Culture Journal, Species: Oxyrrhis Marina

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Caesra
  • Total Posts : 317
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  • Joined: 7/4/2011
  • Location: Cherry Valley, IL, US
Culture Journal, Species: Oxyrrhis Marina - Friday, October 14, 2011 10:13 PM
Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system

General
Species:  Oxyrrhis Marina
Species description:  Dinoflagellate 
Culture source (link if possible):  From Andy, Umm_fish
If algae, CCMP # (Optional): 
http://ccmp.bigelow.edu/
Culture Establishment Date:  10/13/2011
Continuation Date: 

Culturing Vessel Details
Salinity:  1.020
Temperature:  76 
pH:  not measured

Vessel description:  2.5g bucket - black
Lighting description:  compact florescent - ambient
Lighting cycle:  10/14
Aeration description: low bubble rate, roughly 2 per second 

Methodologies
Split methodology:

Culture medium description: 
(this could range, example with algae could be “10ml per l of guillards F formulation” or with A. tonsa it would be more like “300ml per day of algal mixture containing gymnodynium, tetraselmis, isochrysis, and rhodomonas” )

Cell count:
 (if known)

Reference links:  

Additional Information
(No Pictures or Videos in the Section Please)
Notes: 



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If your thread does not contain detailed enough photos  and information the MBI Council will not be able to approve your reports.


Caesra
  • Total Posts : 317
  • Reward points : 195
  • Joined: 7/4/2011
  • Location: Cherry Valley, IL, US
Re:Culture Journal, Species: Oxyrrhis Marina - Friday, February 3, 2012 8:11 PM
Well, so far these are still going along just fine.  Really has been  a pretty sloppy contiuation, but they seem very durable.  I have been feeding mostly Reeds RG to them (2x a day...1ml worth at each feeding), with an occasional t-iso without issue.  We may miss a few days here and there but they always seem to bounce back very readily.
 
Been using 1 2.5 bucket with a flow through.  So I add 1 quart, 2x a day to the bucket of freshly prepared salt water.  This flows through to my Apocyclops panamensis to feed them. 
 
I have been cleaning the buckets about once every 4-6 weeks.  I have had two infestations of Apocyclops panamensis which was easily solved by seiving.