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Culture Journal, Species: Rhodomonas lens
Tuesday, October 11, 2011 12:55 AM
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Culturing Journal DataSheet This first post should be updated regularly to include new information as events take place or changes are made to your system General Species: Rhodomonas lens Species description: Red flagellated diatom Culture source (link if possible ): Algagen (via SeahorseSource.com) http://www.seahorsesource...p/detail.cgi?id=201133 If algae, CCMP # (Optional ): CCMP 739 http://ccmp.bigelow.edu/ Culture Establishment Date: 10/6/2011 Continuation Date: Culturing Vessel Details Salinity: 1.020 Temperature: Low 70s F pH: Not measured Vessel description: 2000 ml borosilicate glass media jars Lighting description: 2 x T5 21 watt 6500K fluorescent bulbs. Lighting cycle: 18 H on / 6 H off Aeration description: Moderate Methodologies Split methodology: To be determined. First split was at just 4 days, after a substantial number of dark red cells began to settle and needed constant swirling to keep them suspended. Culture medium description: 1200 ml of used tank water is mixed with 300 ml of RO/DI water, and placed into the media jar. 2 ml of a sodium metasilicate solution is added. A foam stopper is used to close the jar, and two such jars are microwaved for 25:80 (26 minutes, and 20 seconds; touch numbers 2,5,8, and 0 down the microwave keypad) in an 1100 watt microwave. Let cool to room temperature before using. After cooling, add 0.75 - 0.80 ml of FAF Micro Algae Grow immediately before adding 500 ml of a relatively dense culture as an innoculant. Cell count: Unknown Reference links: Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread. If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.
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Re:Culture Journal, Species: Rhodomonas lens
Tuesday, October 11, 2011 1:01 AM
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Re:Culture Journal, Species: Rhodomonas lens
Tuesday, October 11, 2011 1:57 PM
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mmmm pretty red rhodo god I love this species Jim, try mixing in a few 3400K bulbs with your 6500's I've had much better luck with Rhodomonas (salina and lens) with a mixed spectrum!
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Re:Culture Journal, Species: Rhodomonas lens
Friday, September 28, 2012 2:20 PM
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Any updates Jim?
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Re:Culture Journal, Species: Rhodomonas lens
Friday, September 28, 2012 7:36 PM
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Not really. I did run it for a while, but eventually it crashed on me. I should look for some images to see if I made it to my 90 Days Culture Continuation date! The A. tonsa did seem to like it, though.
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