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Re: Breeding Journal, Species: [Paracanthurus hepatus]
Sunday, November 27, 2011 5:44 AM
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 Originally Posted by aomont I think they may be gone... There should be, at least, a visible larvae inside. If it was Z. flavescens they would be hatching actually.  I am incline to agree with you. I was hoping for a big cell division type of picture to be able to show that they are fertile and everything was good. Or a picture of a curled up fry (a bit like a clown still in its egg). On the other side, they continue to change, so as long as they keep doing stuff, Ill keep taking pics of them doing the stuff that they do.
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Re: Breeding Journal, Species: [Paracanthurus hepatus]
Sunday, November 27, 2011 7:24 AM
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That is a larvae developing in that egg !!!!!!!!!!!!!!!!!!!!! You're seen it right.  Also, look how clear it is compared to the others. I don't know what came first, the critters or the eggs opacity, I'm betting on the later one. but lots of infertile eggs may turn water conditions bad for the fertile ones. Wait moment and many more experienced folks will chime in here.
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Re: Breeding Journal, Species: [Paracanthurus hepatus]
Sunday, November 27, 2011 8:19 AM
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Yes,it is an embryo,definitely! Clear eggs are healthy,while hazy milky ones are dead.And you see lots of ciliates scavenging on them. Keep up with the good work,we all want to see P.hepatus prolarvae!
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Re: Breeding Journal, Species: [Paracanthurus hepatus]
Sunday, November 27, 2011 11:42 AM
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Re: Breeding Journal, Species: [Paracanthurus hepatus]
Sunday, November 27, 2011 11:59 AM
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Eggs and videos were taken approx 30 hours after spawn.
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Re: Breeding Journal, Species: [Paracanthurus hepatus]
Sunday, November 27, 2011 1:17 PM
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Fantastic work Darren! Good luck with your venture!
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Re: Breeding Journal, Species: [Paracanthurus hepatus]
Sunday, November 27, 2011 1:31 PM
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Awesome! Very cool indeed. 
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Re: Breeding Journal, Species: [Paracanthurus hepatus]
Sunday, November 27, 2011 1:35 PM
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awesome videos!!!! Very cool.
RLTW 180 Gallon Mixed Reef Then I heard the voice of the Lord saying, "Whom shall I send? And who will go for us?" And I said, "Here am I. Send me!" Isaiah 6:8
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Re: Breeding Journal, Species: [Paracanthurus hepatus]
Sunday, November 27, 2011 7:53 PM
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Originally Posted by luis a m Keep up with the good work,we all want to see P.hepatus prolarvae! ok, here you go Profile of Prolarvae  Top Down of Prolarvae  This morning all of the good/clear eggs appear to have hatched. I cannot find a single floating clear egg. I can however find lots of prolarvae. I counted 15 and then started taking some photos. They were near the surface of the water (less than 1cm deep), mostly stationary. When the container was bumped then a few would move. They would all react by swimming away if I put a pipette close to them. Total length for the two measured (separate prolarvae in each photo) was 2mm each. I do not have a hatch time so I dont know the period for spawn to hatch. These photos were taken 52 hours post spawn. All of the eggs that were assumed bad or questionable are unhatched in a separate container.
<message edited by CaptCrash on Monday, November 28, 2011 10:48 PM>
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Re: Breeding Journal, Species: [Paracanthurus hepatus]
Sunday, November 27, 2011 11:11 PM
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Wow, fantastic photos Capt! I'm excited just looking!
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Re: Breeding Journal, Species: [Paracanthurus hepatus]
Monday, November 28, 2011 5:01 AM
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I'm not expecting to be able to feed them, but it's a good start for me.
Does anyone have any suggestions on what I could feed them, it needs to be a lot smaller than a rotifer from what I have read.
I'm thinking some sort of coral foods?
Any suggestions are welcome
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Re: Breeding Journal, Species: [Paracanthurus hepatus]
Monday, November 28, 2011 4:20 PM
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Simply awesome!The feat and the quality of the pics  You say that you don´t use a microscope to take them? I am not aware of any previous report on captive beeding of this species.Somebody has seen any? See my threads on pigmy angels.As the challenge seems so unsurmountable,I divided the task in 3 stages. Obtaining many prolarvae Culturing them until I get many larvae. Raising the larvae. Seemingly in the 2nd stage,they like still water,and they are prone to bacterial kill outs. You could start by keeping them in a tray or in a dish. Wish you the best of lucks and we all are holding our breath! 
