com link to a Google search for "www.tiggerpods.com", when simply providing a link to www.tiggerpods.com would have been much more straightforward, but whatever, dude. I found the temperature info on www.tiggerpods.com, where they have it in bold for emphasis, saying, " copepod populations were found at water temperatures of 6–33°C (42F to 92F)." Good to know -- thanks! I'll try to keep my Tigriopus between those two extremes. FWIW, 6˚C = 43˚F, and 33˚C = 91˚F (but who's counting?). Thanks also for the MBI Workshop suggestion. It really had never occurred to me that I might be able to fly 2,500 miles several months from now in order to get an answer to a question that a civil forum response could have provided.
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Re:Culture Journal, Species: Tigriopus californicus
Tuesday, April 9, 2013 9:33 AM
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Originally Posted by JimWelsh
I've noticed a similar success with Tigriopus co-cultured with rotifers. I'm pretty sure the Tigriopus are eating the rotifers.
That the Tigriopus pods are eating the rotifers are certainly one possibility. But I have also noticed that the rotifer cultures become more stable as well with less chance of a crash, but still with great numbers of rotifers, so I am wondering if the pods aren't just eating the bacteria (or over microorganisms like cilliates) or decomposing bodies of dead rotifers, thereby cleaning up the debris, and perhaps even the rotifer excriment, which I'm sure is small and nutrious.
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Re:Culture Journal, Species: Tigriopus californicus
Tuesday, April 9, 2013 1:20 PM
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How do you guys manage ammonia levels for these? Do I need to be testing for ammonia at all?
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Re:Culture Journal, Species: Tigriopus californicus
Tuesday, April 9, 2013 2:55 PM
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Originally Posted by nrbelk
How do you guys manage ammonia levels for these? Do I need to be testing for ammonia at all?
In my experience, the rotifers are more sensitive to ammonia than the pods are. If your water is good enough for the rotifers, the Tigriopus should do just fine. I don't test ammonia, but I add ClorAm-X with every RG+ feeding.
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