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Re:Culture Journal, Species: Tigriopus californicus
Tuesday, April 9, 2013 2:57 PM
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 Originally Posted by JimWelsh
Originally Posted by GreshamH http://bit.ly/YPSlDt Besides what said, the website has a salinity range and temperature range. Other than what I have said here, and what the website says, I don't have much more to add. You're really asking the wrong person if you want greater detail. Attend http://www.mbiworkshop.com/ and maybe one of the speakers there will have more details  I'm confused about why you provided a bit.ly link to a lmgtfy.com link to a Google search for " www.tiggerpods.com", when simply providing a link to www.tiggerpods.com would have been much more straightforward, but whatever, dude. I found the temperature info on www.tiggerpods.com, where they have it in bold for emphasis, saying, " copepod populations were found at water temperatures of 6–33°C (42F to 92F)." Good to know -- thanks! I'll try to keep my Tigriopus between those two extremes. FWIW, 6˚C = 43˚F, and 33˚C = 91˚F (but who's counting?). Thanks also for the MBI Workshop suggestion. It really had never occurred to me that I might be able to fly 2,500 miles several months from now in order to get an answer to a question that a civil forum response could have provided. Ouch Jim!
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Re:Culture Journal, Species: Tigriopus californicus
Thursday, April 11, 2013 9:52 PM
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Originally Posted by reeflover
Originally Posted by JimWelsh
I've noticed a similar success with Tigriopus co-cultured with rotifers. I'm pretty sure the Tigriopus are eating the rotifers.
That the Tigriopus pods are eating the rotifers are certainly one possibility. But I have also noticed that the rotifer cultures become more stable as well with less chance of a crash, but still with great numbers of rotifers, so I am wondering if the pods aren't just eating the bacteria (or over microorganisms like cilliates) or decomposing bodies of dead rotifers, thereby cleaning up the debris, and perhaps even the rotifer excriment, which I'm sure is small and nutrious. I'm with you on this. I've tried culturing them indoors on nothing but live Iso, and they went from bright orange to clear within 2 weeks. Many died. Might have been water quality, but I think they are carnivores or ichthevores (?fish eaters) and I'm pretty sure they are roti-vores. What I mean is I doubt that they are strictly vegetarians. Outside culture with rotifers is simply effortless.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Tigriopus californicus
Thursday, April 11, 2013 10:19 PM
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They certainly are not strict herbivores... but they'd be hard pressed to capture live rotifers. They have no fish to feed on in their natural environment, or smaller copepods. They do get a lot of dead and decaying crabs and seaweed.
Rotifers to us, in our cultures, are a bad thing. They eat all the phytoplankton and make for a worse copepod culture. Ours stay bright red and are fed solely on phytoplankton... and no sun on them, inside.
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Re:Culture Journal, Species: Tigriopus californicus
Friday, April 12, 2013 2:09 AM
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LOL, I was actually just repeating something that I read somewhere, but not cannot recall where (shame on me for that). What I can say is that I do notice a strange correlation in my Tigriopus cultures between the existence of rotifers, and an increase in the density of the Tigriopus in the cultures. I really don't know what the root cause of my anecdotal observation is, or if it will really hold out over time.
I generally have an extreme phobia of having rotifers contaminate my copepod cultures, and have recently implemented measures whereby I am pasteurizing all of my copepod culture water to avoid cross-contamination by either rotifers or different copepod species (and these measures seem to be working well), since I am currently culturing seven different species of copepods and rotifers and also Moina salina, all in the same room.
My Tigriopus cultures, on the other hand, are the exception to this rule. In fact, I fear that Tigriopus will contaminate my rotifer cultures, which has happened to me more than once. I find that Tigriopus will do very well in a rotifer culture, and then I end up with Tigriopus in my larvae tanks, which is not necessarily a good thing. Accordingly, I also take measures to avoid cross contamination of my rotifer cultures with Tigriopus.
Suffice it to say that in general, co-culturing (a very nice term for "cross contamination") is not a good thing, and should be avoided. It is always very easy to mix two cultures of things to get a co-culture. It is usually very, very difficult to separate a co-culture of any two organisms into clean cultures of each (or either).
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Re:Culture Journal, Species: Tigriopus californicus
Friday, April 12, 2013 8:49 AM
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Originally Posted by GreshamH
They certainly are not strict herbivores... but they'd be hard pressed to capture live rotifers. They have no fish to feed on in their natural environment, or smaller copepods. They do get a lot of dead and decaying crabs and seaweed.
Rotifers to us, in our cultures, are a bad thing. They eat all the phytoplankton and make for a worse copepod culture. Ours stay bright red and are fed solely on phytoplankton... and no sun on them, inside.
Which phytoplankton? I had perhaps added too much live Iso to my original tigriopus culture. The Iso began to get darker, and the tigers started dying. I have read that the fluid that comes off of frozen fish food when it thaws, added to the culture, can make it bloom with tigriopus. I've tried a ml of such fluid this evening. If it is just stress that makes the tigriopus go clear, then perhaps mine will recover. There are still some live ones in there. I am still feeding them live Isochrysis, just not so much.
check out Kathy's Clowns, llc website: http://kathysclowns.com Captive bred clownfish and more (Wholesale to the trade.)
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Re:Culture Journal, Species: Tigriopus californicus
Friday, April 12, 2013 12:13 PM
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Would tetra be a good phyto to feed these along with iso?
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Re:Culture Journal, Species: Tigriopus californicus
Saturday, June 22, 2013 12:20 PM
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Okay, hit continuation with this polyculture some time ago. It was started sometime in Jan 2013 when I restarted my rotifer cultures. Got so involved in raising my perc clowns, that I forgot to write down the exact date. I am now harvesting 100s of T. californicus pods from each 5 gallon bucket per night. They are being passed through a 180 micron screen that is nested into my 53 micron rotifer screen. I just pore the 6 liters of polyculture water through the 180 screen and what ever pods are in it are fed to the baby clowns or cardinals. Since it was started sometime in Jan 2013, I know I have gotten them well passed continuation, and still going. I can't believe how many pods these cultures produce for harvest every day. See pictures below.
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Re:Culture Journal, Species: Tigriopus californicus
Thursday, August 15, 2013 1:55 AM
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Okay, I just had to show off this latest batch. Last weekend I cleaned the bottoms of my 2 rotifer/T. californicus cultures and placed the waste water into an empty 5 gal bucket. There were so many pods I couldn't believe it. All of those little red dots in the bucket are T. californicus pods!!!!! I have been feeding them some rotifers and RG+ and in another 3 days will start feeding them to my newly hatch Gold Stripe Maroon larvae instead of NHBBS.
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