Culturing Journal DataSheet
This first post should be updated regularly to include new information as events take place or changes are made to your system
General Species: Oxyrrhis marina
Species description: heterotrophic dinoflagellate
Culture source (link if possible
): Andy Berry (Umm_fish?)
If algae, CCMP # (Optional
): http://ccmp.bigelow.edu/ Culture Establishment Date: 10/13/2011
Continuation Date: Culturing Vessel Details Salinity: 1.020
Temperature: ambient room temp (~78F)
pH: est. 7.7
Vessel description: 1g rubbermaid cereal boxes
Lighting description: ambient room lighting
Lighting cycle: natural day cycle, room lamp several feet away in the evenings
Aeration description: rigid air line to the bottom of the container, bubble count is fast, perhaps 10/sec
Methodologies Split methodology: harvest 1/5 of each container every couple of days, sieving through 54m mesh. What makes it through the mesh is fed to my A panamensis cultures.
Culture medium description: RGComplete is fed at 10 drops per gallon of culture, twice daily.
Cell count: Unable to estimate. Weekly samples are collected in a clear cup. I shine a flashlight through it and after a minute, the cloudy sample resolves into whorls and spiral galaxy looking clouds of individual specs.
Reference links: http://www.mbisite.org/Forums/tm.aspx?m=59814 Additional Information (No Pictures or Videos in the Section Please) Notes: You will be required to provide photographic evidence and as much detail as possible about your project in this thread.
If your thread does not contain detailed enough photos and information the MBI Council will not be able to approve your reports.