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Re: Breeding Journal, Species: [Paracanthurus hepatus]
Monday, November 28, 2011 6:34 PM
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Originally Posted by luis a m
You say that you don´t use a microscope to take them?
Nope, its a digital SLR and an older one at that. Here is a couple of pictures of how I am storing the prolarvae (its a container of water with a light).  And of the camera, macro lens and extension tubes. 
Originally Posted by luis a m I am not aware of any previous report on captive beeding of this species.Somebody has seen any? See my threads on pigmy angels.As the challenge seems so unsurmountable,I divided the task in 3 stages. Obtaining many prolarvae Culturing them until I get many larvae. Raising the larvae. Seemingly in the 2nd stage,they like still water,and they are prone to bacterial kill outs. You could start by keeping them in a tray or in a dish. Wish you the best of lucks and we all are holding our breath! at 72 hours post hatch spawn  at 81 hours post hatch spawn  EDIT: updated the text from hatch to spawn. I wrote the wrong one.
<message edited by CaptCrash on Monday, November 28, 2011 10:37 PM>
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Re: Breeding Journal, Species: [Paracanthurus hepatus]
Monday, November 28, 2011 8:09 PM
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Hey Captain are you using post hatch or post spawn time in the pictures ? In the first two you used post-spawn and the last ones, post hatch. Also it hasn't passed 3 days since hatch. Anyway, the development pictures are exciting !
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Re: Breeding Journal, Species: [Paracanthurus hepatus]
Monday, November 28, 2011 9:15 PM
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This is simply awesome! Keep up the great work. I know it may not help, and I haven't skimmed all the comments, but if you didn't already see it, there was a rumored report of success with this species using ciliates as a first food. While we lack the "proof" we all want (namely photographs), I wouldn't fully discount the report either - http://reefbuilders.com/2011/08/19/hippo-hepatus-blue-tang-taiwan/ I think what's most amazing is that they're spawning in such a small tank!
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Re: Breeding Journal, Species: [Paracanthurus hepatus]
Monday, November 28, 2011 10:38 PM
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Originally Posted by aomont Hey Captain are you using post hatch or post spawn time in the pictures ? In the first two you used post-spawn and the last ones, post hatch. Also it hasn't passed 3 days since hatch. Anyway, the development pictures are exciting ! whoops, I listed the wrong one but you figured it out. Its from spawn.
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Re: Breeding Journal, Species: [Paracanthurus hepatus]
Tuesday, November 29, 2011 9:28 AM
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The following photos were the hardest to take so far. The prolarvae are very active now when I try to take photos. Previously they were reasonable still and quite calm. Today, not a chance. The following photos are the best from about 40 attempts. Most are a blurred mess or have overlapping images of the same prolarvae two or three times. Photos at 4 days from spawn   Side lighting to show internals  I am going to separate the prolarvae tomorrow into 4-6 separate containers. I will then add "food" of various sorts to each container plus a control with no added food. We can then see if any of the food is good (or just makes the water bad really quick). My intentions are to add the following, one to each container. 1. Tetraselmis 2. Nannochlorpus 3. Rotifers (I know they are too big but its an experiment, maybe larvae will work?) 4. Coral food, the smallest I can find (yet to be decided) 5. Golden Pearls 5-50um fry food 6. Control (No Addition) The downsides are that the algae that I have is Reeds frozen stuff so its not mobile, but its better than nothing. If anyone has any suggestions, please pipe up now.
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Re: Breeding Journal, Species: [Paracanthurus hepatus]
Tuesday, November 29, 2011 10:54 AM
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Pics are good enough to see your progress And you are not noticing bacterial attack.Which is seen as milky dead PL which are "reabsorved",like burned in acid. The mouth and cloaca are almost finished and otoliths can be seen.I am not sure what the opaque tube that runs behind the yolksac is? I wouldn´t start offering food before the eyes are pigmented.
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Re: Breeding Journal, Species: [Paracanthurus hepatus]
Tuesday, November 29, 2011 11:14 AM
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Originally Posted by luis a m I wouldn´t start offering food before the eyes are pigmented. Cool thanks for that. Ill wait till I can see the pigment.
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Re: Breeding Journal, Species: [Paracanthurus hepatus]
Tuesday, November 29, 2011 12:53 PM
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possibly add some salina moina to that list?
granted that the adults would be farly too large to eat but the newly born one may do the trick
just an idea
i know that when i was raising celestial peral danios i had to culture paramecium for the fry
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